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Micro-fluidic chip containing sample pre-treatment film and production method therefor

A sample pretreatment, microfluidic chip technology, applied in the preparation of test samples, instruments, measuring devices, etc., can solve the problems of complex micromachining and operation methods, low pretreatment efficiency, high cost, and achieve detection selectivity and improved analytical sensitivity, simple preparation and low cost

Inactive Publication Date: 2007-08-01
CHONGQING UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] Aiming at the pretreatment methods of biological macromolecules in the microfluidic chip system at the present stage, the commonly used methods are: using micro-electromechanical technology to process micro-devices such as weirs, fences, columns, and sieves in the micro-channel of the chip to form a filter pre-processing method. Processing chips, but the micro-processing and operation methods are complicated and costly; by etching microchannels in the chip and introducing different forms of sorbents such as open tubes, packed columns and monolithic columns to form solid-phase extraction pretreatment chips, this method The selectivity of the analysis can be greatly improved by selecting adsorbents with different properties, but the pretreatment efficiency is also reduced due to the elution process; the local application of an electric field, magnetic field or sound field to the chip constitutes a pretreatment based on the principle of the electric field, magnetic field or sound field. Handle the chip, but it is also easy to cause damage to the structure of the small molecule to be tested

Method used

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  • Micro-fluidic chip containing sample pre-treatment film and production method therefor

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Embodiment 1

[0018] Example 1: Microfluidic chip containing sample pretreatment membrane

[0019] Modification method of polyamide membrane 3: take 10 polyamide membranes with a diameter of 5mm, and put them in 2.5mol L at 30°C -1 Activated in HCl solution, the activated membrane is washed with distilled water: then, add the dye Cymbal blue F-3GA and the activated polyamide membrane in a closed container, and react at 60°C for 30 minutes, then add NaCl to accelerate the dye deposition on the film. After continuing to react for 30min, add Na 2 CO 3 , heated up to 80°C, and reacted for 4 hours. During the reaction process, the reaction solution was continuously oscillated to make the ligand bonding on the surface of the membrane uniform. After the reaction, the dye-coupled polyamide membrane was cleaned with 80% ethanol and distilled water until there was no dye in the cleaning solution, and then the membrane was removed. Store in acetic acid-sodium acetate buffer solution containing 0.0...

Embodiment 2

[0023] Example 2: Detection application of microfluidic chip containing sample pretreatment membrane

[0024] Will contain 0.10μg·mL -1 The serum sample of dobutamine hydrochloride is injected from the injection pump through the capillary, and the chemiluminescent reagent KMnO 4 With Luminol (both concentrations are 2.0×10 -6 mol L -1 ,) reagents are introduced into the chip from the liquid guide holes 5 and 6 respectively, and after the two are mixed through the cross-shaped intersection point, a chemiluminescence reaction occurs, and a chemiluminescence signal is obtained from the detection window D. Through the comparison of the luminescence results, it can be seen that after passing through the pretreated affinity membrane, the chemiluminescence signal response sensitivity of dobutamine hydrochloride is about 2.10 times higher than that of the injection without the membrane, compared with five times on the microfluidic chip. The measured relative standard deviation RSD,...

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Abstract

This invention relates to one micro flow control chip and its process method with sample pre-process film, wherein, the chip is composed of bottom slice and cover slice; the bottom slice uses glass, silicon, PMMA or PDMS as basic materials to etch flow mixture and reaction micro channel network; the cover slice adopts PDMS process sealed to bottom slice; in the relative position of each channel opening liquid guide hole for micro flow; putting sample process film in the pointing position to sample entrance imbedded into cover slice.

Description

technical field [0001] The invention belongs to the technical field of miniature total analytical system (μ-TAS, Miniaturized Total Analytical System), in particular to a microfluidic chip that can be used for purification or enrichment of biological fluid samples. Background technique [0002] Micro-total analysis system (μ-TAS, also known as lab-on-a-chip), can greatly reduce the consumption of reagents and shorten the Analysis time, improve analysis and detection efficiency. After more than ten years of development, μ-TAS has been widely used in disease diagnosis, biochemical analysis, clinical detection and other fields. At present, the biochemical detection system with blood, urine and other body fluids as the main body of the sample is one of the main application objects of μ-TAS. Its substrate and components are complex, and the matrix effect coexists with various interfering components. Most of the sample pretreatment process is realized outside the microchip throu...

Claims

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Application Information

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IPC IPC(8): G01N1/34G01N30/08
Inventor 徐溢张剑郑勇季金苟温志渝
Owner CHONGQING UNIV
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