Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Rhodiola rosea callus culture method

A callus and culture method technology, applied in the field of plant tissue culture, can solve the problems affecting the expansion of clinical curative effect, low content of active ingredients, complex ingredients, etc. Effect

Inactive Publication Date: 2010-07-14
烟台汇鹏生物科技有限公司
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its complex composition and low content of active ingredients, it greatly affects the clinical efficacy of the drug and the further expansion of the market

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rhodiola rosea callus culture method
  • Rhodiola rosea callus culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: a kind of rhodiola rosea callus culture method, it comprises the steps:

[0026] a. Plant selection and aseptic treatment:

[0027] a) Select wild Rhodiola rosea fresh plants with an altitude of more than 2500m in July, wash the soil with water, soak in distilled water, and place it in an ultra-clean workbench for use; b) Take out the cleaned plant body and put it in a sterile air conditioner. bottle, soaked in 75% alcohol for 15 seconds to sterilize the surface; c) soak the alcohol-sterilized plant body in 0.1% mercuric chloride solution for 5 minutes, and keep stirring gently during the soaking process to make the plant body surface fully Exposure to mercuric chloride solution; d) Rinse the plant body sterilized by soaking in mercuric chloride solution 5 times with high-temperature sterilized distilled water, rinse the mercuric chloride solution adhering to the surface of the plant body, and then rinse the plant body Blot the water with sterile filter p...

Embodiment 2

[0033] Embodiment 2: a kind of rhodiola rosea callus culture method, it comprises the steps:

[0034] a. Plant selection and aseptic treatment:

[0035]a) Select the fresh plants of wild Rhodiola rosea with an altitude of more than 2500m in August, wash the soil with water, soak in distilled water, and place it in an ultra-clean workbench for use; b) Take out the cleaned plant body and put it in a sterile air conditioner. bottle, soaked in 75% alcohol for 15 seconds to sterilize the surface; c) soak the alcohol-sterilized plant body in 0.1% mercuric chloride solution for 5 minutes, and keep stirring gently during the soaking process to make the plant body surface fully Exposure to mercuric chloride solution; d) Rinse the plant body sterilized by soaking in mercuric chloride solution 5 times with high-temperature sterilized distilled water, rinse the mercuric chloride solution adhering to the surface of the plant body, and then rinse the plant body Blot the water with sterile ...

Embodiment 3

[0041] Example 3: Wild Rhodiola rosea, callus cultured in Example 1 and Example 2 were detected. Using the HPLC chromatographic detection method, the chromatographic column is a Diamonsil ODS C-18 alkyl silica gel column, the mobile phase is 4.6mm×250mm, methanol: water is 1:20~35:100, the ultraviolet detection wavelength is 275nm, and the column temperature is 10~ The content of salidroside was detected under the conditions of 40°C, an injection volume of 1-5 μl, and a flow rate of 0.1-2.0 ml / min. The detection method comprises the following steps:

[0042] a) Preparation of salidroside standard solution: the salidroside standard product was purchased from Liaoning Provincial Institute for Drug Control (No. 110818). Accurately weigh 5.0 mg of the salidroside standard substance in a 10ml volumetric flask, dissolve in the mobile phase, and prepare a 0.5mg / L reference substance stock solution;

[0043] b) Drawing of salidroside standard curve: draw 0.25, 0.50, 1.00, 1.50, 2.00...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a rhodiola rosea callus culture method which comprises the three steps of plant corpus selection and asepsis, callus induction culture and large-scale culture, wherein the step of callus induction culture is a core technology and is carried out by adding an abiotic elicitor SNP (10-80 micrometer mole / L) and AgNO3 (10-80 micrometer mole / L) on the basis of a basic culture medium containing MS, 0-50G / L cane sugar ), 0-10g / L Agar (), 0.1-5.0mg / L alpha-naphthylacetic acid and 0.1-5.0mg / L 6-benzylaminopurine. The method has the advantages of easy operation, high effective component content, low production cost and no pollution to environment and can reach the level of industrialized production. The culture technology of the method for producing salidroside has the advantages of invariance, high content, low cost, short cycle and very strong market competition strength.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a rhodiola rosea callus culture method. Background technique [0002] Rhodiola sachalinensis A.Bor, also known as Rhodiola rosea, is a perennial herb or subshrub of the genus Rhodiola L. in the Crassulaceae family. It is an endangered and rare medicinal plant. , is also a promising environment-adaptive drug. my country has a long history of Rhodiola application. Since the 1960s, Rhodiola rosea began to receive great attention from scientists in the former Soviet Union. Modern pharmacological studies have shown that Rhodiola rosea has anti-hypoxia, anti-cold, anti-fatigue, and anti-radiation effects. Promising environment-adaptive drugs. At present, due to its harsh natural living environment, slow growth and low annual output, coupled with the increasing demand for Rhodiola rosea, the originally scarce wild resources have been threatened unprecedentedl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
Inventor 贾景明
Owner 烟台汇鹏生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com