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Spirulina acclimatization method and application thereof

A technology of spirulina and preparation method, applied in chemical instruments and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of lack of soil nutrients, large material and financial resources, high treatment costs, etc. Effects of soil salinization, soil fertility enhancement, and nitrogen and phosphorus content enhancement

Inactive Publication Date: 2013-02-27
北京芳能科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

) Among the existing main methods for controlling saline-alkali land, the water washing and salt discharge adopted by the physical method are likely to cause the loss of soil nutrients, and require large material and financial resources, while the fertilization or acid-base balance adopted by the chemical method is likely to cause soil compaction and Excessive governance costs
Although physical and chemical methods can temporarily change the salinity of the soil, they cannot essentially change the soil fertility of the original saline-alkali land.

Method used

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  • Spirulina acclimatization method and application thereof
  • Spirulina acclimatization method and application thereof
  • Spirulina acclimatization method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Dealing with the saline-alkali area around the breeding farm in Taiping Town, Binhai New Area, Tianjin

[0060] Step 1: Prepare FN32 medium (basic medium) for cultivating spirulina:

[0061] Sodium bicarbonate 10g / L Sodium chloride 1.5g / L

[0062] Sodium carbonate 2.5g / L Magnesium sulfate 0.1g / L

[0063] Dipotassium hydrogen phosphate 0.4g / L Calcium chloride 0.05g / L

[0064] Sodium nitrate 1.5g / L Gibberellin 0.1mg / L

[0065] Trace element 1.5ml / L

[0066] The trace element formula is:

[0067] Boric acid 2.86mg Ferric chloride 350mg

[0068] Zinc sulfate 0.12mg Sodium molybdate 20.4mg

[0069] Manganese sulfate 1.36mg Cobalt chloride 6.5mg

[0070] Copper sulfate 0.047mg add distilled water to make the volume to 1L

[0071] The culture conditions are: temperature 30°C, light 15000 lux, pH: 9.0, growth cycle of 7 days.

[0072] Step 2: Adapt spirulina according to the use of saline soil:

[0073] (1). Salinity tolerance acclimation: For moderate or severe saline-alkali soils, spirulina is ...

Embodiment 2

[0096] Dealing with the saline-alkali area around the breeding farm in Taiping Town, Binhai New Area, Tianjin

[0097] Step 1: Prepare the culture medium (basic medium) for cultivating spirulina:

[0098] Sodium bicarbonate 12g / L Sodium chloride 2.0g / L

[0099] Sodium carbonate 3.5g / L Magnesium sulfate 0.15g / L

[0100] Dipotassium hydrogen phosphate 0.6g / L Calcium chloride 0.02g / L

[0101] Sodium nitrate 3.5g / L Gibberellin 0.2mg / L

[0102] Trace element 0.5ml / L

[0103] The culture conditions are: temperature 28°C, illumination 20000 lux, pH: 9.5, growth cycle of 6 days.

[0104] Step 2: Adapt spirulina according to the use of saline soil:

[0105] (1). Salinity tolerance domestication:

[0106] Sodium bicarbonate 12g / L Magnesium sulfate 0.15g / L

[0107] Sodium carbonate 3.5g / L Calcium chloride 0.02g / L

[0108] Dipotassium hydrogen phosphate 0.6g / L Gibberellin 0.2mg / L

[0109] Sodium nitrate 3.5g / L Trace element 0.5ml / L

[0110] Sodium chloride gradually increased from 4g / L to 10g / L

[0111] Afte...

Embodiment 3

[0127] Dealing with the saline-alkali area around the breeding farm in Taiping Town, Binhai New Area, Tianjin

[0128] Step 1: Prepare the culture medium (basic medium) for cultivating spirulina:

[0129] Sodium bicarbonate 15g / L Sodium chloride 1.0g / L

[0130] Sodium carbonate 5g / L Magnesium sulfate 0.05g / L

[0131] Dipotassium hydrogen phosphate 0.3g / L Calcium chloride 0.03g / L

[0132] Sodium nitrate 1.0g / L Gibberellin 0.3mg / L

[0133] Trace element 1ml / L

[0134] The culture conditions are: temperature 29°C, light 25000 lux, pH: 9.0, growth cycle 8 days.

[0135] Step 2: Adapt spirulina according to the use of saline soil:

[0136] (1). Salinity tolerance acclimation:

[0137] Sodium bicarbonate 15g / L Magnesium sulfate 0.05g / L

[0138] Sodium carbonate 5g / L Calcium chloride 0.03g / L

[0139] Dipotassium hydrogen phosphate 0.3g / L Gibberellin 0.3mg / L

[0140] Sodium nitrate 1g / L trace elements 1ml

[0141] Sodium chloride gradually increased from 4g / L to 8g / L

[0142] After acclimation to salinit...

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Abstract

The invention discloses a spirulina acclimatization method and application thereof. The method comprises of preparing a culture medium, carrying out salt resistance acclimatization, nitrogen and phosphorus content reduction acclimatization, to-be-treated water quality adaptation acclimatization and the like on screened algae. After the step-by-step amplification culture, the algae is introduced into kaline soil, the spirulina propagate massively by using the photosynthesis and absorbs and fixs nitrogen and phosphorus elements in the atmosphere and water sources, after the propagation, spirulina culture water is discharged, the spirulina is deposited in the soil, and the spirulina contains a large amount of elements such as nitrogen, phosphorus and the like. When the primary treatment is carried out by adopting the method, the nitrogen content in the soil is increased by 21 to 23 percent, the phosphorus content in the soil is increased by 11 to 14 percent, the total salt content is reduced by 20 to 25 percent, the nutrition element loss caused by a water washing method is avoided, the hardening on the soil and the secondary pollution to water sources and the environment caused by fertilizer application are also avoided, the soil fertility is gradually enhanced through performing the treatment many times, the soil recovery can be accelerated, and the goal of improving the ecological environment is finally achieved.

Description

Technical field [0001] The invention discloses a method for improving saline-alkali soil and increasing soil fertility by using nitrogen-fixing cyanobacteria, in particular to a method for treating saline-alkali soil by using spirulina. Background technique [0002] Saline-alkali soils are widely distributed all over the world, covering more than 30 countries on six continents. Mainly distributed in India, Pakistan, Russia, South Africa and other countries, the total area is about 956 million hectares. Among them, India and Pakistan have 8.09 million hectares, and Russia has 7.5 million hectares. The area of ​​saline-alkali land in the western United States accounts for about half of the irrigation area, and the annual output of saline-alkali is reduced by more than 25%. Land salinization is an important part of land desertification, and it is a serious threat to the resolution of a series of major problems in local agricultural and animal husbandry production, the prosperity a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C09K17/00C12R1/89C09K109/00
Inventor 单东杰李涛
Owner 北京芳能科技有限公司
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