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Local surface plasma resonance reinforced biochemical detector

A plasma resonance and biochemical detector technology, applied in the field of biochemical detectors, can solve the problems of low sensitivity, complex system, long cycle, etc., and achieve the effects of high detection sensitivity, high degree of intelligence, and convenient operation

Inactive Publication Date: 2009-08-26
INST OF OPTICS & ELECTRONICS - CHINESE ACAD OF SCI
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is: to overcome the shortcomings of the existing biochemical sensing technology such as complex system, high cost, long cycle, and low sensitivity, and utilize localized surface plasmon technology (LSRP) to form a high-sensitivity, label-free, Biochemical detector

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  • Local surface plasma resonance reinforced biochemical detector

Examples

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Embodiment 1

[0027] In this embodiment, the local surface plasmon resonance enhanced biochemical detector of the present invention is used to detect biological toxins. The light source in the selected light source system is a halogen lamp or a sodium lamp with a spectral range of 300 to 1000 nm. After beam shaping system expansion and shaping, the light emitted from the beam is irradiated in the sensing system using K9 glass as the substrate. Probe chip 1. like image 3 As shown, in the sensing system, the detection chip 1 is installed on the detection chip clamping mechanism 2; the spectrum analysis system uses the USB4000 type spectrum analyzer of Ocean Optics with a spectral resolution of 1.5nm and a wavelength reproducibility of less than ±0.2nm. , the light passes through the detection chip and then is detected by the back-end spectrum analyzer; the collected data communicates with the computer system through the USB2.0 interface, and the data processing software can include user int...

Embodiment 2

[0029] This embodiment adopts the local surface plasmon resonance enhanced biochemical detector of the present invention for testing bacteria. In the light source system, a mercury lamp with a spectral range of 200-800 nm is used as the light source, the light source is separate and integrated, and optical fiber is used to guide light; the incident light is irradiated on the PDMS-based detection chip with high sensitivity to bacteria in the sensing system; Chip photos such as Figure 5 As shown, there is a microfluidic channel prepared by a micromechanical method, and the bacteria to be tested are passed into the sample injection and recovery system to realize on-line detection. The spectrum analysis system uses a spectrum analyzer with a spectral resolution of 2nm and a wavelength reproducibility of less than ±0.3nm to test its spectrum; the parallel port is used for data communication with the computer system, and the data processing software installed on the computer is use...

Embodiment 3

[0031] In this example, the localized surface plasmon resonance enhanced biochemical detector of the present invention is used to test proteins. The light source system uses LEDs with a spectral range of 600-1500nm as the light source; the sensing system uses fused silica glass as the substrate for protein detection chips; the spectral analysis system uses an integrated LSPR detector for detection and analysis, and uses a USB port and a computer The system performs communication and data processing. The overall size of the host system is less than 300×150×120mm 3 ; The prototype provides USB2.0 interface; the weight is less than 3kg, suitable for field use. A large number of experiments were carried out in the laboratory to collect spectral data in advance, and a standard database of protein molecules of different types and concentrations was established, which can facilitate the comparison of detection results and signal extraction, and the instrument has high operability.

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Abstract

A local surface plasma resonance reinforced biochemical detector is characterized in that the detector comprises four parts as follows: a light source system, a sensing system, a spectral analysis system and a data processing system, wherein, the sensing system thereon is equipped with a detection chip, the light source system provides light source, the sensing system and the spectral analysis system are located in the output optical path behind the light source in sequence and are deposited perpendicularly to an optical axis, the light emitted from the light source irradiates the detection chip of the sensing system, the spectral analysis system is located behind the sensing system and detects the light rays passing through the detection chip, and the collected data information is transmitted to the data processing system for data processing. The detector achieves the quick identification of the detected object with high sensitivity and is suitable for requirements on external field test. The device of the invention has a simple structure and low cost, is convenient to be carried and convenient to be popularized, and can satisfy the requirements on biochemical detection in fields, public places and hospitals.

Description

technical field [0001] The invention belongs to the field of biochemical detection, and relates to a biochemical detector utilizing localized surface plasmon resonance enhancement (LSPR) technology. Background technique [0002] Sensing technology is one of the three pillars of modern information technology and plays an important role in national security, scientific experiments, medical care, and environmental monitoring. Biochemical sensing technology is an important branch of sensing technology, which is related to public security, virus, bacteria detection, clinical medicine, environmental detection and other fields closely related to people's lives. Traditional biochemical sensing technology usually requires labeling, of which 90% of the workload is used for labeling, mainly using radioisotopes, enzymes or fluorescence as markers, which have poor safety and stability. nanomolar scale), the process is cumbersome and the efficiency is low. It cannot meet the needs of fa...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N21/55G01N21/552
Inventor 邓启凌杜春雷史立芳杨欢
Owner INST OF OPTICS & ELECTRONICS - CHINESE ACAD OF SCI
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