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Method for extracting amber acid in fermentation liquor by anion resin adsorption

An anion resin, adsorption and extraction technology, applied in the direction of fermentation, ammonium sulfate, separation/purification of carboxylic acid compounds, etc., to achieve the effects of shortening the process flow, improving product economic benefits, and reducing environmental protection treatment costs

Inactive Publication Date: 2009-01-21
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] Aiming at the existing problems in the extraction of fermented succinic acid, the present invention proposes a method for extracting succinic acid by anion resin adsorption from the perspective of element recycling and based on the ion exchange method

Method used

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  • Method for extracting amber acid in fermentation liquor by anion resin adsorption
  • Method for extracting amber acid in fermentation liquor by anion resin adsorption
  • Method for extracting amber acid in fermentation liquor by anion resin adsorption

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Experimental conditions: The fermentation broth used in this experiment was fermented with corn syrup as raw material, and MgCO was used in the fermentation process 3 Adjust pH.

[0031] The above-mentioned fermentation liquid was heated to 80° C., and after adding 0.1% powdered activated carbon by weight of the fermentation liquid for decolorization, the bacteria residue was removed by centrifugation at 8000 r / min for 10 min to obtain the fermentation supernatant.

[0032] The resin used is shown in Table 1. At 25°C, add 2g of wet resin and 20mL of 40g / L succinic acid fermentation broth into a 250mL Erlenmeyer flask, place the Erlenmeyer flask in a constant temperature shaker, and exchange it at 200r / min for 24h. When the concentration of succinic acid does not change any more, it means that the resin has reached separation saturation. Then measure the concentration of succinic acid in the solution, and calculate the ion exchange capacity of succinic acid by subtracti...

Embodiment 2

[0038] The above-mentioned magnesium succinate fermentation supernatant was put on the column for adsorption, and the flow rate of the upper column was 2.9h -1. Then wash away impurities with water 1 to 2 times the weight of the fermentation broth, elute with 1mol / L, 2mol / L, and 3mol / L hydrochloric acid, respectively, at a flow rate of 1.8h -1 、2.9h -1 、4.5h -1 elute down. Its experimental result is as table 3 (elution flow rate 1.8h -1 Different hydrochloric acid concentrations to the elution result of anion exchange resin adsorption succinic acid) and Table 4 (hydrochloric acid concentration 2mol / L different elution flow rates to anion exchange resin adsorption succinic acid elution result).

[0039] table 3

[0040]

[0041] The results in the above table show that the elution flow rate is 1.8h -1 When using 2mol / L hydrochloric acid elution effect is better.

[0042] Table 4

[0043]

[0044] It can be seen from the above table that the elution flow rate is co...

Embodiment 3-8

[0046] Adopt the process condition of embodiment 1 and embodiment 2, respectively with corn starch hydrolysis syrup, tapioca starch hydrolysis syrup, industrial sugar waste molasses (such as sugarcane molasses), corn stalk hydrolysis syrup, Jerusalem artichoke hydrolysis syrup, sweet sorghum stalk syrup Extract the fermentation broth as the raw material, and finally concentrate and crystallize the eluate to obtain the succinic acid product, use MgCO 3 When the pH of the fermentation process is adjusted by buffering, the effluent from the column can be prepared by the ammonium brine bicarbonate method to prepare magnesium carbonate, which is recovered and used to adjust the pH of the fermentation process.

[0047] The yields of each step of extracting succinic acid in various raw material fermentation broths by anion resin adsorption are shown in Table 5.

[0048] table 5

[0049]

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Abstract

A method for sorption extraction of succinic acid in fermentation broth by an anion resin belongs to the biochemical technical field. The method comprises the following steps that succinic acid fermentation broth undergoes decoloring treatment by heated active carbon; fungus residue is eliminated through centrifugal filtration or microfiltration membrane filtration; the obtained fermentation clear solution is adsorbed by an alkaline anion resin column, and impurity is flushed away by water after adsorption saturation and then is eluted by hydrochloric acid solution or sulfuric acid solution, thereby obtaining succinic acid solution; the succinic acid solution undergoes depressurized concentration and crystallization so as to obtain a succinic acid product; moreover, MgCl2 and residual sugar in effluent after anion resin column adsorption of the fermentation broth is reclaimed; the MgCl2 in the effluent generated during anion resin adsorption is passed through CO2 or (Na)2CO3 so as to prepare magnesium carbonate which is reclaimed and used in buffering and adjusting pH during fermentation; and sugar in filtrate is reclaimed and used in fermentation ingredients for further fermentation. The method can realize recovery and reuse of materials during operation, and substantially reduces material consumption during extraction; moreover, the method reduces the treatment cost of waste water for environmental protection, and increases the economic benefit of products.

Description

technical field [0001] The invention relates to an ion exchange method, which is mainly a method for extracting succinic acid in fermentation broth by adsorption of anion resin, and belongs to the technical field of biochemical industry. Background technique [0002] Succinic acid is an important C4 platform compound, and it is an important bulk chemical and specialty chemical such as 1,4-butanediol, tetrahydrofuran, γ-butyrolactone, PBT resin, N-methylpyrrolidone, adipic acid, etc. It is also an important food additive and feed additive. Traditional methods for chemically synthesizing succinic acid mainly include catalytic hydrogenation of maleic acid, paraffin oxidation and electrochemical synthesis of maleic acid. Due to environmental pollution, rising cost of raw materials and other reasons, the research on the production of succinic acid by microbial fermentation of renewable raw materials has been paid more and more attention as the petrochemical resources are decreas...

Claims

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Application Information

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IPC IPC(8): C07C55/10C07C51/47C01C1/24C12P7/46
Inventor 孙志浩郑璞倪晔董晋军
Owner JIANGNAN UNIV
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