91results about How to "Wide range of potential applications" patented technology

The invention relates to an ordered mesoporous carbon material with ultra-large aperture and controllable wall thickness and a preparation method thereof, belonging to the field of advanced nano porous materials and techniques, wherein the ordered mesoporous carbon material is prepared by using ABC triblock copolymers as mesoporous material template agent. The synthesized mesoporous carbon material has the advantages that the order of mesopores is maintained, the dimension of the mesopores is ultra-large, the specific area is large, the pore volume is large, the aperture can be controlled within 10-100nm, the wall thickness of the material can be controlled through simply changing the quantity of precursors and thereby the effective and convenient method is provided for the synthesis of the ordered mesoporous carbon material with ultra-large aperture and controllable wall thickness. The prospect of application of the material and the synthesis method in various fields is wide.

The invention provides a method for extracting nano-crystalline cellulose microfiber from cotton stalk bark. The method comprises the steps of cotton stalk bark desizing, wherein cotton stalk bark is placed into a NaOH and H2O2 mixed solution to be boiled at the temperature of 65-75 DEG C and repeatedly knocked and cleaned after being fished out till pulp is removed; desized cotton stalk bark alkali treatment, wherein the desized cotton stalk bark is placed into a 3-30% NaOH solution for alkali boiling, fished out and dried; desized cotton stalk bark TEMPO oxidation, wherein the desized cotton stalk bark is oxidized in a TEMPO/NaClO2/NaBr oxidation system, NaOH is added dropwisely so as to maintain the pH value to be 10.2-10.8, ethyl alcohol is added dropwisely after a reaction is stopped, and centrifuging is performed to obtain the nano-crystalline cellulose microfiber. According to the method, the cotton stalk bark is used as raw materials creatively for the first time, preparation of the nano-crystalline cellulose microfiber is achieved, the waste cotton stalk bark is recycled, the problem of environmental pollution brought by burning cotton stalk bark is solved, and the finally-obtained nano-crystalline cellulose microfiber is controlled to be of the nanoscale size successfully.

The invention belongs to the field of biotechnologies, and relates to a method for building efficient N-glycosylation receptor protein models in Escherichia coli by the aid of skeleton proteins Fn3 (fibronectin type III domain). The human-derived proteins Fn3 with N-glycosylation recognition sites are used as receptor proteins. The method includes steps constructing Fn3-Gly recombinant protein gene expression vectors; forming recombinant genes of the N-glycosylation recognition sites carried by the human-derived proteins Fn3; cloning the genes onto the expression vectors capable of secreting the recombinant genes onto pericoel. Recombinant proteins expressed by the vectors can be used as the prokaryote research N-glycosylation receptor protein models for N-glycosylation recombinant research. The method has the advantages that the nearly 100% N-glycosylation recombinant proteins which are good in heat stability, high in expression quantity and convenient to separate and purify can be obtain, excellent protein receptors can be provided for N-glycosylation recombinant engineering, the receptor protein models can be provided for prokaryote N-glycosylation recombinant engineering, and foundation can be laid for efficiently carrying out oligosaccharide chain analysis and function research in late periods of the N-glycosylation recombinant engineering.

The invention provides a laser ablation pulsed plasma thruster. The laser ablation pulsed plasma thruster comprises a power processing unit, a capacitor unit, a working medium, a working medium supply device, a cathode, an anode and a laser generating mechanism. The power processing unit is connected with the capacitor unit through a transmission line. The positive output end and the negative output end of the capacitor unit are connected to the cathode and the anode through transmission lines correspondingly. The capacitor unit, the transmission lines, the cathode and the anode form a discharging loop, and gas between the cathode and the anode can be ionized and accelerated. A discharging channel is formed between the cathode and the anode. The working medium supply device for fixing and conveying the working medium is arranged at one end of the discharging channel, and the working medium stretches into the discharging channel from the end. Gaps exist between the working medium and the cathode as well as between the working medium and the anode. The laser generating mechanism emits laser beams into the discharging channel to ablate the working medium, the laser beams continuously ablate the working medium to generate part of ionized gas and particulate matters, the ionized gas and the particulate matters enter the discharging channel to form plasma jets moving along the discharging channel, and thrust is generated. The laser ablation pulsed plasma thruster is high in specific impulse, total impulse, working medium utilization rate and control precision.

