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Percolation extraction method for raffinose

A technology of percolation extraction and raffinose, which is applied in the field of chemical engineering, can solve the problems of low yield of raffinose product, high viscosity of crystallization solution, decreased crystallization rate, etc. Effect

Active Publication Date: 2009-02-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent application 200710014172.4 discloses a method for extracting raffinose from cottonseed meal, and the yield of raffinose is also very low
In this invention, pure water is used as the crystallization solvent, and the mass percentage concentration of the initial raffinose is 60%. It takes as much as 50 hours for the cooling time alone, and the yield of final raffinose is also low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: prepare the percolation extract of raffinose

[0042] Degreased cottonseed meal (wherein the mass percentage of raffinose is 3.9%) is sieved through a sieve of 1.8 mm, weighs 1000 grams of cottonseed meal and puts it into a percolation column (Φ5.6cm×70cm), and it is piled up after filling evenly The volume is about 1300 milliliters, and then 780 milliliters of ethanol aqueous solution with a volume percentage concentration of 75% is added to the percolation column, soaked at 55°C for 30 minutes to make it fully swell. Under the heat preservation state, open the outlet valve of the percolation column, and continuously add 75% ethanol aqueous solution from the top of the percolation column at the same time, control the flow rate at 12 ml / min until 4200 ml of percolation extract is collected. After analysis of the percolation extract, the solid content (solid content refers to the percentage of the solution mass before the solution is dried to a constant wei...

Embodiment 2

[0044] Embodiment 2: prepare the percolation extract of raffinose

[0045] Degreased cottonseed meal (wherein the mass percentage of raffinose is 3.9%) is sieved through a 1.8mm sieve, and 3000 grams of cottonseed meal are weighed and packed into three percolation columns (Φ5.6cm×70cm) of the same specification respectively, The average value of the bulk volume of its defatted cottonseed meal after packing evenly is about 1260 milliliters. First, add about 760 milliliters of 75% ethanol aqueous solution into the percolation column, and soak at 55° C. for 30 minutes to fully swell the defatted cottonseed meal. Under the heat preservation state, open the outlet valve of the first percolation column, and continuously add ethanol aqueous solution with a volume percentage concentration of 75% from the top of the percolation column, and control the flow rate at 8 ml / min until the collected percolation extract reaches 4000ml only.

[0046] Add deionized water from the top of the pe...

Embodiment 3

[0050] Embodiment 3: Preparation of high-purity raffinose

[0051] (a) Concentrate the 1900 milliliters of percolation extract prepared by the method of Example 1 to 1 / 10 of the original volume, add water to prepare a solution (wherein the solid content is 10%), and add 5% hydrochloric acid for adjustment pH to 5.

[0052] About 230 grams of granular activated carbon (adsorbent A) was loaded into an adsorption column with a size of Φ5.6cm×40cm, and the column bed volume BV was 980mL.

[0053] The above-mentioned pH-adjusted aqueous solution was passed through a fixed-bed adsorption column equipped with granular activated carbon at a flow rate of 1.0 BV / h, and the temperature was controlled at 40°C. Collect the effluent (i.e. the decolorized solution) of the loading process of the fixed-bed adsorption column until the collected decolorized solution is 10BV, and its color is lighter (the color value is less than 10% of the color value of the sample aqueous solution). On a dry ...

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PUM

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Abstract

The present invention discloses a percolation extraction method of raffinose. Utilizing extraction solvent for extraction, the method includes the following processes: degreased cotton seed meal is used as material, methanol aqueous solution or ethanol aqueous solution, the volume percentage concentration of which is 70 percent to 85 percent, is used as the extraction solvent, which is added into a percolation column containing the degreased cotton seed meal under the temperature of 50 DEG C to 70 DEG C, and after the degreased cotton seed meal is sufficiently swelled, the extraction solvent flows through the percolation column in order to collect percolated extract at an outlet. In the percolated extract, the extraction rate of the raffinose is more than 95 percent. The method has the advantages of high extraction rate, little solvent consumption, low production cost, easy industrialized production, and the like.

Description

technical field [0001] The invention relates to a method for extracting raffinose, belonging to the technical field of chemical engineering. Background technique [0002] Raffinose, also known as Melitriose and raffinose, is composed of galactose, glucose and fructose, and is an oligosaccharide and a non-reducing sugar. Research has found that raffinose is an excellent nutrient source for beneficial bacteria such as Bifidobacterium and Lactobacillus acidophilus in the human intestinal tract, which can promote their proliferation, thereby inhibiting the growth of spoilage bacteria in the intestinal tract and reducing the formation of toxic fermentation products . Bifidobacteria ferment raffinose to produce a large amount of short-chain fatty acids, which can stimulate intestinal peristalsis, increase stool moisture and maintain a certain osmotic pressure, thereby preventing constipation. In addition, raffinose can improve the digestive function of the human body, promote th...

Claims

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Application Information

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IPC IPC(8): C07H3/06C13K13/00B01D11/04
Inventor 吴彩娟苏宝根鲍宗必朱魏任其龙
Owner ZHEJIANG UNIV
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