Preparation method of high purity bulleyaconitine A

Abstract

The invention relates to a preparation method of high purity bulleyaconitine A. The preparation method comprises the following steps: root of radix aconiti feri of Yunnan is crushed, and acid alcoholic solvent or alcohol water solution is used for diacolation, and supersound or thermal refluxing extraction. The extracting solution is decompressed and concentrated to be non-alcoholic at the temperature of 80 to 90 DEG C, cooled down to room temperature, to ensure the relative density to be 1.00 to 1.30; standing is performed for 10 to 20 hours; the extracting solution is filtered; the pH is adjusted to 6.0 to 8.0 with basifier; and then the extracting solution is extracted with ethyl acetate or chloroform. The ethyl acetate or chloroform liquid is contracted, column chromatography is performed to the obtained paste, silica gel or neutral alumina are used as fillers, any one of solvent vapor-acetone-diethylamine, ligarine-acetone-diethylamine or cyclohexane-acetone-diethylamine is used for eluation, the bulleyaconitine A in the collected liquid is constantly checked during the thin layer chromatography, the collected liquid containing the bulleyaconitine A is merged, the solvent is concentrated, carbinol or ethanol is dissolved, filtering is performed, crystallization is performed at room temperature, filtering is performed, the crystal is repeatedly rinsed with carbinol or ethanol, and the final product is obtained. The purity of the obtained product is high, the production cost is low, and the toxicity of the used organic solvent is low.

Description

technical field [0001] The invention relates to a method for extracting high-purity aconitin from the root tuber of a kind of natural plant Aconitum aconitifolia. Background technique [0002] Bullyaconitione A (Bulleyaconitione A) was also known as Dianxi doulaine A or dragon head aconitine A, molecular formula C 35 h 49 o 10 N, molecular weight 643.77, is a colorless prismatic crystal. Soluble in ether, alcohol, acid water, insoluble in water, has strong analgesic and obvious anti-inflammatory effects. Experiments have proved that the analgesic effect of this product is central and closely related to the level of serotonin in the brain. Non-addictive. Its anti-inflammatory effect does not pass through the adrenal system, but is related to the suppression of PG levels; this product also has antipyretic and local anesthetic effects. After taking the medicine, there were no abnormal changes in the patients' ECG, EEG, liver and kidney function and urine routine examinati...

AI Technical Summary

Problems solved by technology

There are problems such as low content, high production cost, high production safety risk, etc., and it is difficult to industrialize large-scale production

In the patent of CN1054976A, the method of semi-synthesizing aconitine from aconitine is disclosed. In actual operation, it is difficult to separate and purify the impurities produced by the reaction, and it is difficult to separate and obtain qualified starting raw material aconitine, which leads to high production cost and also Difficult to industrialize large-scale production

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