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Streptomycete S1-5 and uses thereof

A technology for streptomyces and phytopathogenic fungi, applied in the application, bacteria, biocide and other directions, can solve the problem of no streptomyces, and achieve the effects of not easy drug resistance, fast efficacy, and high killing activity

Inactive Publication Date: 2011-05-25
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, there is no report that Streptomyces sp. can simultaneously control plant pathogenic fungi and kill acarids

Method used

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  • Streptomycete S1-5 and uses thereof
  • Streptomycete S1-5 and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: the acquisition of fermented liquid

[0027] (1) Incline cultivation: adopt PDA medium, apply Streptomyces S1-5 strain on the slope, and cultivate at 30° C. for 2 to 3 days to obtain the strain on the slope;

[0028] (2) Seed culture: inoculate the slant bacteria into Gaoshi No. 1 medium, cultivate at 30° C. for 2 to 3 days, and obtain Streptomyces S1-5 colonies;

[0029] (3) Fermentation culture: original fermentation medium preparation: 20 mL of 1% soybean cake powder infusion, 20 g of glucose, 3.0 g of peptone, 2.0 g of calcium carbonate, 2.5 g of sodium chloride, and 1000 ml of distilled water. Scrape the spores from the colony of Streptomyces S1-5 cultured on Gao’s No. 1 medium, place them in sterile water, shake for 10 minutes, and make a concentration of 1×10 8 The spore suspension of each / ml was inserted in the fermentation medium with 10% (V / V) inoculum, placed on a 250ml Erlenmeyer flask on a shaker, and placed in a constant temperature shaker t...

Embodiment 2

[0030] Embodiment 2: Obtaining and measuring of fermented liquid extract

[0031] Take 50ml of fermentation broth, centrifuge at 5000r / min for 10min, add an equal volume of absolute ethanol to the supernatant to obtain the crude extract of the supernatant of biologically active substances. Wash the filter cake twice with distilled water and centrifuge, discard the supernatant, add absolute ethanol to the filter cake to reduce to the volume of the fermentation broth, soak for 1 hour, centrifuge at 5000r / min for 10 minutes, and take the supernatant, which is the crude mycelium of the biologically active substance. Extract. The two kinds of crude extracts and the centrifuged supernatant of the fermented liquid were used for the determination of antibacterial effect and acaricidal test, and the same ethanol aqueous solution was used as a control at the same time.

[0032] Determination method of antibacterial effect: (four kinds of plant pathogenic fungi are donated by the Plant ...

Embodiment 3

[0041] Embodiment 3: Obtaining and measuring of biologically active substances

[0042] After the Streptomyces S1-5 fermentation broth was centrifuged, the supernatant was taken, added an equal volume of ethyl acetate, extracted, and the upper layer was concentrated and collected under reduced pressure. Collect the lower layer for antibacterial determination, use ethyl acetate as the eluent to walk the column, the eluent is petroleum ether / ethyl acetate, column chromatography silica gel (200-300 mesh) is used as the stationary phase, and the column is packed in a dry method. Use the test tube to collect the eluate at the lower end, collect 10 mL in each tube, and collect 10 tubes in total. Tracking of biological activity: The collected 10 tubes of eluate and the extracted lower layer solution were respectively tested for their antibacterial activity against the tested bacteria (Calleatherium longopodium and Rhizoctonia solani, donated by the Plant Protection Department of Zhej...

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Abstract

The invention provides a novel strain of Streptomyces sp. S1-5 which is capable of controlling and preventing plant pathogenic fungi and killing mite and the application in the anti-plant pathogenic fungi and mite killing. The Streptomyces sp. S1-5 is stored in China Center for Type Culture Collection on 24 May, 2007. The preservation number of the strain is CCTCC No: M207073. Only one time application of the pesticide made by the invention can reach the effect of anti-plant pathogenic fungi and anti-mite; the invention decreases agriculture cost and reduces loads of peasant; the duration period against mites is 30 days; the invention also has the advantages that the strain is safe to human beings and animals which does not kill the natural enemy and the beneficial insect; the strain is difficult to develop resistance for the control object; the strain does not pollute the environment which is degraded by microorganism in the soil rapidly; the strain does not affect soil fertility and microbial flora; the strain does not generate bioconcentration phenomenon which is very safe for the ecology. The invention is a key enzyme inhibitor type of pesticide, which has no cross resistance with other insect resistant pesticides.

Description

(1) Technical field [0001] The present invention relates to a new bacterial strain that can prevent and treat phytopathogenic fungi and kill acarids——Streptomyces sp.S1-5 (Streptomyces sp. -5 The prepared emulsion that can be used to resist phytopathogenic fungi and kill acarids. (2) Background technology [0002] Biological pesticides are recognized as green pesticides and ideal pesticides in the 21st century because of their low toxicity, no residue, no pollution, and easy availability of raw materials. The development of new biological pesticides is undoubtedly an advanced behavior with both social and economic benefits. Acarids and phytopathogenic fungi are the main hazards that cause various economic crops such as vegetables, tea, fruit trees, and cotton. According to statistics, the area that needs to be controlled by mites alone in the country is no less than 100 million mu, causing annual output losses of 12% to 20%. In severe cases, it reaches more than 25%. The ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P7/02A01P7/04C12R1/465
Inventor 吴石金
Owner ZHEJIANG UNIV OF TECH
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