A kind of preparation method of porcine lung protein peptide

A porcine lung protein peptide and pig lung technology, which is applied in the field of porcine lung protein peptide preparation, can solve the problems of waste liquid not being fully utilized, and achieve the effects of improving utilization value, improving immunity, and being easy to absorb

Active Publication Date: 2011-12-21
SHANGHAI TRANSGENIC RES CENT
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Problems solved by technology

However, the remaining waste liquid has not been fully utilized

Method used

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  • A kind of preparation method of porcine lung protein peptide
  • A kind of preparation method of porcine lung protein peptide
  • A kind of preparation method of porcine lung protein peptide

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preparation example Construction

[0039] The invention provides a preparation method of porcine lung protein peptide, which uses the waste liquid after extracting heparin sodium from pig lung as raw material, adopts modern biological technologies such as resin desalination, enzymatic hydrolysis, ultrafiltration membrane separation, spray drying, etc. to obtain porcine lung protein peptide.

[0040] The preparation method of porcine lung protein peptide of the present invention comprises the steps:

[0041] (a) Desalting: After filtering the waste liquid after extracting heparin sodium from pig lung, desalting through resin adsorption to obtain filtrate I;

[0042] (b) enzymolysis: adding protease to the filtrate I to carry out enzymolysis reaction to obtain enzymolysis solution II;

[0043] (c) ultrafiltration membrane separation: the enzymolysis solution II is separated by ultrafiltration membrane to obtain filtrate III;

[0044] (d) drying: drying the filtrate III to obtain the porcine lung protein peptide...

Embodiment 1

[0077] Preparation of porcine lung protein peptide products

[0078] Step 1: Remove the hose and clean the pig lungs

[0079] Take 550kg pig lungs, remove the hose mechanically, wash and decontaminate with clean water, and crush them at high speed.

[0080] Step 2: Salt solution

[0081] Add 500 kg of purified water to the pulverized pig lungs, add 5% (w / w) sodium chloride, and extract for 1 hour at room temperature to facilitate the extraction of heparin.

[0082] Step 3: Compound enzyme enzymatic hydrolysis

[0083] Add animal proteolytic enzyme (purchased from Guangxi Donghenghuadao Biotechnology Co., Ltd., it is a compound enzyme, composed of papain, neutral protease, and alkaline protease in a ratio of 1:2:2), and the amount added is pig 2% of the lung mass was reacted at 50° C. for 10 hours. After the enzymatic hydrolysis reaction, the solution was heated to 85° C. and kept for 30 minutes to inactivate the enzyme in the solution.

[0084] Step 4: Resin Adsorption

...

Embodiment 2

[0099] Preparation of porcine lung protein peptide products

[0100] Step 1: Resin Adsorption

[0101] The porcine lung protein liquid after extracting heparin sodium 10m 3 Filtered through 80 mesh sieve to obtain 9.2m 3 Permeate. Add 92 kg of ion exchange resin 703 (D311) to the permeate and react for 12 hours to obtain 9.0m 3 Filtrate I.

[0102] Step 2: Alkaline protease hydrolysis

[0103] Add 46 kg of alkaline protease (2709 alkaline protease, enzyme activity: 200,000 IU, purchased from Nanning Pangbo Bioengineering Co., Ltd.) to filtrate I, and enzymolyze at 55°C for 6 hours to obtain solution II.

[0104] Step 3: Ultrafiltration Membrane Separation

[0105] The obtained solution II is separated through an ultrafiltration membrane with a molecular weight of 1000 Da to obtain a filtrate III, and the filtrate III enters the next process link. Use the retentate that has not passed the ultrafiltration membrane with a molecular weight of 1000Da for other purposes; or p...

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Abstract

The invention discloses a preparation method for pork lung protein peptide, which adopts waste liquid after the heparin sodium extraction from pork lungs as raw materials and comprises the steps of desalting, enzymolysis, ultrafiltration membrane separation and drying. The invention also discloses the pork lung protein peptide obtained by the method and an application of the pork lung protein peptide, which solve the problems of biological resource waste, environment pollution and the like of the waste liquid after the heparin sodium extraction from the pork lungs, in addition, the utilization value of products is greatly improved through the continuous processing process, the prepared pork lung protein peptide is easy to absorb and has special biological activity, the animal yield can be improved when the pork lung protein peptide is applied to high-grade feedstuff, and the pork lung protein peptide can also be used for preparing compounds for improving the immunity or can be used for preparing compounds for enhancing peripheral blood lymphocytes.

Description

technical field [0001] The invention relates to the preparation of polypeptides, in particular to a method for preparing porcine lung protein peptides. Background technique [0002] Animal husbandry is an important part of agriculture, and its position in the development of my country's national economy is becoming more and more prominent. Due to the improvement of people's living standards and the influence of traditional eating habits, pig lungs, a by-product of pig slaughtering, are considered inferior and rarely cared about. [0003] At present, the number of live pigs slaughtered is large. In 2009, 640 million pigs were slaughtered in my country, an increase of 5.7% over 2008. The pig lungs were about 600,000 tons. Due to the unreasonable industrial structure, only a small amount of pig lungs were comprehensively utilized, and most of them Abandoned waste has caused a huge waste of biological resources, and at the same time caused serious environmental pollution, and th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34C07K2/00A23K1/16A23L1/305A23K10/20A23K20/147A23L33/18
Inventor 成国祥江国永于伟潘勇
Owner SHANGHAI TRANSGENIC RES CENT
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