Extrinsic analog enzyme type bio-molecular damage antagonist and preparation method thereof
A biomolecule and enzyme-mimicking technology is applied in the field of exogenous enzyme-mimicking biomolecule damage antagonists and their preparation, which can solve the problems of high preparation process and cost, expensive raw materials, etc. simple effect
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Embodiment 1
[0020] Example 1: Component A is composed of 0.03% α-N-amino-D-glucose, 0.02% chitinase, 2.5% acetic acid by volume, and the rest is water. Component B is composed of 0.015% succinic acid and 0.015% o-hydroxybenzoic acid; component C is composed of 0.03% copper sulfate and 0.001% zinc sulfate.
Embodiment 2
[0021] Example 2: Component A is composed of 0.02% chitosan, 0.01% chitosanase, 0.2% acetic acid by volume and the rest is water. Component B is composed of 0.015% citric acid and 0.002% o-hydroxybenzoic acid; component C is composed of 0.02% copper chloride and 0.001% manganese sulfate.
Embodiment 3
[0022] Example 3: Component A is composed of 0.025% chitosan acylated derivatives, 0.003% a-amylase, 0.02% formic acid by volume fraction, and the rest is water. Component B is composed of 0.025% succinic acid and 0.004% methyl salicylate; component C is composed of 0.025% copper sulfate and 0.002% zinc sulfate.
[0023] 2. Preparation method
[0024] Preparation method 1:
[0025] For the preparation of component A, add 2.5ml of glacial acetic acid to a conical flask containing 100g of distilled water, stir well, then add 0.03g of α-N-amino-D-glucose and 0.02% chitinase in sequence. Stir until dissolved, and enzymatically hydrolyze in a water bath at 50°C for 18 hours. Stand at room temperature for 5 hours; (2) add component B, that is, a mixture of 0.015g succinic acid and 0.015g o-hydroxybenzoic acid, and stir until dissolved; (3) add component C, that is, a mixture of 0.03% copper sulfate, 0.001 % zinc sulfate mixture, fully stirred for 3.5 hours, stood in a water bath ...
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