Control gene of paddy leaf shape and application thereof

A gene, rice technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc.

Inactive Publication Date: 2007-03-07
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The programmed death of sclerenchyma elements in rice leaves is still a blank in the research, but previous studies have shown that programmed cell deat

Method used

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  • Control gene of paddy leaf shape and application thereof
  • Control gene of paddy leaf shape and application thereof
  • Control gene of paddy leaf shape and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1. Rice material

[0049] Rice (Oryza sativa L.) mutant Shallot-like leaf1 (sll1), the original wild-type material is "Nipponbare" japonica rice variety.

[0050] 2. Analyze and locate groups

[0051] The homozygous sll1 mutant was crossed with the original wild-type variety Nanjing 6, and the F1 generation was selfed to obtain the F2 population, and 711 sll1 mutant individuals were selected as the mapping population. At the beginning of tillering, take about 1 gram of young leaves from each plant to extract total DNA.

[0052] 3. SSR and STS markers to locate the SLL1 gene

[0053] The rapid extraction method of rice trace DNA is used to extract genomic DNA for gene mapping from rice leaves. Take about 100 mg of rice leaves, freeze them with liquid nitrogen, grind them into a powder in a small mortar with a diameter of 5 cm, and transfer them to a 1.5 ml centrifuge tube to extract DNA. The obtained DNA precipitate is dissolved in 100 μl of ultrapure water. Use 2μl DNA samp...

Embodiment 2

[0061] Plant transformation:

[0062] The BAC clone B1040D06 was completely digested with BglII and SSe8387I. After electrophoresis, a DNA fragment of about 8KB was cut and connected to the BamHI and PstI cleavage points in the multiple cloning site of pCAMBIA1300. This DNA fragment covers the entire ORF. The genomic region also includes the 1.6KB promoter sequence upstream of ATG.

[0063] This plasmid was transformed into Agrobacterium (Agrobacterium tumefaciens) strain EHA105 to transform rice by electroporation. We use callus induced by mutant immature embryos, after 3 weeks of inducing medium culture, select vigorously growing callus as the recipient of transformation. The rice callus was infected with the EHA105 strain containing the binary plasmid vector, and after co-cultivation in the dark at 25°C for 3 days, it was cultured on a selection medium containing 40 mg / L Hygromycin. The selection of resistant callus was cultured on predifferentiation medium containing 50mg / L fo...

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PUM

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Abstract

the invention discloses a rice leaf shape controlled protein coded by SLL1 gene with amino acid sequence as follow as Seq ID No:2 as well as nucleic acid sequence in the Seq ID No:1 and plasmid, plant express carrier and host cell with the related gene. The invention also provides culturing method of coiled plant leaf, which transforms plant carrier into plant cell to form plant. The new gene SLL1 is cloned from coiled rice leaf mutant to code transcription factor of MYB-DNA connecting area of SHAQKYF, which controls coiling degree through moduling the programming death in the vascular tissue differentiation course.

Description

Technical field [0001] The invention belongs to the field of plant genetic engineering. Specifically, the present invention relates to a method of cloning the rice SLL1 (Shallot Like Leaf 1) gene using map-based cloning technology, and using transgene complementation experiments to identify the function of the gene; at the same time, it also relates to using the gene to regulate leaf thick-walled fibrous tissue, thereby By adjusting the internal structure of rice leaves, the number of mesophyll cells and chlorophyll content of crops are increased; at the same time, the gene is used to adjust leaf curling to obtain ideal plant types of crops and increase crop yields. Background technique [0002] As a model plant of monocots, rice has the typical characteristics of monocot leaves. Rice leaves are composed of leaves and sheaths. The leaves can be divided into three components: epidermis layer, mesophyll layer and vascular layer. The vascular layer includes the phloem near the abaxi...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N15/82C12N1/21C12N5/10
Inventor 钱前薛红卫张光恒胥倩朱旭东徐淑平曾大力郭龙彪胡江
Owner CHINA NAT RICE RES INST
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