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Method for detecting quality of niaolukang particles

A detection method and particle quality technology, which can be used in measurement devices, pharmaceutical formulations, urinary system diseases, etc., can solve the problems of unstable properties of stachydrine hydrochloride, large deviation of results, and poor specificity, and achieve high repeatability, Easy to operate, good reproducibility

Active Publication Date: 2012-02-15
唐秋海
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing quality inspection standard, it is stipulated that the TLC identification of motherwort, desmodium, and Polygonatum is contained in this side; The property of stachydrine hydrochloride is unstable, and there are many factors that affect the results of TLC scanning. This method has poor repeatability, large deviation of results, and poor specificity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The quality testing method of Niaolukang Granules is as follows:

[0019] 1. Identification

[0020] (1) Take 2g of this product, add 20ml of ethanol, sonicate for 60 minutes, filter, evaporate the filtrate to dryness, add 2ml of ethanol to the residue to dissolve, and use it as the test solution; take another reference substance of stachydrine hydrochloride and add methanol to make it The solution containing 1mg per 1ml was used as the reference substance solution; according to the test of thin-layer chromatography (Appendix VI B of the Chinese Pharmacopoeia 2010 Edition), 6 μl of each of the above two solutions was drawn, and they were respectively spotted on the same silica gel G thin-layer plate to Acetone-absolute ethanol-hydrochloric acid (10:6:1) as the developer, develop, take out, dry in the air, heat at 105°C for 10 minutes, take out, let cool, spray with dilute potassium bismuth iodide-1% trichloride Iron solution (2:1), in the chromatogram of the test produ...

Embodiment 2

[0029] 1. Identification is the same as in Example 1.

[0030] 2. Content determination (high performance liquid chromatography)

[0031](1) Chromatographic conditions and system suitability test: Octadecylsilane bonded silica gel is used as filler, methanol-0.4% phosphoric acid is used as mobile phase with a volume ratio of 35:45; the detection wavelength is 360m, and the number of theoretical plates is The quercetin peak calculation is not less than 2500;

[0032] All the other steps are the same as in Example 1.

Embodiment 3

[0034] 1. Identification is the same as in Example 1.

[0035] 2) Content determination (high performance liquid chromatography)

[0036] (1) Chromatographic conditions and system suitability test: Octadecylsilane bonded silica gel is used as filler, methanol-0.4% phosphoric acid is 50:60 volume ratio solution as mobile phase; the detection wavelength is 360m, and the number of theoretical plates is The quercetin peak calculation is not less than 2500;

[0037] All the other steps are the same as in Example 1.

[0038] Quality standard of Niaolukang Granules

[0039] Niaolukang Granules

[0040] NiaoLuKangKeLi

[0041] 【 Prescription 】 Motherwort 1000g Eclipta 1000g Plantain 1000g

[0042] Rush 300g Desmodium 1000g Licorice 200g

[0043] Polygonatum 1000g Chinese yam 500g

[0044] 【 Method 】The above eight flavors and yam are crushed into coarse powder, extracted twice with dilute ethanol under reflux, each time for 1.5 hours, filtered, combined filtrate, recove...

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PUM

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Abstract

The invention discloses a method for detecting quality of niaolukang particles. The formula of the niaolukang particle is as follows: preparing 1000g of leonurus, 1000g of eclipta, 1000g of plantago asiatica, 300g of junci medulla, 1000g of desmodium, 200g of liquorice, 1000g of rhizoma polygonati and 500g of rhizoma dioscoreae into granular formulation. In the method, quercetin (C15H1107) and kaempferol (C15H10O6) in desmodium are taken as a quality control index, and compared with stachydrine in the leonurus, the ingredients are stable in property, and generate small detection error. In addition, the high performance liquid chromatography is utilized for measuring content, so that the resolution rate is higher than that of other chromatography, the speed is high, and the measurement can be completed in ten minutes to dozens of minutes; the repeatability is high; a high performance liquid chromatography column can be used repeatedly; and the automatic operation is conducted, and the analysis accuracy is high. Therefore, the accuracy, the specificity and the credibility of a detection result in the quality control process of products are improved.

Description

technical field [0001] The invention relates to the technical field of medicine detection, in particular to a quality detection method for Niaolukang granules. Background technique [0002] Niaolukang Granules have the functions of clearing away heat and dampness, invigorating the spleen and kidney. It is used for the syndrome of stranguria caused by damp-heat in the lower energizer, spleen and kidney deficiency, difficulty urinating, phlegm and astringent pain; non-gonococcal urethritis sees the above-mentioned syndromes. The prescription of Niaolukang granule is: Motherwort 1000g, Eclipta 1000g, Plantain 1000g, Rush 300g, Desmodium 1000g, Licorice 200g, Polygonatum 1000g, Chinese yam 500g. Preparation method: The above eight flavors and yam are crushed into coarse powder, extracted twice with dilute ethanol under reflux, each time for 1.5 hours, filtered, combined filtrate, recovered ethanol under reduced pressure, concentrated to a relative density of 1.10-1.15 (80°C) T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90G01N30/02A61K36/8969A61P13/02
Inventor 唐秋海
Owner 唐秋海
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