A portable seawater nutrient salt automatic analysis device and its use method, wherein, the device includes a main casing and an automatic sampling and colorimetric analysis module inside the main casing, and a reagent setting is arranged on the outer wall of the main casing. The storage tank, waste liquid bag hook, control panel, power supply plug, communication connector and power supply control switch, the control panel, reagent storage tank, power supply plug and communication connector are connected to the automatic sampling and colorimetric analysis module, and the power supply control switch is connected to the power supply Plug connection, automatic sampling and colorimetric analysis module is provided with a second sampling pipeline and waste liquid discharge pipeline. Based on the concept of modular design, the present invention has a simple and compact structure, and is flexible and convenient to use. It can not only avoid property changes and measurement errors of samples due to storage, transportation, and manual access, but also greatly improve the work efficiency of detection and analysis. It is suitable for online Fully automatic measurement of original nutrient samples under different needs and conditions on board, fixed platform, and laboratory.