Streptomycete S1-5 and uses thereof

A technology of streptomyces and plant pathogenic fungi, applied in the direction of application, bacteria, biocides, etc., can solve the problems of no streptomyces and achieve the effects of less resistance to drugs, lower agricultural costs, and high killing activity

Inactive Publication Date: 2008-05-21
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, there is no report that Streptomyces sp. can simultaneously control plant pathogenic fungi and kill acarids

Method used

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  • Streptomycete S1-5 and uses thereof
  • Streptomycete S1-5 and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Obtaining fermentation broth

[0027] (1) Slope culture: Use PDA medium, coat the slant with Streptomyces S1-5 strain, and cultivate for 2 to 3 days at 30°C to obtain the slant strain;

[0028] (2) Seed culture: inoculate the slant strains to Gao's No. 1 medium and cultivate for 2 to 3 days at 30°C to obtain Streptomyces S1-5 colonies;

[0029] (3) Fermentation culture: Preparation of original fermentation medium: 20 mL of 1% soybean meal powder infusion, 20 g glucose, 3.0 g peptone, 2.0 g calcium carbonate, 2.5 g sodium chloride, and 1000 ml distilled water. Scrape the spores from the colony of Streptomyces S1-5 cultured on Gao's medium No. 1 and place them in sterile water, shake for 10 minutes, and make a concentration of 1×10 8 The spore suspension per ml was inserted into the fermentation medium at a 10% (V / V) inoculum amount, and a 250 ml Erlenmeyer flask was placed on a shaker and cultured on a constant temperature shaker for 5 days to obtain a fermentatio...

Embodiment 2

[0030] Example 2: Obtainment and determination of fermentation broth extract

[0031] Take 50ml of the fermentation broth, centrifuge at 5000r / min for 10min, and add an equal volume of absolute ethanol to the supernatant to form the crude extract of the biologically active substance supernatant. The filter cake was washed twice with distilled water and centrifuged, the supernatant was discarded, the filter cake was reduced to the volume of the fermentation broth by adding absolute ethanol, after soaking for 1 hour, centrifuged at 5000r / min for 10 minutes, and the supernatant was taken as the crude mycelium of the biologically active substance. Extract. The two crude extracts and the supernatant after centrifugation of the fermentation broth were tested for bacteriostasis and acaricidal tests, and the same ethanol aqueous solution was used as a control.

[0032] Antibacterial effect determination method: (Four kinds of plant pathogenic fungi are gifted by the Department of Plant Pr...

Embodiment 3

[0041] Example 3: Obtainment and determination of biologically active substances

[0042] After the fermentation broth of Streptomyces S1-5 is centrifuged, the supernatant is taken, and an equal volume of ethyl acetate is added for extraction, and the upper layer is concentrated under reduced pressure and collected. Collect the lower layer for bacteriostasis determination, use ethyl acetate as the eluent to run the column, the eluent is petroleum ether / ethyl acetate, column chromatography silica gel (200-300 mesh) as the stationary phase, use the dry method to pack the column, Collect the eluent at the lower end of the test tube, 10 mL per tube, 10 tubes in total. Biological activity tracking: the collected 10 tubes of eluate and the extracted lower layer solution were used filter paper method to determine their bacteriostasis against the test bacteria (Alternaria longifolia and Rhizoctonia solani, courtesy of the Department of Plant Protection, Zhejiang University) Effect (result...

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Abstract

The invention provides a new bacterial strain that can prevent and control plant pathogenic fungi and kill acarids—streptomyces S1-5 and its application in resisting plant pathogenic fungi and killing acarids. Streptomyces sp.S1-5 (Streptomyces sp.S1-5), preserved in China Center for Type Culture Collection, date of preservation: May 24, 2007, preservation number CCTCC No: M 207073. The pesticide prepared by the present invention can achieve the effect of killing fungicides and killing acarids by using it once, greatly reducing agricultural costs and lightening the burden on farmers; and the duration of effect is as long as 30 days, and it is also safe for humans and animals, does not kill natural enemies and is beneficial Insects are not easy to produce resistance to control objects, do not pollute the environment, can be rapidly degraded by soil microorganisms without affecting soil fertility and microbial flora, have no bioaccumulation, and are very safe for ecology. They are a key enzyme inhibitor. Pesticides, no cross-resistance with other insecticides.

Description

(1) Technical field [0001] The present invention relates to a new strain capable of controlling phytopathogenic fungi and killing mites—Streptomyces sp.S1-5 and its application in resisting phytopathogenic fungi and killing mites, and using Streptomyces S1 -5 The prepared emulsion can be used to resist plant pathogenic fungi and kill mites. (2) Background technology [0002] Biological pesticides are recognized as green pesticides and ideal pesticides in the 21st century for their low toxicity, no residue, no pollution, and easy availability of raw materials. The development of new biological pesticides is undoubtedly an advanced behavior with both social and economic benefits. Mites and phytopathogenic fungi are the main hazards to various economic crops such as vegetables, tea, fruit trees and cotton. According to statistics, the country only needs to prevent and control mites in an area of ​​no less than 100 million acres, causing annual yield losses of 12%-20%. More than 25% ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P7/04C12R1/465
Inventor 吴石金
Owner ZHEJIANG UNIV OF TECH
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