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Zymohydrolysis lactoprotein ferrous complex compound microcapsule iron supplement agent and preparation thereof

A milk protein and complex technology, applied in the biological field, can solve the problems of low absorption utilization rate, toxic and side effects, etc., and achieve the effects of improving absorption utilization rate, no toxic and side effects, and preventing and treating iron deficiency anemia.

Inactive Publication Date: 2011-10-05
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Iron supplements currently used in my country have defects such as low absorption and utilization rate and certain side effects. It is necessary to find substances that promote iron absorption, improve iron utilization rate, and provide consumers with a good taste, safe and non-toxic supplement. Iron

Method used

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  • Zymohydrolysis lactoprotein ferrous complex compound microcapsule iron supplement agent and preparation thereof
  • Zymohydrolysis lactoprotein ferrous complex compound microcapsule iron supplement agent and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Casein was purchased from Northwest Biotechnology Company; alkaline protease was purchased from Novozymes Biotechnology Co., Ltd.; sodium alginate was purchased from Beijing Aoboxing Biotechnology Co., Ltd. Whey protein was purchased from Beijing Yinhe Road Company.

[0042]Take 0.6 kg of casein, make it into a 6% (w / v) protein solution, add 0.6 g of alkaline protease, adjust the enzymolysis temperature to 42°C, enzymolysis time is 7 hours, and the pH value is 8.0 for enzymolysis. After the end of the enzymolysis, Inactivate the enzyme at 90°C for 10 minutes, and perform ultrafiltration with an ultrafiltration device (a blended ultrafiltration membrane with a molecular weight cut-off of 10 kDa) to obtain a relatively pure milk protein hydrolyzate, which is cooled to room temperature for later use.

[0043] Dissolve the hydrolyzate of milk protein with distilled water, according to the bioactive peptide and FeSO 4 The mass ratio (calculated by Fe) is 2:1, the reaction p...

Embodiment 2

[0046] Crude cheese was from Xinjiang; alkaline protease was purchased from Beijing Fangshan Enzyme Preparation Factory; chitosan was purchased from Beijing Aoboxing Biotechnology Co., Ltd.; whey protein was purchased from Beijing Yinhe Road Company.

[0047] Take 0.6kg of crude cheese and 0.1kg of whey protein, make it into a 3% (w / v) protein solution, add 0.25g of alkaline protease, adjust the enzymatic hydrolysis temperature to 55°C, enzymatic hydrolysis time for 9h, and pH value 7.0 for enzymatic After enzymatic hydrolysis, inactivate the enzyme at 60°C for 30 minutes, then cool to room temperature for later use.

[0048] The milk protein hydrolyzate solution was taken, and the mass ratio of bioactive peptide and ferrous lactate (calculated as Fe) was 4:1, the reaction pH value was 4.0, the reaction temperature was 40°C, and the complexation time was 55min. A solution containing milk protein bioactive peptide ferrous complex is prepared. The complexation reaction is carri...

Embodiment 3

[0051] Casein: from Northwest Biotechnology Company; alkaline protease was purchased from Novozymes Biological Co., Ltd.; modified starch was purchased from National Starch Company; whey protein was purchased from Beijing Yinhe Road Company.

[0052] Take 0.6 kg of casein, make it into a 9% (w / v) protein solution, add 1.42 grams of alkaline protease, adjust the enzymolysis temperature to 25° C., enzymolysis time of 4 hours, and pH value 9.0 for enzymolysis. After the enzymolysis, Inactivate the enzyme at 75°C for 15 minutes, cool to room temperature, and set aside.

[0053] Use ultrafiltration equipment (ceramic ultrafiltration membrane with a molecular weight cut-off of 10kDa) for ultrafiltration, and use Sephadex G-25 for gel filtration chromatography to obtain a solution of higher purity milk protein hydrolyzate, and cool to room temperature spare.

[0054] Take the above-mentioned enzymolyzed milk protein solution (after deactivating the enzyme) and dissolve it. The mass ...

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Abstract

The invention relates to an enzymolytic lactoprotein ferrous complex, a microcapsule iron supplement and a preparation method thereof. In the preparation method, ferrous salt is complexed with an enzymolysis product of lactoprotein, which can improve solubility of iron salt under an alkalescent intestinal condition, improves absorptivity and availability of iron and ensures a safe and pollution-free preparation process; a proper coating material is adopted for microencapsulating the enzymolytic lactoprotein ferrous complex to prepare the microcapsule iron supplement agent of the enzymolytic lactoprotein ferrous complex; the iron supplement agent can mask self-odor of the ferrous salt, prevent the ferrous iron from oxidation, gradually release Fe<2> under the alkalescent intestinal condition to ensure high biological absorptivity and biological availability of the Fe<2>, and has no toxic and side effect; and the enzymolytic lactoprotein can prevent and treat iron-deficiency anemia, and has physiological functions of resisting oxidation, adjusting immunity, lowering blood pressure and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a milk protein bioactive peptide ferrous complex, its microcapsules, their preparation method and biological application. Background technique [0002] Iron is one of the essential micronutrients for the human body and plays an important role in the metabolism of the body. At present, nutritional iron deficiency is a common nutritional problem in developing countries, especially in children and pregnant women. The iron in food mainly exists in the form of ferric iron, while the iron used by the human body is mainly ferrous iron, so the absorption and utilization rate of iron in food is low. [0003] In addition, the absorption of iron is also related to the state of iron, its solubility, and other ingredients in the food. For example, foods containing a large amount of phosphate or oxalate interfere with the absorption of iron, while Vc, cysteine, etc. Sexual substances are conduci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K4/12C07K1/34C07K1/18C07K1/16C12P21/06A61K38/03A61K9/50A61P3/02A61P39/06A61P37/02A61P9/12
Inventor 毛学英许成才王磊徐世平任发政
Owner CHINA AGRI UNIV
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