Prostate cancer markers

a prostate cancer and marker technology, applied in the field of prostate cancer markers, can solve the problem that the change in psa levels is not useful in detecting individual cases of prostate cancer, and achieve the effect of low cost and availability

Inactive Publication Date: 2004-12-16
INCYTE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

0107] Animal models may be used as bioassays where they exhibit a phenotypic response similar to that of humans and where exposure conditions are relevant to human exposures. Mammals are the most common models, and most infectious agent, cancer, drug, and toxicity studies are performed on rodents such as rats or nice because of low cost, availability, lifespan, reproductive potential, and abundant reference...

Problems solved by technology

However, in its most advanced state, cancer growth becomes androgen-independent and there is currently no known treatment for this condition.
However, since PSA levels are also influe...

Method used

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examples

[0117] I Construction of cDNA Libraries

[0118] RNA was purchased from Clontech Laboratories (Palo Alto Calif.) or isolated from various tissues. Some tissues were homogenized and lysed in guanidinium isothiocyanate, while others were homogenized and lysed in phenol or in a suitable mixture of denaturants, such as TRIZOL reagent (Life Technologies, Rockville Md.). The resulting lysates were centrifuged over CsCl cushions or extracted with chloroform. RNA was precipitated with either isopropanol or ethanol and sodium acetate, or by other routine methods.

[0119] Phenol extraction and precipitation of RNA were repeated as necessary to increase RNA purity. In most cases, RNA was treated with DNase. For most libraries, poly(A) RNA was isolated using oligo d(T)-coupled paramagnetic particles (Promega), OLIGOTEX latex particles (Qiagen, Valencia Calif.), or an OLIGOTEX mRNA purification kit (Qiagen). Alternatively, poly(A) RNA was isolated directly from tissue lysates using other kits, includ...

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PUM

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Abstract

The present invention relates to a composition comprising a plurality of cDNAs which are differentially expressed in prostate cancer and which may be used in their entirety or in part as to diagnose, to stage to treat or to monitor the treatment of a subject with prostate cancer.

Description

[0001] This application is a continuation of U.S. application Ser. No. 09 / 919,172, filed Jul. 30, 2001, which further claims the benefit of U.S. Provisional Application Ser. No. 60 / 222,469, filed Jul. 28, 2000.[0002] The present invention relates to a composition comprising a plurality of cDNAs which are differentially expressed in prostate cancer and which may be used entirely or in part to diagnose, to stage, to treat, or to monitor the progression or treatment of prostate cancer.[0003] Array technology can provide a simple way to explore the expression of a single polymorphic gene or the expression profile of a large number of related or unrelated genes. When the expression of a single gene is examined, arrays are employed to detect the expression of a specific gene or its variants. When an expression profile is examined, arrays provide a platform for examining which genes are tissue specific, carrying out housekeeping functions, parts of a signaling cascade, or specifically rela...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/574
CPCC12Q1/6837C12Q1/6886C12Q2600/158G01N33/57434C12Q2600/136
Inventor FARIS, MARYTURNER, CHRISTOPHER M.
Owner INCYTE
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