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Growth factor slow release microballoon and preparation method thereof

A technology for sustained-release microsphere preparations and growth factors, which is used in pharmaceutical formulations, medical preparations with non-active ingredients, and medical preparations containing active ingredients, etc. problem, to achieve the effect of good histocompatibility, prolonged physiological activity, and improved stability

Inactive Publication Date: 2011-06-15
THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At this stage, the repair mechanism of fractures and bone defects has been preliminarily discovered, but the sustained-release dosage form of nerve growth factor suitable for bone defect repair has not been reported yet.

Method used

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  • Growth factor slow release microballoon and preparation method thereof
  • Growth factor slow release microballoon and preparation method thereof
  • Growth factor slow release microballoon and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Preparation of Growth Factor Slow-release Microspheres

[0048] Weigh 5 μg of nerve growth factor drug and dissolve it in 0.5 g of phosphate buffer, and use it as the water phase after it is completely dissolved; weigh 150 mg of monomethoxypolyethylene glycol polylactic acid (the molecular weight of polyethylene glycol is 5000, poly The polymerization ratio of ethylene glycol and polylactic acid is 1:100) dissolved in 5 g of organic solvent (dichloromethane: acetone = 6: 2) as the oil phase; mix the above water phase and oil phase, and ultrasonically disperse for 1 min to prepare an emulsion. . Add the above emulsion slowly to 20g of phosphate buffer (200mg of sodium alginate in the phosphate buffer), ultrasonically disperse for 1min, and continue to stir at room temperature at low speed for 3h, and remove the organic solvent to obtain the colloidal solution of growth factor microspheres.

Embodiment 2

[0049] Example 2 Preparation of Growth Factor Slow-release Microspheres

[0050] Weigh 10 μg of nerve growth factor drug and dissolve it in 1 g of phosphate buffer, and use it as the water phase after it is completely dissolved; weigh 300 mg of monomethoxypolyethylene glycol polylactic acid (the molecular weight of polyethylene glycol is 5000, polyethylene glycol The polymerization ratio of diol and polylactic acid (1:80) was dissolved in 10 g of organic solvent (dichloromethane: acetone = 6:2) as the oil phase; the above water phase and oil phase were mixed, ultrasonically dispersed for 1 min to prepare an emulsion, and set aside. Slowly add the above emulsion to 40g of phosphate buffer (containing 400mg of sodium alginate in the phosphate buffer), ultrasonically disperse for 1min, and continue stirring at room temperature at low speed for 5h, and remove the organic solvent to obtain the colloidal solution of growth factor microspheres.

Embodiment 3

[0051] Example 3 Preparation of Growth Factor Slow-release Microspheres

[0052] Weigh 20 μg of nerve growth factor drug and dissolve it in 2 g of phosphate buffer, and use it as the water phase after it is completely dissolved; weigh 600 mg of monomethoxypolyethylene glycol polylactic acid (the molecular weight of polyethylene glycol is 5000, polyethylene glycol The polymerization ratio of diol and polylactic acid is 1:120) dissolved in 20 g of organic solvent (dichloromethane: acetone = 6: 2) as the oil phase; the above water phase and oil phase were mixed, ultrasonically dispersed for 1 min to prepare an emulsion, and set aside. Slowly add the above emulsion to 80g of phosphate buffer (containing 800mg of sodium alginate in the phosphate buffer), ultrasonically disperse for 1min, and continue to stir at room temperature at low speed for 4h, and remove the organic solvent to obtain the colloidal solution of growth factor microspheres.

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Abstract

The invention discloses a growth factor slow release microballoon which comprises a nerve growth factor, monomethoxyl poly (ethylene glycol)-poly (lactic acid) with weight-average molecular weight of 10-10.5 ten thousand, a cosolvent, an emulsifying agent and a stabilizing agent, wherein the mass ratio of the nerve growth factor to the monomethoxy polyethylene glycol-polylactic acid is (0.8-1.2):(2.4-3.6*10<4>), and the average particle diameter of the slow release microballoon is 52.9-95.5mu m. The invention also provides a growth factor slow release microballoon preparation comprising the growth factor slow release microballoon and 6.0-9.0*10<3> parts by weight of polyoxyethylene polyoxypropylene ether. The bone injury animal experiment result shows that the growth factor slow release microballoon and the preparation thereof have preferable therapeutic effects on bone injuries, and are obviously superior to the control group and the blank group (P is less than 0.05).

Description

technical field [0001] The invention relates to the field of medicines, in particular to a growth factor monomethoxypolyethylene glycol polylactic acid sustained-release microsphere and a preparation method thereof. Background technique [0002] Growth factor (growth factor, GF) is an extracellular polypeptide signaling molecule that stimulates cell growth and proliferation. By binding to specific, high-affinity cell membrane receptors, it is a multi-effect polypeptide substance that regulates cell growth and other cell functions. Growth factors exist in platelets, various adult and embryonic tissues and most cultured cells, and have certain specificity to different types of cells, such as nerve growth factor, epithelial growth factor, platelet-derived growth factor, fibroblast growth factor, etc. . Nerve growth factor (NGF), synthesized and secreted by target tissue cells innervated by effector neurons, is a nerve cell growth regulator with dual biological functions of ne...

Claims

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Application Information

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IPC IPC(8): A61P19/08A61K47/34A61K38/18A61K9/16
Inventor 刘民谭颖徽张纲
Owner THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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