Liquid phase chip detector based on quantum dot

Abstract

The invention relates to a liquid phase chip detector based on a quantum dot, comprising a liquid phase chip, an exciting light source, a microflow channel, an optical imaging system and an image analyzing system, wherein the liquid phase chip is a liquid phase chip of a quantum dot coding microballoon; the exciting light source takes light with single wavelength as exciting light and refracts the exciting light into a detection area of the microflow channel which is the microflow channel of a wide sheath channel with width of 0.1-5mm; the optical imaging system is CCD (Charge Coupled Device)or CMOS (Complementary Metal-Oxide-Semiconductor Transistor) or a spectrum imaging system; and the image analyzing system comprises computer hardware and image analyzing software and is used for receiving and processing data from the optical imaging system. The liquid phase chip detector has the advantages of stable and strong fluorescence, low possibility of photobleaching, wideband excitation, narrow transmission spectrum, higher stability, sensitivity and resolution, remarkably improved detection speed and efficiency, simple and compact whole structure and can be used for detecting thousands of microballoons at each second.

Description

technical field [0001] The invention belongs to the technical field of molecular biology analysis and detection, and relates to a novel liquid phase chip, in particular to a newly designed liquid phase chip detector based on quantum dots. Background technique [0002] With the rapid development of analytical and diagnostic technology, the liquid phase chip developed in the mid-1990s, known as the post-gene era, is a new type of protein research platform, which combines flow cytometry technology with traditional chip technology. A new platform for protein research. The system organically integrates colored microspheres, modern immune technology, laser technology, applied fluidics, the latest high-speed digital signal processor and computer algorithms, resulting in extremely high detection specificity and sensitivity. It can be used for clinical disease diagnosis, such as detection of cytokines, allergens and autoimmune reactions, HLA typing, SNP detection, quantitative detec...

AI Technical Summary

Problems solved by technology

[0004] In actual use, we found that the Luminex liquid-phase chip detection system and similar products have the following defects: (1) The fluorescent dye used for microsphere encoding has some inherent shortcomings in optical characteristics, such as: narrow excitation band, emission spectrum Wide, unstable light, easy photobleaching, etc., which limit its high throughput, high sensitivity and precise resolution in applications; (2) The liquid chip system uses two laser beams, of which the red laser is used to excite micro The sphere is coded to classify the fluorescence, and the laser reader is used to detect and distinguish the species; the green laser is aimed at the reporter molecule's porphyrin fluorescence, and the laser reader is used to detect the reporter fluorescent molecule

This design is designed for two kinds of fluorescent dyes, so the optical system is complicated, the volume is large, the cost is high, and the detection speed is also limited; (3) due to the use of a single microsphere through the micro sheath system, The design restricts its detection speed, only dozens of microspheres can be read per second

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