Spirulina acclimatization method and application thereof
A technology of spirulina and preparation method, applied in chemical instruments and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of lack of soil nutrients, large material and financial resources, high treatment costs, etc. Effects of soil salinization, soil fertility enhancement, and nitrogen and phosphorus content enhancement
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Embodiment 1
[0059] Dealing with the saline-alkali area around the breeding farm in Taiping Town, Binhai New Area, Tianjin
[0060] Step 1: Prepare FN32 medium (basic medium) for cultivating spirulina:
[0061] Sodium bicarbonate 10g / L Sodium chloride 1.5g / L
[0062] Sodium carbonate 2.5g / L Magnesium sulfate 0.1g / L
[0063] Dipotassium hydrogen phosphate 0.4g / L Calcium chloride 0.05g / L
[0064] Sodium nitrate 1.5g / L Gibberellin 0.1mg / L
[0065] Trace element 1.5ml / L
[0066] The trace element formula is:
[0067] Boric acid 2.86mg Ferric chloride 350mg
[0068] Zinc sulfate 0.12mg Sodium molybdate 20.4mg
[0069] Manganese sulfate 1.36mg Cobalt chloride 6.5mg
[0070] Copper sulfate 0.047mg add distilled water to make the volume to 1L
[0071] The culture conditions are: temperature 30°C, light 15000 lux, pH: 9.0, growth cycle of 7 days.
[0072] Step 2: Adapt spirulina according to the use of saline soil:
[0073] (1). Salinity tolerance acclimation: For moderate or severe saline-alkali soils, spirulina is ...
Embodiment 2
[0096] Dealing with the saline-alkali area around the breeding farm in Taiping Town, Binhai New Area, Tianjin
[0097] Step 1: Prepare the culture medium (basic medium) for cultivating spirulina:
[0098] Sodium bicarbonate 12g / L Sodium chloride 2.0g / L
[0099] Sodium carbonate 3.5g / L Magnesium sulfate 0.15g / L
[0100] Dipotassium hydrogen phosphate 0.6g / L Calcium chloride 0.02g / L
[0101] Sodium nitrate 3.5g / L Gibberellin 0.2mg / L
[0102] Trace element 0.5ml / L
[0103] The culture conditions are: temperature 28°C, illumination 20000 lux, pH: 9.5, growth cycle of 6 days.
[0104] Step 2: Adapt spirulina according to the use of saline soil:
[0105] (1). Salinity tolerance domestication:
[0106] Sodium bicarbonate 12g / L Magnesium sulfate 0.15g / L
[0107] Sodium carbonate 3.5g / L Calcium chloride 0.02g / L
[0108] Dipotassium hydrogen phosphate 0.6g / L Gibberellin 0.2mg / L
[0109] Sodium nitrate 3.5g / L Trace element 0.5ml / L
[0110] Sodium chloride gradually increased from 4g / L to 10g / L
[0111] Afte...
Embodiment 3
[0127] Dealing with the saline-alkali area around the breeding farm in Taiping Town, Binhai New Area, Tianjin
[0128] Step 1: Prepare the culture medium (basic medium) for cultivating spirulina:
[0129] Sodium bicarbonate 15g / L Sodium chloride 1.0g / L
[0130] Sodium carbonate 5g / L Magnesium sulfate 0.05g / L
[0131] Dipotassium hydrogen phosphate 0.3g / L Calcium chloride 0.03g / L
[0132] Sodium nitrate 1.0g / L Gibberellin 0.3mg / L
[0133] Trace element 1ml / L
[0134] The culture conditions are: temperature 29°C, light 25000 lux, pH: 9.0, growth cycle 8 days.
[0135] Step 2: Adapt spirulina according to the use of saline soil:
[0136] (1). Salinity tolerance acclimation:
[0137] Sodium bicarbonate 15g / L Magnesium sulfate 0.05g / L
[0138] Sodium carbonate 5g / L Calcium chloride 0.03g / L
[0139] Dipotassium hydrogen phosphate 0.3g / L Gibberellin 0.3mg / L
[0140] Sodium nitrate 1g / L trace elements 1ml
[0141] Sodium chloride gradually increased from 4g / L to 8g / L
[0142] After acclimation to salinit...
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