Method for adsorbing stalk cellulose of enzymolysisby using cellulase

A technology of straw cellulose and cellulase, applied in the field of cellulose enzymatic hydrolysis, can solve the problems of low utilization rate of cellulase, achieve the effect of shortening the enzymatic hydrolysis time and saving energy

Inactive Publication Date: 2007-07-25
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of low cellulase utilization rate in the current cellulose enzymatic hydrolysis process, and propose a cellulos

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Put 15g of wheat straw with a water content of 10% into a steam explosion tank and treat it for 5 minutes at a steam pressure of 1.0Mpa, then release it under reduced pressure instantaneously to obtain the wheat straw after steam explosion treatment, and dry it at 60°C 48 hours;

[0028] 2. Mix the dried steam-exploded wheat straw with the cellulase liquid (the cellulase liquid prepared by the acetate buffer solution with a pH of 4.8) according to the solid-liquid weight ratio of 1:7, and the cellulase dosage is 30IU / g steam-exploded wheat straw, after adsorption at -1°C for 2 hours, the system was separated;

[0029] 3. Restore the solid-liquid ratio (solid-liquid weight ratio: 1:7) of the substrate adsorbed by the enzyme, and perform enzymatic hydrolysis at 50° C. for 72 hours.

[0030] The enzyme used in this example is Trichoderma reesei, and the amount of reducing sugar produced per unit enzyme is 22.62 mg.

Embodiment 2

[0032] 1. Put 15g of sorghum stalks with a water content of 10% into a steam explosion tank and treat them for 6 minutes under a steam pressure of 1.4Mpa, then release them under pressure instantly to obtain steam-exploded sorghum stalks, and heat them at 60°C dry for 48 hours;

[0033] 2. Mix the dried steam-exploded sorghum stalks with cellulase (enzyme liquid prepared from a citric acid buffer solution with a pH of 4.8) according to the solid-liquid weight ratio of 1:6, and the cellulase dosage is 50IU / g Substrate, system separation after adsorption at -3°C for 2 hours;

[0034] 3. Add fresh steam-exploded sorghum stalks to the supernatant separated in step 2, so that the weight ratio of the mixed liquid to solid and liquid is 1:6, and then perform adsorption-desorption at -3°C;

[0035] After the adsorption-desorption reaches equilibrium, the solid-liquid separation is carried out to obtain the supernatant and the steam-exploded straw adsorbed with cellulase; the steam-ex...

Embodiment 3

[0038] 1. Put 15g of rice straw with a water content of 35% into a steam explosion tank and treat it under a steam pressure of 1.1Mpa for 7 minutes, then release it under instantaneous pressure reduction to obtain the rice straw after steam explosion treatment, and dry it at 60°C 48 hours;

[0039] 2. Mix the dried steam-exploded rice straw with cellulase (enzyme liquid prepared by a sodium acetate buffer solution with a pH of 4.8) according to the weight ratio of solid to liquid in a ratio of 1:8, and the amount of cellulase is 100IU / g Substrate, system separation after adsorption at 0°C for 3 hours;

[0040] 3. This embodiment also includes repeating the following steps 2 times: adding fresh steam-exploded sorghum stalks to the supernatant obtained in step 2, so that the weight ratio of the mixed liquid to solid liquid is 1:8. After 4 hours of adsorption-desorption, solid-liquid separation was carried out to obtain the supernatant and the steam-exploded straw adsorbed with ...

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PUM

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Abstract

This invention relates to a method for preparing straw cellulose by adsorption-enzymolysis with cellulose. The method comprises: (1) steam-exploding straw, drying, mixing with cellulose solution, performing adsorption-desorption at -4-0 deg.C, and performing solid-liquid separation to obtain supernatant and cellulose-adsorbed steam-exploded straw; (2) performing enzymolysis on the straw at 40-50 deg.C for 48-72 h; (3) repeating adsorption-desorption and enzymolysis for 1-4 times. The cellulase consumption is 6 IU/g substrate, and the productivity of reducing sugar is 17.70-27.07 mg/IU cellulase. The method also has such advantages as short enzymolysis time, low energy consumption and recoverable cellulose.

Description

technical field [0001] The invention belongs to the field of cellulose enzymatic hydrolysis, in particular to a method for using cellulase to absorb and enzymatically decompose straw cellulose, so as to make efficient use of cellulase. Background technique [0002] my country is rich in available plant cellulose resources, and the annual output of crop straw alone can reach as much as 700 million tons. The research on cellulose saccharification started nearly two hundred years ago. Early studies mainly used concentrated acid method and dilute acid method to hydrolyze saccharified cellulose into glucose. The acid hydrolysis method has the biggest advantage of fast reaction speed, but due to the consumption of a large amount of acid, the corrosion of the reaction equipment, and the high energy consumption, the sugar produced by acid hydrolysis is easy to degrade and usually produces uronic acid or furfural, etc. Unfavorable by-products of fermentation or processing. Althoug...

Claims

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Application Information

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IPC IPC(8): C12P19/02C12R1/685C12R1/645
CPCY02P20/59
Inventor 陈洪章徐建
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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