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Process for preparing diglyceride by enzyme method in organic medium system

A technology of diglyceride and enzymatic preparation, applied in biofuel, fermentation and other directions, can solve the problems of high viscosity of reaction substrate, slow reaction speed, lipase coating, etc., achieve easy separation, reduce negative effects, and improve enzyme effect of reactivity

Inactive Publication Date: 2008-07-23
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to propose a process for the enzymatic preparation of diglycerides in an organic medium system, which solves the problems of high viscosity of the reaction substrate, slow reaction speed, lipase is easily inactivated by substrate or by-product glycerin coating, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Be that the methyl alcohol of 1.5: 1 and 10g of jatropha oil and the tert-butanol based on oil volume 100% are packed into a stoppered Erlenmeyer flask and mixed uniformly, and placed in a reciprocating shaker that can automatically control the temperature and heated to 40 After ℃, add immobilized lipase Lipozyme TL with 5% oil mass to start the reaction, and filter and recover the immobilized lipase after 10 hours for reuse. The product is subjected to simple distillation to remove tert-butanol and methanol, and then 110°C vacuum distillation to remove residual tert-butanol and methanol, and then undergoes molecular distillation at 0.5Pa and 150°C to obtain fatty acid methyl ester and glyceride fractions respectively. Fatty acid methyl esters can be used as biodiesel, and glyceride fractions can be used as healthy oils and fats. The mass percentage of each component in the glyceride fraction is as follows: triglyceride 32.5%, diglyceride 53.4%, monoglyceride 13.8%, fre...

Embodiment 2

[0029] Ethanol and cottonseed oil (cottonseed oil 10g) with a molar ratio of 2:1, tert-butanol based on 120% of the oil volume, are packed into a stoppered Erlenmeyer flask and mixed evenly, and placed in a reciprocating shaker that can automatically control the temperature After heating to 60° C., 3% of the immobilized lipase Novozym 435 based on the oil mass was added to start the reaction, and after 6 hours, the immobilized lipase was recovered by filtration and reused. The product is subjected to simple distillation to remove tert-butanol and ethanol, and then 110°C vacuum distillation to remove residual tert-butanol and ethanol, and then undergoes molecular distillation at 0.5Pa and 150°C to obtain fatty acid ethyl ester and glyceride fractions respectively. Fatty acid ethyl esters can be used as biodiesel, and glyceride fractions can be used as healthy oils and fats. The mass percent content of each component in the glyceride fraction is as follows: triglyceride 22.1%, d...

Embodiment 3

[0031] Butanol and yeast fat (yeast fat 10g) with a molar ratio of 1:1, tert-butanol based on the fat volume of 60%, are packed into a stoppered Erlenmeyer flask and mixed evenly, and placed in a reciprocating shaker with automatic temperature control After heating to 35°C, 3% of the immobilized lipase Lipozyme RM based on the mass of the oil and 5% of the immobilized lipase Lipozyme TL based on the mass of the oil were added to start the reaction. After 15 hours, the immobilized lipase was recovered by filtration and reused. The product undergoes simple distillation to remove tert-butanol and butanol, and then undergoes vacuum distillation at 110°C to remove residual tert-butanol and butanol, and then carries out molecular distillation at 0.5 Pa and 150°C to obtain fatty acid butyl ester and glyceride fractions respectively. Fatty acid butyl esters can be used as biodiesel, and glyceride fractions can be used as healthy oils and fats. The mass percent content of each componen...

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PUM

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Abstract

The invention relates to an enzymatic process to prepare the diglyceride from an organic medium system, belonging to the vegetable oil synthesis field, which is characterized in that a short chain alcohol and lipid with the molar ratio of the alcohol ester being 0.5:1 to 2:1, an organic solvent taking over 20% to 300% of lipid volume, and an immobilization lipase with dry weight taking over 0.5% to 20% of the lipid mass are filled into a conical flask with a plug and are stirred evenly, the conical flask positioned in an automatic temperature controlled reciprocating shaker is heated up to 20 to 60 degree centigrade and reacted for 1 to 48 hours, then the oil material is transformed into the diglyceride and two secondary products the monoglyceride and the fatty acid short chain alcohol ester; the products are distilled to remove the organic solvent, the diglyceride is made after the reduced pressure distillation and the molecular distillation, and meanwhile the secondary product fatty acid short alcohol ester is produced. The enzymatic process to prepare the diglyceride from an organic medium system has the advantages of perfectly solving the problem of big viscosity of the reaction substrate during the process of producing diglyceride by means of the biological enzyme, effectively lowering the negative influence of the short chain alcohol on the enzyme reactivity, and significantly increasing the reaction efficiency.

Description

technical field [0001] The invention belongs to the field of bio-oil synthesis, and in particular relates to a process for preparing diglyceride by enzymatic method in an organic medium system, which uses an organic solvent as a reaction medium and lipase as a catalyst to produce diglyceride in an organic medium reaction system. Background technique [0002] Diacylglycerol (DG) is a structural lipid in which one fatty acid in triacylglycerol (TG) is replaced by a hydroxyl group. Diacylglycerol is a trace component of natural vegetable oil and an endogenous intermediate product of fat metabolism in the body. It is a food ingredient generally recognized as safe (GRAS). Diacylglycerol has many advantages such as safety, nutrition, good processing suitability, and high human compatibility. It is a kind of multifunctional additive and has been widely used in food, medicine, chemical (cosmetic) and other industries. In recent years, it has been found that dietary diglycerides can...

Claims

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Application Information

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IPC IPC(8): C12P7/62
CPCY02E50/13Y02E50/10
Inventor 刘德华杜伟里伟
Owner TSINGHUA UNIV
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