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Small peptide C with antibacterial and antitumor functions and applications thereof

A technology of anti-cancer drugs and anti-lung cancer drugs, which is applied in the field of biomedicine to achieve the effect of convenient artificial synthesis

Inactive Publication Date: 2010-02-17
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The vast majority of anticancer chemotherapy used in humans has not yet achieved this ideal

Method used

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  • Small peptide C with antibacterial and antitumor functions and applications thereof
  • Small peptide C with antibacterial and antitumor functions and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Peptide Design and Synthesis

[0031] 1. Extract the main international antimicrobial peptide database (http: / / aps.unmc.edu / AP / main.php; http: / / penbase.immunaqua.com / ; http: / / www.bbcm.univ.trieste.it / ; http: / / www.imtech.res.in / raghava / antibp / ) of the antibacterial peptide sequence registered in, analyze the corresponding relationship between its primary structure and activity, and design artificial short peptide sequence.

[0032] 2. Use the protein analysis database http: / / www.expasy.org / to analyze the similarity of the secondary structure of the polypeptide sequence with known activity and the designed polypeptide sequence, and correct the designed sequence in combination with other physicochemical parameter predictions.

[0033] 3. Send the peptide sequence to the biological company for synthesis or use an automatic peptide synthesizer to synthesize the complete sequence. The purity of the HPLC test should be greater than 96%, and the quality of the peptide synthe...

Embodiment 2

[0037] Using MTT assay to detect tumor suppressor activity of peptides in vitro

[0038] 2.1 Materials and reagents

[0039] 1) No. 2353 peptide (named small peptide C in the present invention), synthesized by Shanghai Sangon Bioengineering Technology Service Co., Ltd.

[0040] 2) H460 cells (human non-small cell lung cancer line), H460 taxR Cells (human lung cancer cell line H460, resistant to paclitaxel), NHFB cells (human normal fibroblast cell line), Hela cells (human cervical cancer cell line), SGC-7901 cells (human gastric cancer cell line), Cell Bank of the Chinese Academy of Sciences / Cell Resource Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

[0041] 3) RPMI1640 medium, a product of GIBCO, USA

[0042] 4) DMEM medium, the product of HyClone Company in the United States

[0043] 5) Neonatal calf serum, a product of Hangzhou Sijiqing Company

[0044] 6) Dimethyl sulfoxide (DMSO), tetramethylazoazole (MTT) and trypsin are produc...

Embodiment 3

[0056] Detection of antibacterial activity of peptides by liquid growth inhibition method

[0057] 3.1 Materials and reagents

[0058] 1) Ampicillin (Amp) is a product of Solarbio Company, the disposable filter is a product of PALL Company of the United States, and other pharmaceutical reagents are of domestic analytical grade.

[0059] 2) Select Gram-positive bacteria: Bacillus subtilis (Ehrenberg) Cohn, Staphyloccocus aureus Rosenbach, Micrococcus luteus, Bacillus thuringiensis, megaspore Bacillus megaterium; Gram-negative bacteria: Escherichia coli, Vibrio, Klebsiellapneumoniae; Fungi: Fusarium solani, Fusarium oxysporum oxysporum), melon anthracnose (Colletetrichum lagenarium), apple anthracnose (Gloeosporium album) and cotton Verticillium dahliae. Provided by the strain room of the State Key Laboratory of Microbiology, Shandong University.

[0060] 3) Blocking solution: 0.4% BSA (w / v), 0.02% glacial acetic acid, filter and sterilize after preparation.

[0061] 4) PB l...

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Abstract

The invention discloses small peptide C with antibacterial and antitumor activities. The peptide C has an amino acid sequence shown in SEQ ID NO.1, has a peptide sequence with antitumor and antibacterial activities at low concentrations and has no effect on normal growth of cells of humans at corresponding concentrations. The invention also discloses applications of the small peptide in preparingantitumor and antibacterial drugs, in preparing genetically modified organisms and in preparing additives for feed, food and cosmetics.

Description

technical field [0001] The invention relates to small peptides and applications thereof, in particular to a small peptide C with dual activities of antibacterial and anticancer and applications thereof, belonging to the field of biomedicine. Background technique [0002] Cancer is a leading cause of death worldwide. In 2004, cancer deaths amounted to 7.4 million (approximately 13% of all deaths). Among various cancers, lung cancer is one of the most common malignant tumors. In recent decades, its morbidity and mortality have increased significantly, and it has become one of the cancers with the highest mortality in my country. Chemotherapy and radiotherapy are the two main methods of treating cancer besides surgical resection. Most chemotherapy drugs currently used are also toxic to normal cells, especially to those cells with vigorous metabolism, such as bone marrow cells and mucosal cells. So the problem facing medical oncologists and pharmacologists is not just to find...

Claims

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Application Information

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IPC IPC(8): C07K7/06A61K38/08A61P35/00A61P31/04C12N15/63C12N15/89A23K1/16A23L1/305A61K8/64A23K20/147A23L33/18
Inventor 康翠洁孟令军王金星
Owner SHANDONG UNIV
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