Method for preparing undenatured collagen from fish scale of fresh water fish

Abstract

The invention belongs to the agricultural natural product extraction field, in particular relating to a method for extracting unmodified collagen from freshwater fish scale. The method comprises the following steps that: (1) cleaned scale is added in distilled water and is stirred intermittently by a motor stirrer for 3 to 5 minutes till the surface of the scale has no attachment of pigment and fat, etc.; the scale is crushed to 40 to 50 meshes after natural air drying and low-temperature drying; (2) the materials of the step (1) are added into 8 to 10 percent of citric acid and malic acid solution according to a ratio of between 1 to 10 and 1 to 20 so as to carry out ice-water bath microwave treatment for 2 minutes and decalcification at a temperature of between 10 and 20 DEG C for 8 to 24 hours; then, the solution is filtered and the filtrate undergoes standing at a temperature lower than 10 DEG C for 5 to 10 hours so as to obtain calcium citrate precipitate; (3) according to a ratio of between 1 to 5 and 1 to 20, 1 percent malic acid or citric acid solution (containing protease) is added into the de-calcification scale obtained after the treatment of step (2), and is stirred and extracted 2 to 4 times at a temperature of between 0 and 20 DEG C with each time lasting 12 to 48 hours; then, centrifugation is carried out, and supernatant is taken out; NaCl is added till final concentration reach 0.7 to 0.90 mol / L, and then salting out is carried out over night; centrifugation and deposition are carried out, and the obtained substance is dissolved in 0.50mol / L acetic acid; then, salting out and dissolving operations are repeated one more time, and the precipitate undergoes dialysis so as to obtain the product of the invention through freeze drying.

Description

technical field [0001] The invention belongs to the field of chemical extraction of agricultural natural products, in particular to a method for preparing undenatured collagen from fish scales. Background technique [0002] Collagen protein is an important functional protein, which is closely related to cell proliferation, differentiation, exercise, immunity, joint lubrication, wound healing and so on. Due to the special functions of collagen, its extracts have been widely used in medicine, food, daily chemical industry, biosynthesis and other industrial fields, such as medical capsules, surgical materials, edible gelatin, photographic gelatin, cosmetics, etc. The raw materials of collagen products currently produced mainly come from the skin, bone and tendons of terrestrial mammals such as pigs and cattle. Due to the deterioration of the global ecological environment in recent years, many epidemics such as mad cow disease and foot-and-mouth disease have occurred, making the...

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Problems solved by technology

The literature of Nagai et al. reported a method for extracting natural collagen from seawater fish scales, but in the extraction process, sodium hydroxide soaking was also used to remove impurities, Tris-HCl and EDTA decalcification, and the three wastes were seriously polluted in the production process. The treatment process only needs 7 days, the extraction cycle is long, and it is difficult to realize industrialization; although the patent CN1597741 relates to a preparation method of undenatured natural fish collagen, fish skin is used as raw material, soaked in ether or non-ionic surfactant solution mixed with water 5-24h, filtered and stirred with EDTA solution for 24-48h

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