Method for preparing rapeseed peptides by mixed fermentation

A technology of mixed bacteria fermentation and rapeseed, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem that the soluble nitrogen content and biological activity need to be further improved, it is difficult to form large-scale industrial production, and the protease activity is affected. and other problems, to achieve the effect of being conducive to environmental protection, low price, and increasing the content of soluble nitrogen

Inactive Publication Date: 2010-02-24
NANJING UNIV OF FINANCE & ECONOMICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method uses rapeseed protein isolate as raw material, and the amount of enzyme preparation used in production is large and expensive, resulting in high production costs, and because the anti-nutritional factors in rapeseed meal or rapeseed protein isolate cannot be completely removed, the activity of protease is affected. , the degree of hydrolysis is not high, it is difficult to form a large-scale industrial production
[0004] CN200810243461.6 and CN200810106919.3 disclose methods for modifying rapeseed meal by biological fermentation methods. Although harmful substances such as glucosinolate in rapeseed protein are removed, rapeseed peptide or products containing rapeseed peptide are obtained, but The soluble nitrogen content and biological activity of the product still need to be further improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Preparation of liquid fermentation medium

[0019] Nutrient solution composition: glucose 3g, KH 2 PO 4 2g, 1000mL, pH 6.5. Crush the ordinary rapeseed meal to a particle size of about 100 μm, mix the rapeseed meal with nutrient solution at a solid-to-liquid ratio of 1:15 g / mL, and sterilize at 121°C for 30 minutes to obtain a liquid fermentation medium for fermentation inside the tank.

[0020] (2) Liquid fermentation

[0021] ① Preparation of Bacillus subtilis seed solution:

[0022] Incline medium: beef extract 3g, peptone 10g, NaCl 5g, agar 20g, tap water 1000mL, pH7.2-7.4.

[0023] Seed medium: beef extract 3g, peptone 10, NaCl 5g, tap water 1000mL, pH 7.2-7.4.

[0024] Preparation of fermented seeds: Pick two rings of Bacillus subtilis from the activated strain culture slant and inoculate in the seed medium, seal with 8 layers of gauze, culture on a shaking table at 35°C, 120r / min for 24 hours, and the bacteria in the fermentation broth body concentrat...

Embodiment 2

[0049] (1) Preparation of liquid fermentation medium

[0050] Nutrient solution composition: glucose 3g, KH 2 PO 4 2g, 1000mL, pH 6.5. Crush the ordinary rapeseed meal to a particle size of about 100 μm, mix it evenly with the rapeseed meal with a nutrient solution at a solid-to-liquid ratio of 1:25 g / mL, and sterilize at 121°C for 30 minutes to obtain a liquid fermentation medium for fermentation inside the tank.

[0051] (2) Liquid fermentation

[0052] ① Preparation of Bacillus subtilis seed solution:

[0053] Incline medium: beef extract 3g, peptone 10g, NaCl 5, agar 20g, tap water 1000mL, pH7.2-7.4.

[0054] Seed medium: beef extract 3g, peptone 10, NaCl 5g, tap water 1000mL, pH 7.2-7.4.

[0055] Preparation of fermented seeds: Pick two rings of Bacillus subtilis from the activated strain culture slant and inoculate in the seed medium, seal with 8 layers of gauze, culture on a shaking table at 35°C, 120r / min for 24 hours, and the bacteria in the fermentation broth ...

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Abstract

The invention relates to a method for preparing rapeseed peptides by mixed fermentation, which sufficiently utilizes the functions of hydrolyzing proteins and degrading sulfuric glucoside by microorganism and effectively improves the soluble nitrogen content and the bioactivity of the product. The method for preparing the rapeseed peptides by mixed fermentation comprises the following steps:(1) mixing rapeseed meal powder with nutrient solution to prepare liquid fermentation medium; (2) inoculating bacillus subtilis, lactobacillus and candida utilis into the fermentation medium to carry out liquid fermentation; (3) carrying out solid-liquid separation on the liquid fermentation medium to obtain the crude extract of the rapeseed peptides; and (4) purifying the crude extract to obtain the rapeseed peptides. The invention has simple technique and low cost; furthermore, different microorganisms generate a plurality of metabolites and enzymes with different enzyme cutting sites, therefore, the functions of hydrolyzing proteins and degrading sulfuric glucoside of the microorganisms are fully utilized, toxic substances of rapeseed meal are effectively degraded, proteins are hydrolyzed, and the soluble nitrogen content, the bioactivity and the yield of the rapeseed peptides of the product are improved.

Description

technical field [0001] The invention relates to a method for preparing rapeseed peptide by mixed bacteria fermentation. Background technique [0002] my country is the country with the largest rapeseed production in the world, with an annual output of nearly 12 million tons. Rapeseed meal is the main by-product of rapeseed oil processing, accounting for 65% of the total rapeseed, which contains 35% to 42% protein. The amino acid composition of rapeseed protein is reasonable, the content of lysine, cystine and methionine is relatively high, which is similar to the amino acid composition model recommended by WHO / FAO, and its nutritional value is equal to or better than that of animal protein. It is an excellent vegetable protein. However, due to the existence of some anti-nutritional factors in rapeseed meal, such as glucosinolates, phytic acid, polyphenols, etc., it is difficult to remove them during processing, so rapeseed meal can only be used in the feed or fertilizer indu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P21/06C12R1/125C12R1/72C12R1/225
Inventor 袁建鞠兴荣王立峰何荣宋佳
Owner NANJING UNIV OF FINANCE & ECONOMICS
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