High-effective clean method for preparing soybean isoflavone aglycone

A technology of isoflavone aglycone and soybean, which is applied in the field of preparation of functional soybean isoflavones, to achieve the effect of accelerating the reaction speed, short production cycle, simple and easy to control

Active Publication Date: 2008-06-18
常熟紫金知识产权服务有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

[0012] In order to solve the deficiency of soybean isoflavone aglycone prepared by the existi

Method used

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  • High-effective clean method for preparing soybean isoflavone aglycone
  • High-effective clean method for preparing soybean isoflavone aglycone

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: the preparation of β-glucosidase:

[0032] The preparation of β-glucosidase in the present invention can be Thermotoga maritima (specific operation reference Yu Jiang, Qing Zhou, Kang Wu, Xiangqian Li, and Weilan Shao.A highly efficient method for liquid and solid cultivation of the anaerobic hyperthermophilic eubacterium Thermotogamaritima.FEMS Microbiol Lett, 2006, 259: 254-259) in a medium containing 1.0% glucose, 0.5g% yeast powder, 2.7% NaCl, filled with CO at a temperature of 80°C 2 and N 2 Cells were collected and broken after static culture for 8-10 hours under the condition, and the supernatant was collected by centrifugation, and the enzyme protein was precipitated with 65% saturated ammonium sulfate, and then dialyzed with 0.02M, pH5.5 phosphate buffer solution and freeze-dried to obtain β- Glucosidase.

Embodiment 2

[0034] The process shown in Figure 1, the specific operations are as follows:

[0035] (1) Grinding the mildew-free soybeans into 80-mesh soybean powder after removing impurities, and then extracting and removing fat with n-hexane to obtain defatted soybean powder;

[0036] (2) Dissolve the defatted soybean powder in pH 6.2 100mM sodium phosphate buffer solution at a material-to-water ratio of 1:4, heat to 90°C while stirring, add 800U / g Thermotoga maritima β-glucosidase, keep warm and Stir at low speed for 3 hours;

[0037] (3) The obtained enzymolysis solution was separated by centrifugation, and the precipitate was extracted with 10 times the volume of 80% ethanol at 80°C for 2 hours, then suction filtered to remove the residue, and the filtrate was further vacuum freeze-dried, and the obtained product was detected by HPLC. The composition of isoflavones is shown in Figure 3; HPLC analysis conditions: chromatographic column: Agilent HC-C 18 (4.6mm×250mm ID, 5μm); column t...

Embodiment 3

[0038] Embodiment 3: It is basically the same as Embodiment 1, except that the raw material used is soybean meal purchased from a domestic biotechnology company. The feed solution was prepared by dissolving soybean meal in pH 5 citric acid buffer solution with a feed-to-water ratio of 1:3.

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Abstract

The invention provides a clean and highly efficient preparation method of soybean isoflavone glycoside. The steps include dissolving degreasing soybean powder or soybean meal in water or buffer solution to prepare material solution; adding Beta-glucosidase in the material solution for the hydrolysis; adding ethanol in precipitates after the enzymatic hydrolysis for the extracting and the filtering to remove residues. A soybean isoflavone glycoside product is obtained after the vacuum concentration of the filtering solution. The invention changes an original two-step method of extracting soybean isoflavone and biologically transforming the soybean isoflavone into aglycone, uses the Beta-glucosidase with heat resistance in an extract process and directly uses the soybean meal or the degreasing soybean powder to prepare the soybean isoflavone glycoside. The soybean isoflavone in raw materials are being thermally extracted while being transformed. The inhibition effect of high concentrated initial substrates on the enzyme catalysis is avoided. The response speed is greatly increased. The enzyme hydrolysis efficiency is improved. Compared with the present enzymatic extraction, the invention has the advantages of short production cycle, simple operation, high yield, low cost and pollution-free property. And the obtained products are applied to food or drug safety.

Description

technical field [0001] The present invention relates to a preparation method of functional soybean isoflavones, in particular to an enzymatic preparation method of soybean isoflavone aglycone, and more specifically to provide a method suitable for industrial production of genistein and daidzein Methods. technical background [0002] In-depth research on the nutritional and health effects of soybeans and their products has found that soybeans contain not only rich protein and oil, but also many beneficial biologically active substances, such as soybean lecithin, soybean oligosaccharides, soybean saponins and soybean iso Flavonoids etc. Among these biologically active substances, soybean isoflavones have attracted more and more attention from society and academic circles because of their obvious biological activity, and have become a research hotspot of scientists from all over the world in recent years. Recent research results show that: soybean isoflavones have strong phys...

Claims

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Application Information

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IPC IPC(8): C12P17/06C12N9/24C12N9/42C12R1/01
Inventor 薛业敏于瑾瑾彭静静
Owner 常熟紫金知识产权服务有限公司
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