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A method for preparing l-aspartic acid

A technology of aspartic acid and aspartic acid, which is applied in the field of bioengineering, can solve the problems affecting the sustainable production of L-aspartic acid and the high production cost, and achieves the benefits of sustainable production and saving Production cost, the effect of a wide range of applications

Inactive Publication Date: 2011-12-28
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] And the used fumaric acid raw material in the technology of prior art all originates from maleic anhydride isomerization method preparation, is petroleum-based product, and along with the increasingly scarce of fossil resource, its production cost is on the high side day by day; Depletion will also affect the sustainable production of L-aspartic acid

Method used

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  • A method for preparing l-aspartic acid
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  • A method for preparing l-aspartic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Fumaric acid fermentation broth:

[0029] Rhizopus oryzae (Rhizopus oryzae ME-F12) strain was used, and the fermentation medium formula was: glucose 130g / L, urea 0.06g / L, KH 2 PO 4 0.6g / L, MgSO 4 ·7H 2 O 0.5g / L, ZnSO 4 ·7H 2 O 0.0176g / L, FeSO 4 ·7H 2 O 0.000498g / L, CaCO 3 It is 60% of the glucose concentration and the pH is 5.5. For fermentation conditions, see literature (Chinese Journal of Biotechnology, 2008, 28(4): 59-64). After the fermentation ended, the concentration of fumaric acid (calcium salt) in the fermentation broth was 66.5g / L, the concentration of residual glucose was 7.8g / L, and the concentration of fumaric acid in the clear liquid was 4.6g / L.

[0030]Bacteria: Escherichia coli (E.coli ATCC 11303)

[0031] (1) Preparation of enzyme-producing medium for producing aspartase microorganisms

[0032] Add nutrients to the supernatant of fumaric acid fermentation broth: beef extract 2g / L, peptone 6g / L, magnesium sulfate 0.25g / L, potassium dihydroge...

Embodiment 2

[0043] The present embodiment is the same as the method of embodiment 1, but the conditions are changed.

[0044] Fumaric acid fermentation broth:

[0045] Rhizopus oryzae (Rhizopus oryzae ME-F12) strain was used, and the fermentation medium formula was: glucose 130g / L, urea 0.06g / L, KH 2 PO 4 0.6g / L, MgSO 4 ·7H 2 O 0.5g / L, ZnSO 4 ·7H 2 O 0.0176g / L, FeSO 4 ·7H 2 O 0.000498g / L, CaCO 3 It is 60% of the glucose concentration and the pH is 5.5. For fermentation conditions, see literature (Chinese Journal of Biotechnology, 2008, 28(4): 59-64). After the fermentation, the concentration of fumaric acid (calcium salt) in the fermentation broth was 62g / L, the concentration of residual glucose was 10.3g / L, and the concentration of fumaric acid in the clear liquid was 4.2g / L.

[0046] Bacteria: Escherichia coli (E.coli ATCC 11303)

[0047] (1) Preparation of enzyme-producing medium for producing aspartase microorganisms

[0048] Nutrients are added to the supernatant of the ...

Embodiment 3

[0059] The present embodiment is the same as the method, strain, and source of fumaric acid fermentation broth in Embodiment 1, but the conditions are changed.

[0060] (1) Preparation of enzyme-producing medium for producing aspartase microorganisms

[0061] Nutrients are added in the supernatant of fumaric acid fermentation broth: dry corn powder 8g / L, beef extract 4g / L, magnesium sulfate 0.20g / L, potassium dihydrogen sulfate 0.4g / L, pH 7.0 (adjusted with ammonia water);

[0062] (2) Culture of Enriched Aspartase Cells

[0063] Take a ring of well-grown slant seeds in a 250mL shake flask containing 30mL of the above-mentioned enzyme-producing medium, place it on a shaker with a temperature of 37°C and a rotation speed of 180r / min, and culture it with shaking for 14 hours to obtain a seed liquid; 10% (v / v) of the inoculum was transferred to the enzyme-producing medium for expansion cultivation at a temperature of 37° C., ventilation of 0.7 vvm, stirring speed of 150 r / min, c...

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Abstract

The invention discloses a method for preparing aspartase and L-aspartic acid by using fumaric acid fermentation liquid. The method comprises two parts, one is to cultivate Escherichia coli to produce aspartase in the supernatant part of the fumaric acid fermentation broth, and the other is to use the solid precipitation part of the fermentation broth to react calcium fumarate with ammonium sulfate to generate ammonium fumarate, After concentration, L-aspartic acid is generated under the action of aspartase. This method realizes the production of L-aspartic acid by the glycosyl route, realizes the full utilization of fumaric acid fermentation broth and achieves the goal of energy saving and emission reduction, effectively reduces the production cost of L-aspartic acid, reduces The pollution to the environment makes L-aspartic acid obtain sustainable production, and has a wide prospect of industrialized production.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for preparing L-aspartic acid from a fumaric acid fermentation liquid. Background technique [0002] L-Aspartic acid (L-Aspartic acid, abbreviated as L-Asp), chemical name α-aminosuccinic acid. As a common acidic amino acid, L-aspartic acid has a very wide range of uses in medicine, food, beauty, chemical industry, materials, etc. Polyaspartic acid, a new type of biodegradable polymer material with aspartic acid as the only monomer, is more and more widely used; aspartic acid is also one of the two raw materials of the new sweetener aspartame, and it It is also the synthetic precursor of L-lysine and purine and pyrimidine bases, as K + , Mg 2+ Ionophores deliver electrolytes to the myocardium for improved myocardial function. Therefore, the market application prospect of aspartic acid is more and more optimistic. [0003] In the prior art, the pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/20C12R1/19
Inventor 黄和李霜盛晓燕付永前高振
Owner NANJING TECH UNIV
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