Preparation method of composite nano-fiber support material of silk fibroin and calcium silicate

A technology of composite nanofiber and silk fibroin, which is applied in the field of preparation of silk fibroin and calcium silicate composite nanofiber scaffold materials, to achieve good air permeability and moisture permeability, promote bone tissue repair, and excellent biological activity

Active Publication Date: 2010-10-13
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

Electrospinning silk fibroin alone has been reported, but combined with the respective advantages of silk fibroin and calcium silicate, and through elect

Method used

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  • Preparation method of composite nano-fiber support material of silk fibroin and calcium silicate

Examples

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Embodiment 1

[0021] a. Preparation of silk fibroin: put silkworm cocoons into a sodium carbonate solution with a concentration of 0.5% by mass and stir, boil for 60 minutes to remove sericin on the surface of silkworm cocoons, take them out, squeeze them dry, wash them with water, and dry them at 60°C to prepare silk For silk fiber, a mixed solution of dry silk fiber and calcium chloride, ethanol and water with a molar ratio of 1:8:2 is dissolved at 75°C to form a silk fibroin solution, and ethanol and chlorine in the solution are removed by dialysis Calcium, filtered to obtain silk fibroin solution, and then freeze-dried at -80°C to obtain silk fibroin;

[0022] b. Preparation of calcium silicate nano-whiskers: mix water, cetyltrimethylammonium bromide and ethyl silicate uniformly according to weight percentage 1:0.005:0.06, then slowly add mass Calcium hydroxide aqueous solution with a percentage concentration of 6%, until the molar ratio of calcium and silicon in the solution is 1:1, af...

Embodiment 2

[0028] a. Preparation of silk fibroin: put silkworm cocoons into a sodium carbonate solution with a concentration of 0.5% by mass and stir, boil for 60 minutes to remove sericin on the surface of silkworm cocoons, take them out, squeeze them dry, wash them with water, and dry them at 60°C to prepare silk For silk fiber, a mixed solution of dry silk fiber and calcium chloride, ethanol and water with a molar ratio of 1:8:2 is dissolved at 75°C to form a silk fibroin solution, and ethanol and chlorine in the solution are removed by dialysis Calcium, filtered to obtain silk fibroin solution, and then freeze-dried at -80°C to obtain silk fibroin;

[0029] b. Preparation of calcium silicate nano-whiskers: mix water, cetyltrimethylammonium bromide and ethyl silicate uniformly according to the weight percentage 1:0.005:0.06, and then slowly add the concentration of Calcium hydroxide aqueous solution with a mass percentage of 6%, until the molar ratio of calcium and silicon in the solu...

Embodiment 3

[0036] a. Preparation of silk fibroin: put silkworm cocoons into a sodium carbonate solution with a concentration of 0.5% by mass and stir, boil for 60 minutes to remove sericin on the surface of silkworm cocoons, take them out, squeeze them dry, wash them with water, and dry them at 60°C to prepare silk For silk fiber, a mixed solution of dry silk fiber and calcium chloride, ethanol and water with a molar ratio of 1:8:2 is dissolved at 75°C to form a silk fibroin solution, and ethanol and chlorine in the solution are removed by dialysis Calcium, filtered to obtain silk fibroin solution, and then freeze-dried at -80°C to obtain silk fibroin;

[0037] b. Preparation of calcium silicate nano-whiskers: mix water, cetyltrimethylammonium bromide and ethyl silicate uniformly according to weight percentage 1:0.005:0.06, then slowly add mass Calcium hydroxide aqueous solution with a percentage concentration of 6%, until the molar ratio of calcium and silicon in the solution is 1:1, afte...

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Abstract

The invention discloses a preparation method of a composite nano-fiber support material of silk fibroin and calcium silicate. The method comprises the following steps: preparing the silk fibroin; preparing calcium silicate nanometer whiskers; mixing a solvent, the silk fibroin and the calcium silicate nanometer whiskers to obtain spinning solution; and finally carrying out electrostatic spinning and postprocessing to obtain the composite nano-fiber support material. In the invention, composite fiber with a nanometer number grade is prepared based on the electrostatic spinning method so that a nano-fiber complex has good biocompatibility of the silk fibroin and excellent bioactivity of the calcium silicate, is more applicable to the biomedical field, and is expected to become an ideal fiber material for a bone tissue engineering support; and the composite nano-fiber support prepared by the method has higher porosity, good mechanical properties, good air permeability and moisture-penetrability, great liquid absorption quantity and simple preparation method and process, and can be degraded in vivio and promote bone injury tissue repair.

Description

technical field [0001] The invention relates to a preparation method of a bone tissue repair material, in particular to a preparation method of a silk fibroin and calcium silicate composite nanofiber support material. Background technique [0002] Bone tissue engineering is to plant in vitro cultured and expanded cells on a biomaterial with good biocompatibility and a certain spatial structure, culture them in vitro to make the cells proliferate in large numbers, and then transplant them to the desired site together. The inner cells continue to proliferate, while the biological scaffold structure is gradually degraded and absorbed, resulting in the formation of new bone tissues and organs, thereby achieving the purpose of bone repair. Therefore, choosing appropriate biomaterials and preparation methods to construct tissue engineering scaffolds is the basis of bone tissue engineering research. [0003] Silk fibroin is a natural polymer fibrous protein extracted from silk. It...

Claims

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Application Information

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IPC IPC(8): A61L27/22D01F4/02D01F1/10
Inventor 朱海霖陈建勇冯新星吴斌伟
Owner ZHEJIANG SCI-TECH UNIV
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