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Nanometre collagen oligopeptide and its producing method

A nano-glue and oligopeptide technology, which is applied in the field of nano-collagen oligopeptides and its preparation, can solve the problems of low skin permeability and no nutritional value of collagen oligopeptides, so as to improve immunity, improve bioavailability, The effect of enhancing collagen activity

Active Publication Date: 2007-05-23
杜以波 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] In the above technology, although the molecular weight of the hydrolyzed collagen product is relatively small, the skin permeability is still not high. Because the molecular weight is still relatively large, it mainly plays the role of care for the outer layer in cosmetics, and it is difficult to penetrate into the dermis and really play the role of skin care. care, repair
[0014] The molecular weight of the above products cannot be regarded as collagen oligopeptides (dipeptides to heptapeptides) in the strict sense, so they do not have the nutritional value of collagen oligopeptides

Method used

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  • Nanometre collagen oligopeptide and its producing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: After the fresh pigskin is depilated, it is scraped, washed, and crushed into small pieces, soaked in 10 times the volume of saturated lime water for 40 days, rinsed with water, washed until nearly neutral, and then washed with 10 times the volume 1M HCl, soaked for 48 hours, washed with water until the pH is equal to about 4, to obtain pretreated pigskin. Take 200g of pretreated pigskin, put it into the reaction bottle of the rotary evaporator, add 1800g of water, add 2g of pepsin and 2g of trypsin respectively, keep a vacuum of 1-10KPa, hydrolyze at a constant temperature of 50°C for 4 hours, and then add 2g of papain , adjusted the pH value to 5 with 0.1M NaOH, and hydrolyzed at 60°C for 3 hours under the same vacuum condition. Then add 2 grams of matrix metalloproteinase, continue to keep constant temperature at 60° C., and hydrolyze under the same vacuum condition for 3 hours. Heat to 100°C for 10 minutes, cool to 60°C, filter with absorbent cotton, and...

Embodiment 2

[0049] Fig. 1 is a schematic diagram of the molecular weight distribution of the nano-collagen oligopeptide detected by electrospray mass spectrometry (ESI / MS). The results of ESI / MS analysis show that 90% of the molecular weight of the product is distributed between 100 and 1000D, which is a combination of small molecules from dipeptide to heptapeptide. The product is a collagen oligopeptide, and its molecular size and length should be between a few nanometers and a dozen nanometers, which is a nanoscale molecule, so the hydrolyzed product is a nano-collagen oligopeptide. Example 2: After the fresh pigskin is depilated, it is scraped, washed, and crushed into small pieces, soaked in 10 times the volume of saturated lime water for 40 days, rinsed with water, washed until nearly neutral, and then washed with 10 times the volume 1M HCl, soaked for 48 hours, washed with water until the pH is equal to about 4, to obtain pretreated pigskin. Take 200g of pretreated pigskin, put it ...

Embodiment 3

[0051] Example 3: After the fresh pigskin is depilated, it is scraped off, washed, and crushed into small pieces, soaked in 10 times the volume of saturated lime water for 40 days, rinsed with water, washed until nearly neutral, and then washed with 10 times the volume 1M HCl, soaked for 48 hours, and the water sank to a pH equal to about 4 to obtain pretreated pigskin. Take 200g of pretreated pigskin, put it into the reaction bottle of the rotary evaporator, add 1800g of water, add 2g of pepsin and 2g of trypsin respectively, keep a vacuum of 10-30KPa, hydrolyze at a constant temperature of 40°C for 4 hours, then add 2g of papain , use 0.1M NaOH to wither the pH value to 5, and under the same vacuum condition, hydrolyze at a constant temperature of 70°C for 3 hours. Then add 2 grams of matrix metalloproteinase, continue to keep constant temperature at 60° C., and hydrolyze under the same vacuum condition for 3 hours. Heat to 100°C for 10 minutes, cool to 60°C, filter with ab...

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Abstract

This invention relates to a one-step method for extracting nano-collagen oligopeptides from skin of animal directly. The said method includes enzymolyzing collagen protein-containing natural animal skin directly to obtain small molecule collagens with average molecular weight of about 500D and distributing mainly between 100-1000D, combination of dipeptides to heptapeptides. Molecules of Gaussiandistribution are tripeptides, tetrapeptides and pentapeptides. Amino acid compositions of collagen are glycine 24%, praline 14%, hydroxyproline 11%, glutamic acid 9%, and arginine 8%. The extracted nano-collagen oligopeptides can be served as the raw materials of good medicine, health care products, and cosmetics.

Description

technical field [0001] The invention relates to a preparation method for directly extracting nano-collagen oligopeptides from animal skins. Specifically, the natural animal skins containing collagen are directly enzymolyzed to obtain small-molecule nano-collagen oligopeptides. The nano-collagen oligopeptide extracted by the method can be used as a good raw material for medicine, health care products and cosmetics. Background technique [0002] Collagen (Collagen) described in the present invention claims collagen again, and it is the important protein of animal connective tissue, and connective tissue except containing 60~70% of moisture, collagen accounts for about 20~30%, because there is high content Collagen and connective tissue have certain structural and mechanical properties, such as tensile strength, tension, elasticity, etc., to achieve the functions of support and protection. About 16% of the human body is protein, and collagen accounts for 30-40% of the total pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/12C07K5/06C07K5/08C07K5/10C07K7/06A61K38/05A61K38/06A61K38/07A61K38/08A61K35/36A61P19/02A61P17/00A61P1/04
Inventor 杜以波钟望红
Owner 杜以波
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