Humanization active forging bone and preparation method thereof
A calcined bone, humanized technology, applied in medical science, prosthesis, etc., to achieve moderate degradation speed, convenient clinical operation, and favorable effects of adhesion and growth
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Embodiment 1
[0023] 2. Calcination: Put the dried bone fragments into a corundum crucible and calcinate in a muffle furnace; raise the temperature to 800°C, continue calcination at a constant temperature for 4 hours, lower the temperature in the furnace to room temperature, and take out the calcined bone;
[0024] 3. Surface treatment: After the calcined bone is mechanically crushed, the calcined bone particles with a particle size of 100-200 μm are selected with a sieve, placed in 2 mmol / ml RGD polypeptide solution, soaked for 1 hour after negative pressure suction; Sterilize after drying.
[0025] 4. Preparation of bone marrow mesenchymal stem cell culture medium: its components include by volume percentage: commercial minimum essential culture medium DMEM 67.5%, commercial culture medium F12 22.5%, fetal bovine serum 10%, insulin 50ng / ml, hydrogenated can Pine 500ng / ml, adenine 0.05mM, basic fibroblast growth factor 4ng / ml, transferrin 8μg / ml, vitamin C 40μg / ml.
[0026] 5. Cell planti...
Embodiment 2
[0031] 2. Calcination: put the dried bone fragments into a corundum crucible, and calcinate in a muffle furnace; raise the temperature to 600°C, continue to calcine at a constant temperature for 3 hours, lower the temperature in the furnace to room temperature, and then raise the temperature to 1000°C, and keep the temperature constant Calcined for 3 hours and lowered to room temperature, the calcined bone was taken out, mechanically pulverized, and bone particles with a particle size of 1-2 mm were sorted out with a sieve.
[0032] 3. Surface treatment: In order to make the planting cells easier to attach, place the calcined bone particles in 2 mg / ml collagen solution for negative pressure suction, soak for 1 hour; then vacuum dry and then sterilize.
[0033] 4. Preparation of osteoblast culture medium: its components include by volume percentage: commercial minimum essential culture medium DMEM 67.5%, commercial culture medium F12 22.5%, fetal bovine serum 5%, insulin 10ng / ml...
Embodiment 3
[0039] 2. Calcination: put the dried bone fragments into a corundum crucible, and calcinate in a muffle furnace; heat up to 700°C, and calcine at a constant temperature for 4 hours, then lower the temperature in the furnace to room temperature, and then heat up to 1100°C, and calcine at a constant temperature After 3 hours, it was lowered to room temperature, and the calcined bone was taken out, crushed mechanically, and then separated into bone particles with a particle size of 4-5 mm by a sieve.
[0040] 3. Surface treatment: In order to make it easier for the planting cells to attach, the calcined bone particles are placed in 2 mmol / ml RGD polypeptide solution and vacuum-suctioned, soaked for 1 hour; then dried at room temperature and then sterilized.
[0041] 4. Preparation of bone marrow mesenchymal stem cell culture medium: its components include by volume percentage: commercial minimum essential culture medium DMEM 67.5%, commercial culture medium F12 22.5%, fetal bovine...
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