The present invention discloses a ruddlesden-popper (R-P) homologous perovskite structure ceramic powder. The chemical formula of the powder is: Sr[n+1]Ti[n]O[3n+1], wherein n=1,2,3 to infinite. The preparation method thereof uses a sol-gel method, and has the advantages that the products have high purity and uniformity, sintering temperature is low, the reaction is easy to control, the material composition can be adjusted, the formability is good, and Sr[n+1]Ti[n]O[3n+1] series powder of uniform particles can be prepared. The ceramic powder is a functional material with a wide application range, and shows the advantages of high dielectric constant and low dielectric loss in a dielectric constant test. In particular, the dielectric loss is very small in the high frequency region, so the powder is expected to be applied in the high-frequency field.

The invention provides a photoelectrochemical biosensor as well as a preparation method and application thereof, and belongs to the technical field of detection and analysis. The photoelectrochemicalbiosensor comprises: an electrode, which comprises an electrode substrate and a covalent organic polymer film coating the electrode substrate, wherein the covalent organic polymer film is modified with palladium nanoparticles and an aptamer; and the covalent organic polymer film is prepared by carrying out a polymerization reaction on 2,6-dihydroxynaphthalene-1,5-diformaldehyde and tris(4-aminophenyl)amine. The cathode photoelectrochemical cell sensor is successfully constructed by in-situ synthesis of the covalent organic polymer film, can be used for detecting various cells such as cancer cells by changing the type of the aptamer, and has high sensitivity and strong specificity, so that the cathode photoelectrochemical cell sensor has a good practical application value.

The invention discloses a surface modification method of an oil-soluble upconversion luminescent nanometer material. The surface modification method of the oil-soluble upconversion luminescent nanometer material is characterized by comprising the following steps: uniformly dispersing the oil-soluble upconversion luminescent nanometer material in an organic solvent, then adding mPEG2k-b-PEBEP6K and stirring; then heating to remove the organic solvent and finally, centrifugally separating to obtain precipitate, drying the precipitate, namely completing the surface modification of the oil-soluble upconversion luminescent nanometer material to obtain the oil-soluble upconversion luminescent nanometer material. The surface modification method of the oil-soluble upconversion luminescent nanometer material is characterized by simply stirring and centrifugally separating to obtain a nanomaterial with excellent water solubility; meanwhile, the nanomaterial is high in dispersion in a buffering solution similar to the biosystem.

The invention discloses a method for detecting microRNA in lung cancer cells based on a non-substrate and non-labeled electrocatalytic amplification biosensor. The preparation method of the adopted biosensor comprises the following steps of: heating dicyandiamide and ferrous salt to 490-510 DEG C in an inert gas atmosphere to self-assemble to form a precursor, heating the precursor to 890-910 DEGC in the inert gas atmosphere, and performing calcining to obtain iron-containing nitrogen-rich carbon nano-tubes; and modifying the iron-containing nitrogen-rich carbon nano-tubes and gold nano-particles to a glassy carbon electrode to obtain an AuNP/ FeCN/ GCE electrode, and fixing a thiolated capture probe on the AuNP/ FeCN/ GCE electrode through a gold-sulfur bond, wherein the capture probe issingle-stranded DNA capable of hybridizing with target microRNA. In the invention, under the condition of no H2O2, a large amount of thiophen introduced by electrocatalytic reduction greatly enhanceselectrochemical signals.

The invention discloses a preparation method of a nano silica microsphere with a surface topological structure. The method comprises the following steps that under a stirring condition, firstly, a silicon source and deionized water are mixed in a certain proportion; then, a small molecule surface active substance (R-X) is added to obtain a clear and transparent mixed solution; then, a basic catalyst is added, and a reaction is performed to generate a silica sol (a); then, an aqueous solution of ammonia is added, and a reaction is performed to generate a silica sol (b); finally, the silica sol(b) is subjected to solid-liquid separation and washed to obtain the nano silica microsphere with the surface topological structure. The nano silica microsphere with the surface topological structureand the preparation method thereof have the advantages that the preparation method is simple, the conditions are mild, the particle size is controllable, the reaction batch size is large, the method is an environmental-friendly synthesis method, and the application prospect is broad.

