Biological material solid carrier technology and nano bioreactor synthesized therefrom
A technology of biomaterials and surfactants, applied in the direction of immobilization on or in the inorganic carrier, immobilization on/in the organic carrier, etc., can solve the problem of affecting the diffusion of reactants, limiting wide applicability, unstable pore structure and other problems, to achieve the effect of eliminating environmental pollution, not easy to adapt to biology, and improving catalytic activity
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Embodiment 1
[0017] Example 1: Preparation of a nanoreactor with high catalytic activity and high stability embedded with trypsin
[0018] Use butyl orthosilicate as the silicon source, and use P123 as the surfactant. Ingredient SiO for Synthesis of Mesoporous Silicon Structure 2 / surfactant / CH 3 CH 2 OH / H 2 The mass ratio of O / trypsin was 1 / 1.46 / 15 / 1.5 / 0.2.
[0019] First, a surfactant is dissolved in ethanol, and then a silicon source is added to it. After the solution was stirred and clarified, HCl (hydrochloric acid) was added dropwise to make it fully hydrolyzed, so that the pH value was 6-8. The obtained sol was vigorously stirred at 20-25°C for 4-10 hours, put into a vacuum device and stirred while vacuuming for 12-72 hours until the solution became a transparent and viscous sol; then mixed at 0-30°C The protease is stirred gently and continues to react under vacuum condition for 4-100 hours. Finally, the sample was washed several times with an alcohol-water mixture (1 / 50) to...
Embodiment 2
[0022] Example 2: Preparation of Nanoreactor with High Catalytic Activity and High Stability Embedding Fumarase
[0023] By the ratio of the corresponding raw material of embodiment 1, tensio-active agent, water, the scope is identical with embodiment 1. Process is also substantially the same as in Example 1. The immobilized enzyme is fumarase, SiO 2 / fumarinase mass ratio is 1 / 0.05.
[0024] The same nanoreactor has high activity and stability. As shown in Table 1, the initial activity was close to that of the pure enzyme, and its activity was still close to the initial activity after repeated use for 30 times.
Embodiment 3
[0025] Example 3: Preparation of nanoreactor with high catalytic activity and high stability of embedded lipase
[0026] By the ratio of the corresponding raw material of embodiment 1, tensio-active agent, water, the scope is identical with embodiment 1. Process is substantially the same as in Example 1. The immobilized enzyme is lipase, SiO 2 / lipase mass ratio is 1 / 0.4.
[0027] The same nanoreactor has high activity and stability. As shown in Table 1, the initial activity was close to that of the pure enzyme, and its activity was still close to the initial activity after repeated use for 30 times.
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