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Bacillus subtilis and pyrimethanil compounded bactericide and application thereof

A technology of Bacillus subtilis and compound fungicides, applied in the directions of fungicides, applications, biocides, etc., can solve the problems of excessive pesticide residues, pollute the ecological environment of orchards, and affect the quality of fruit products, achieve fast bacteriostatic speed, and improve prevention and control. Effect, control effect Stable effect

Inactive Publication Date: 2010-05-05
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to propose a method with a good control effect on pear black spot in view of the problems that a large number of chemical pesticides are used in the current production to prevent and control pear black spot, seriously pollute the orchard ecological environment, cause pesticide residues to exceed the standard, and affect fruit quality. Bacillus subtilis pyrimethanil microbial compound fungicide and its application

Method used

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  • Bacillus subtilis and pyrimethanil compounded bactericide and application thereof
  • Bacillus subtilis and pyrimethanil compounded bactericide and application thereof
  • Bacillus subtilis and pyrimethanil compounded bactericide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Inhibitory effect of Bacillus subtilis sf-628 on mycelial growth of R. pear ringworm, black spot and anthracnose fungus.

[0021] The tested fungal pathogen strains: Pear ringworm, black spot fungus, and anthracnose bacteria were provided by the Plant Protection Institute of Jiangsu Academy of Agricultural Sciences. The tested pathogenic bacteria were transplanted on PSA medium (200g potato, 20g sucrose, 20g agar and 1000ml distilled water, pH5.6~6.6), cultured in 28℃ incubator for 5d, and set aside.

[0022] Activate the biocontrol bacteria sf-628 on the NA slope (3g beef extract, 1g yeast extract, 5g peptone, 10g sucrose, 20g agar and 1000ml distilled water, pH6.8~7.0), and then transfer to 50ml NA culture medium medium, shake (150r / m) at 28°C for 48h, and set aside. Place the mycelium block of pathogenic bacteria in the center of the PSA plate, with a diameter of 7mm; place 4 filter paper sheets diagonally around the petri dish (the distance from the center of the p...

Embodiment 2

[0026] Preparation of 13% pyrimethanil · 10 billion Bacillus subtilis wettable powder.

[0027] Drugs for testing:

[0028] 40% pyrimethanil EC was produced by Jiangsu Nanjing Gesell Chemical Industry Co., Ltd.

[0029] The 60 billion Bacillus subtilis wettable powder is produced and provided by Jiangsu Suke Agrochemical Co., Ltd.

[0030] Additive: NNO (sodium methylene dinaphthalene sulfonate).

[0031] Filling accessories: light calcium carbonate

[0032] Each component is prepared according to weight percentage:

[0033] 40% pyrimethanil EC: 32.5%; 60 billion Bacillus subtilis wettable powder: 16.7%; additive NNO: 8%; light calcium carbonate: 42.8%.

[0034] Preparation process: put pyrimethanil and Bacillus subtilis in the mixer according to the above ratio, add NNO (sodium methylene dinaphthalene sulfonate), fill it with light calcium carbonate to 100%, and mix all materials in the mixer After uniformity, it is pulverized twice by a jet mill to obtain 13% pyrimethanil...

Embodiment 3

[0036] Preparation of 10% pyrimethanil · 10 billion Bacillus subtilis suspension.

[0037] The sources of 40% pyrimethanil EC and 60 billion Bacillus subtilis wettable powder are the same as in Example 2.

[0038] Auxiliary selection: sodium lignosulfonate, OP-10; CMC; organosilicone; ethylene glycol.

[0039] Solvent of choice: water.

[0040] Each component is prepared according to weight percentage:

[0041] 40% pyrimethanil EC: 25%; 60 billion Bacillus subtilis wettable powder: 16.7%; sodium lignosulfonate: 9%; OP-10: 4%; thickener CMC: 1%; defoamer organic Silicone: 0.01%; antifreeze glycol: 5%; water: appropriate amount.

[0042] Preparation process: put pyrimethanil, Bacillus subtilis, sodium lignosulfonate, OP-10, thickener CMC, defoamer organic silicone, antifreeze ethylene glycol in the reaction kettle according to the above ratio, add water Mix evenly until 100%, and then introduce the mixture into a sand mill to grind until the material fineness is 1-5 μm; afte...

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Abstract

The invention relates to a bacillus subtilis and pyrimethanil compounded bactericide. And the finished medicament of the bacillus subtilis and pyrimethanil compounded bactericide comprises active components, an assistant and a solvent or fillers, wherein the active components account for 10-85 % of the finished medicament in percentage by weight. The active components consist of 10-90 wt% of the bacillus subtilis and the pyrimethanil. When in use, the finished medicament is diluted and sprayed on fruit trees for preventing and controlling the alternaria rot of the fruit trees. The invention has the advantages that the bacillus subtilis sf-628 and the chemical agent pyrimethanil are mixed in a certain proportion for use, which can fully develop the synergistic action of microorganisms and chemical agents so as to achieve the purposes of making respective advantages complementary to each other and improving prevention and control effects; and meanwhile, the dose of the chemical agent, the medicament cost and residuals of the chemical agent on fruits can be reduced, and the ecology environment of orchards can be improved.

Description

Technical field: [0001] The invention relates to a compound fungicide and application thereof, in particular to a compound fungicide composed of bacillus subtilis and pyrimethanil and its application in preventing and treating pear black spot. Background technique: [0002] In recent years, the cultivated area of ​​pear trees in my country has increased rapidly, and the fruit output has increased rapidly. According to statistics from the Ministry of Agriculture, in 2008 my country's pear cultivation area was 954,000 hectares, with an output of 8.165 million tons, accounting for 50.3% of the world's total pear output. With the rapid development of the national economy and the continuous improvement of people's living standards, higher and higher requirements are put forward for the quality and safety of fruits. However, in recent years, due to changes in the climate environment and the invasion of foreign harmful organisms, frequent outbreaks of major diseases and insect pes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01N43/54A01N25/04A01N25/14A01P3/00
Inventor 刘邮洲陈志谊刘永锋
Owner JIANGSU ACAD OF AGRI SCI
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