The invention provides an electrochemical luminescent biosensor, a preparation method and application of the electrochemical luminescent biosensor to detection of a base excision repair enzyme. The electrochemical luminescent biosensor comprises a beta-CD/GO/GCE electrode and a FeMOF/AuNPs@luminol-Hairpin probe, wherein the electrode is prepared through modification of graphene oxide and cyclodextrin to a glassy carbon electrode; the probe comprises FeMOF and AuNPs@luminol modified on the FeMOF; a hairpin structure probe is also modified on the AuNPs@luminol; and a target base of the base excision repair enzyme to be detected is designed in a stem area of the hairpin structure probe. The electrochemical luminescent biosensor provided by the invention can be used for detecting the base excision repair enzyme through triple signal amplification, so that the electrochemical luminescent biosensor has extremely high sensitivity, and can be applied in the biomedical research fields of screening of base excision repair enzyme inhibitors/activators, analysis of biological samples and the like.

The invention relates to an aqueous graphene heavy-anticorrosive coating. The coating comprises two components of A and B, wherein the component A is composed of the following substances in part by mass: 10-20 parts of the aqueous resin, 50-60 parts of anti-rust filler, 5-15 parts of aqueous graphene slurry, 0.5-1 part of functional auxiliary agent and 10-15 parts of deionized water; the componentB is a modified amine curing agent. The aqueous graphene heavy anticorrosive coating is an environment-friendly product and has the advantages of high adhesion, neutral salt spray resistance of up to3000 hr, no cracking, no foaming, room-temperature curing, high crosslink density and simple construction. In addition, the invention simultaneously provides aqueous graphene slurry and a preparationmethod of the aqueous graphene heavy-anticorrosive coating.

The invention discloses a clay film and a preparation method thereof. A single piece layer clay stripping solution is obtained first by exfoliating and dispersing clay with nanometer cellulose, and then the single piece layer clay stripping solution, carboxymethyl cellulose and water are mixed and evenly stirred and dried at a constant temperature and a constant humidity to obtain the clay film. The clay film has the advantages of excellent flame retardancy and high light transmittance. When the total absolute dry fraction of the clay in the film is >= 30%, the clay film has an excellent self-extinguishing function; when the total absolute dry fraction of the clay in the film <= 90%, the thickness is 47-59 [mu]m, and the light transmittance under the wavelength of 600 nm is 80% or above.

The invention discloses a giant surfactant and a preparation method thereof. The preparation method of the giant surfactant with single-chain nanoparticles as the tail chain comprises the following steps: (1) performing a cycloaddition reaction on hydroxyl-containing linear poly (styrene-r-benzocyclobutene) to initiate intramolecular crosslinking to obtain hydroxyl-containing single-chain nanoparticles; (2) carrying out nucleophilic substitution reaction on the hydroxyl-containing single-chain nanoparticles, and carrying out azide reaction to obtain azide group-containing single-chain nanoparticles; (3) carrying out an azide-alkyne click chemical reaction on the azide group-containing single-chain nano particles and mono-alkynyl heptavinyl polyhedral oligomeric silsesquioxane to obtain polyhedral oligomeric silsesquioxane containing the single-chain nano particles; and (4) performing a sulfydryl-double bond click chemical reaction on the polyhedral oligomeric silsesquioxane containingthe single-chain nanoparticles. The giant surfactant has a novel tail topological structure, and the preparation method has the characteristics of simple operation, efficient reaction and capability of realizing large-scale preparation.

The invention discloses a screening method of a tumor marker or a tumor marker combination. The screening method comprises the following steps: i) determining the distribution condition of nucleosomes; and/or ii) screening a tumor-related marker through one or more indexes including the nucleosome distribution condition. The invention also provides a tumor screening model construction method based on the screening method, a cancer screening, risk prediction and/or diagnosis method, and a related tumor marker combination, a kit, a system, a device, a computer readable storage medium and equipment.

