Pathogenic bacterium liquid-based thin-layer smear detection kit and detection method thereof

Abstract

The invention relates to a pathogenic bacterium liquid-based thin-layer smear detection kit and a detection method thereof. The detection kit comprises a preconditioning liquid, a liquefier, a diluent, a differential staining liquid and consumable items. The detection method of the detection kit comprises the following steps: adding the strongly alkaline preconditioning liquid and the liquefier into a detection sample to carry out a homogeneous treatment on the sample, adding the diluter, adopting a liquid-based centrifugal settling thin-layer preparation technology to obtain multiple uniformly-distributed pathogenic bacterium liquid-based thin-layer smears, carrying out differential staining, observing and calculating according the staining reaction, the morphology and the arrangement mode of the pathogenic bacteria, thus working out a detection result of multiple pathogenic bacteria in the sample. The invention establishes a fast and sensitive detection method based on the pathogenic bacterium liquid-based thin-layer smears, the whole process can be completed within one hour, and the detection method is simple, thereby achieving high clinical popularization values.

Description

technical field [0001] The invention relates to a liquid-based thin-layer smear detection kit for pathogenic bacteria and a detection method thereof. The detection method of this kit is mainly to homogenize the detection sample with a pretreatment solution and a liquefaction agent, then dilute it with a diluent, and then make a centrifugal sedimentation sheet to obtain multiple liquid-based thin-layer smears of pathogenic bacteria, and then Differential staining can be used to quickly detect a variety of pathogenic bacteria in the sample according to the staining reaction, morphology and arrangement of the pathogenic bacteria. Background technique [0002] Respiratory infection is a relatively common disease. Among the various pathogens of lung infection, bacteria account for the first place (about 80% for adults and about 60% for children), followed by viruses, mycoplasma, etc. Medical Science Press, PP 974-981). Streptococcus pneumoniae, which is more important in upper...

AI Technical Summary

Problems solved by technology

In order to solve the current clinical problem of not being able to quickly and sensitively provide pathogen infection detection results for samples containing pathogenic bacteria, the present invention improves on the above-mentioned human exfoliated cell liquid-based thin-layer smear technology, and provides a pathogenic bacteria liquid-based Thin-layer smear detection kit and its detection method can quickly detect various pathogenic bacteria in samples