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Alcohol and succinic acid production method by fermenting cellulosic raw material

A technology for cellulose and succinic acid, which is applied in the field of producing ethanol and succinic acid by using a cellulose-based raw material fermentation method, can solve the problems of unutilized and the like, achieves low production cost, wide source of raw materials, and improved fermentation production efficiency. Effect

Active Publication Date: 2009-12-30
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large amount of cellulose-based agricultural and forestry wastes such as crop straws, tree branches, forest processing leftovers, sawdust and shavings will be produced in agricultural and forestry production. The total amount of straws in my country is about 600-700 million tons per year, and corn straw alone is about 1.23 100 million tons, branches, sawdust, sawdust, and truncations produced in the process of logging and wood processing each year amount to more than 39.3 million cubic meters. These are renewable resources with great potential. , mostly unused

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Preparation of sugar solution with corn stalks as raw material

[0024] Using corn stalks as raw materials to prepare sugar liquid comprises the following steps:

[0025] (1) Take 100 kg of corn stalks, remove the leaves and roots by hand, and process them with a straw feed kneading machine and a chip grinder. The aperture of the bottom discharge screen is Φ5, and the corn stalk powdery material is obtained.

[0026] (2) Using a cylindrical reactor with a volume of 0.8 cubic meters and a rotation speed of 1 rpm, 100 kilograms of crushed corn stalks were added to it, and 600 kilograms of dilute alkali solution with a concentration of 1.8% sodium hydroxide was added. Heating to 120°C, keeping the temperature for 1 hour, cooling down, and releasing the material.

[0027] (3) The material after the alkali treatment is squeezed dry with a screw squeezer, and the solid phase is washed with hot water until the pH value of the material is less than 9.

[0028] (4) J...

Embodiment 2

[0031] The establishment of embodiment 2 ethanol fermentation system

[0032] The establishment of ethanol fermentation system comprises the following steps:

[0033] (1) Plate culture: the plate medium consists of 3% glucose, 0.5% yeast extract, 0.5% peptone, and 2% agar. Alcoholic yeast strains were inoculated into the medium, and cultured at 33°C for 28 hours.

[0034] (2) Shake flask seed culture: the composition of the shake flask seed medium is glucose 3%, yeast extract 0.5%, peptone 0.15%, corn steep liquor 0.5%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.06%, and tap water preparation. Inoculate the alcoholic yeast strain cultured on the plate into the medium, and culture at 33° C. for 32 hours, with the rotating speed of the shaker at 200 rpm.

[0035] (3) seed tank seed culture: medium composition is the concentrated sugar solution that embodiment 1 obtains, and add-on is to make the total sugar content in medium be 5%, yeast extract 0.2%, peptone 0....

Embodiment 3

[0038] Example 3 Succinic acid fermentation system and its coupling with ethanol fermentation system

[0039] The production method of succinic acid comprises the following steps:

[0040] (1) Plate culture: Inoculate the succinic acid bacteria species Actinobacillus succinate into the tryptone soybean agar medium, and culture it anaerobically at 35° C. for 28 hours.

[0041] (2) shake flask seed culture: medium composition is the concentrated sugar solution that embodiment 1 obtains, and add-on is to make the total sugar content in medium be 3%, yeast extract 0.4%, peptone 0.2%, corn steep liquor 0.3%, Na 2 CO 3 0.9%, NaH 2 PO 4 0.7%, K 2 HPO 4 1.0%, Na 2 HPO 4 0.2%, NaCl0.2%, tap water preparation. Inoculate the succinic acid bacteria cultured on the plate into a serum bottle containing 200ml of the above-mentioned medium for shaking flask culture, cultivate at 35°C for 15 hours, and the shaker speed is 180rpm.

[0042] (3) Seed tank culture: the medium composition i...

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Abstract

The invention relates to an alcohol and succinic acid production method by fermenting cellulosic raw material. Cellulosic raw material is pulverized, and low-concentration alkali solution is added for pre-treatment; cellulase and xylanase are performed with enzymolysis, and an enzymolysis product is filtered and concentrated to obtain concentrating sugar liquor; in an alcohol fermenting system, the concentrating sugar liquor is used for fermenting and producing alcohol; in a succinic acid fermenting system, the concentrating sugar liquor is used for fermenting and producing succinic acid; gas produced in the alcohol fermenting process is led into a succinic acid fermenting and producing system after degerming, filtering and washing. The invention ensures that a great quantity of cellulosic waste produced in farming and forestry production can be effectively utilized, solves the problem that carbon dioxide auxiliary material is additionally added during succinic acid fermentation, lowers greenhouse gas discharge value during alcohol fermentation and cost for treating tail gas, has the advantages of wide raw material resource, low production cost, high production efficiency and the like, is favourable for energy saving, emission reduction and environment protection, and is favourable for popularizing and applying.

Description

technical field [0001] The invention relates to a production method of ethanol and succinic acid, in particular to a method for producing ethanol and succinic acid by fermentation of cellulose raw materials. Background technique [0002] Succinic acid, also known as succinic acid, is a dibasic organic acid, which is an important C 4 Platform compounds, which can be used as intermediates in the synthesis of complex organic compounds, are widely used in pharmaceuticals, food processing, synthetic plastics, rubber, protective coatings, dyes and other industries. At present, more than 250 kinds of chemical products using benzene as raw materials can be obtained through the conversion of succinic acid, so the market demand for succinic acid continues to increase. [0003] There are two main methods for producing succinic acid: chemical synthesis and microbial fermentation. Due to the use of fossil raw materials and environmental pollution, the traditional chemical synthesis met...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12P7/46C12R1/19
CPCY02E50/16Y02E50/10
Inventor 李荣杰欧阳平凯薛培俭姜岷尚海涛
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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