The invention provides a preparation method of an azopyridines salt compound. The preparation method comprises the following synthesis steps: (1) enabling 4-aminopyridine and phenol to be subjected todiazo coupling reaction in the presence of nitrite to obtain an intermediate 4-hydroxyphenylazopyridine; (2) enabling the 4-hydroxyphenylazopyridine and chlorododecane or bromododecane to be subjected to etherification reaction, so as to obtain an azopyridine derivative; (3) carrying out reflowing reaction on the azopyridine derivative and 1,3,5-tribromomethyl benzene according to a certain ratioto generate the three-arm star type azopyridines salt compound. According to the preparation method provided by the invention, the azopyridines salt compound with a rapid cis-trans isomerism effect and a fluorescent light aggregation and induction effect can be prepared and has wide potential application in the fields of adjustable optical filters, adjustable multi-mode laser protection, counterfeiting prevention, optical gratings and the like and the fields of laser manipulators, liquid crystal self-adaptive optical systems and the like.

The invention discloses a kilowatt-grade high-power movable type laser washing system, and relates to the field of surface washing. The kilowatt-grade high-power movable type laser washing system comprises a movable type body device which comprises a kilowatt-grade high-power laser, a power source assembly and a control assembly; the kilowatt-grade high-power laser is connected with a high-power laser washing head through an optical fiber; a movable type cooling water supply device communicates with the kilowatt-grade high-power laser and the high-power laser washing head; a movable type protective gas supply device communicates with the high-power laser washing head; a sucking head of a movable type dust sucking device is arranged on the high-power laser washing head in a connected mode;and the power source assembly and the control assembly are electrically connected with the high-power laser washing head, the movable type cooling water supply device, the movable type protective gassupply device, the movable type dust sucking device and the kilowatt-grade high-power laser. The kilowatt-grade high-power movable type laser washing system can rapidly and efficiently wash a thick corrosion layer, and the potential application range is wider; and the kilowatt-grade high-power movable type laser washing system has the mobility and is convenient to use.

The invention discloses a method for promoting cut-flower Chinese roses to absorb light by using ITO nano particle sol as leaf fertilizer. The method includes the steps that 1, ITO nano particles aresynthesized; 2, ITO leaf fertilizer is synthesized; 3, the method for applying the ITO leaf fertilizer is implemented. The synthesized ITO nano particle sol is used as the leaf fertilizer for the first time, in this way, the photosynthesis of the cut-flower Chinese roses is enhanced, the range of absorption wavelength in photosynthesis of plant leaves is enlarged, and potential application is broad in photosynthesis absorption of plants.

The invention discloses a high-mildew-resistance and water-resistance soybean meal protein adhesive and a preparation method thereof, and particularly relates to the technical field of adhesives. Thehigh-mildew-resistance and water-resistant soybean meal protein adhesive is prepared from the following components in parts by mass: 10-50 parts of degreased soybean meal powder, 80-120 parts of water, 1-20 parts of a cross-linking agent, 0.2-6 part of a natural mildew inhibitor and 0.5-8 part of a viscosity regulator. The preparation method of the high-mildew-resistance and water-resistance soybean meal protein adhesive comprises the following steps: S102, uniformly mixing degreased soybean meal powder, water and a cross-linking agent to prepare viscous liquid; S104, adding a natural mildew preventive into the viscous liquid obtained in the S102, and carrying out dispersion treatment by using mechanical dispersion equipment until the temperature of the viscous liquid does not exceed 50 DEG C in the dispersion process; and S106, adding a viscosity regulator into the viscous liquid obtained in S104, and controlling the temperature not to exceed 70 DEG C in the stirring process to finally prepare the adhesive. According to the technical scheme, the problems that an existing soybean meal protein adhesive is prone to mildewing and poor in viscosity are solved, and the soybean meal protein adhesive can be used for improving the adhesion performance of artificial boards.