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Method for degradation of deoxynivalenol

A technology of deoxynivalenol and Bacillus licheniformis, applied in food science, animal feed, animal feed, etc., to achieve the effect of restoring application value

Active Publication Date: 2012-12-12
QINGDAO VLAND BIOTECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few reports on the degradation of DON by microbial strains. It has been found that both anaerobic Eubacterium and Agrobacterium can partially transform DON, and to a certain extent can reduce the toxicity of DON (Fuchs E, Binder E M, Heidler D.Krska R.Structural Characterization of metabolites after themicrobial degradation of type Atrichothecenes by the bacterial strainBBSH 797. Food Additives and Contaminants, 2002, 19(4): 379-386.; ShimaJ, Takase S, Takahashi Y, Iwai Y, Fujimoto H, Yamazaki M, Ochi K. Novel detoxification of the trichothecene mycotoxin deoxynivalenol by a soilbacterium isolated by enrichment culture. Applied and Environmental Microbiology, 1997, 63(10): 3825-3830)

Method used

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  • Method for degradation of deoxynivalenol

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation of embodiment 1 bacillus licheniformis bacterial powder

[0021] 1. Incline cultivation

[0022] Insert Bacillus licheniformis strain CGMCC 1.807 into a test tube containing broth agar medium (0.3% beef extract, 1% peptone, 0.5% sodium chloride, 1.5% agar) and culture at 37°C for 24 hours.

[0023] 2. Preparation of seed solution

[0024] Transfer the Bacillus licheniformis on the slant culture medium to the shake flask, adopt broth medium (0.3% beef extract, 1% peptone, 0.5% sodium chloride), 37°C, pH7.0, 200r / min, Incubate for 24 hours.

[0025] 3. Fermentation process:

[0026] 1T seed jar:

[0027] Fermentation Medium: Broth Medium

[0028] Culture conditions: inoculum size 8%, liquid volume 60%, 37°C, 180rpm, culture for 8-10h.

[0029] 15T fermenter:

[0030] Fermentation medium: soybean meal 4%, corn flour 2%, manganese sulfate 0.05%, magnesium sulfate 0.05%.

[0031] Culture conditions: inoculum size 5%, 37°C, 150rpm, culture for 24-26h. ...

Embodiment 2

[0035] The degradation effect of embodiment 2 bacillus licheniformis bacterial powder to DON standard substance

[0036] 1. Effect of the amount of Bacillus licheniformis powder added on the degradation rate of DON standard

[0037] According to the residence time of chyme in the gastrointestinal tract of animals, the degradation rate of the DON standard was determined by the addition of Bacillus licheniformis powder in the simulated animal stomach and intestinal environment, and the optimal addition amount of the bacteria powder was determined.

[0038] 1.1 Influence of the amount of Bacillus licheniformis powder added on the degradation rate of DON in simulated gastric juice

[0039]Add 24ml of sterilized pH2.0 PBS buffer solution, 0.25ml of pepsin solution, 0.13ml of DON standard substance with a concentration of 100ppm in the conical flask, add 0.05%, 0.10%, 0.15, 0.2%, 0.3%, 0.4 % (mass ratio) Bacillus licheniformis bacteria powder, two repetitions in each group, 37 ° C,...

Embodiment 4

[0086]Embodiment 4, diatomite promotes the degradation effect of Bacillus licheniformis to DON

[0087] Get the fermented liquid described in embodiment 1 step 4, through centrifugation, washing, freeze-drying, make Bacillus licheniformis pure bacterial powder 100g;

[0088] In the simulated animal stomach and intestinal environment, the effect of diatomite on the degradation of DON by Bacillus licheniformis was determined. 4.1 Effect of diatomaceous earth on the degradation of DON by Bacillus licheniformis in simulated gastric juice

[0089] Add 240ml of sterilized pH2.0PBS buffer solution in the Erlenmeyer flask, add 2.5ml of pepsin solution, 1.3ml of DON standard substance with a concentration of 100ppm and 0.2% (mass ratio) of pure Bacillus licheniformis powder, then add 0%, 0.05%, 0.1%, 0.2%, 0.5% (mass ratio) diatomaceous earth, two repetitions in each group; without adding pure bacteria powder, only add 0%, 0.05%, 0.1%, 0.2%, 0.5 The experimental group of % (mass rati...

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Abstract

The invention relates to a method for degradation of deoxynivalenol (DON), wherein bacterial powder of bacillus licheniformis is adopted to degrade the DON in mildewy grains. With the method of the present invention, the DON in the mildewy grains can be effectively degraded, and the application values of the mildewy grains are restored. According to the present invention, with detecting the effect of the DON degrading by the bacterial powder of the bacillus licheniformis under an in vitro simulation environment of the animal gastrointestinal tract, the optimal degradation conditions for the DON by the bacterial powder of the bacillus licheniformis are explored, and the method provides a solid foundation for further development of the bacterial powder of the bacillus licheniformis into thebiological antidote, wherein the biological antidote is provided for the degradation of zeranol in grains and corns.

Description

technical field [0001] The invention belongs to the technical field of biological detoxification, and in particular relates to a method for degrading deoxynivalenol, that is, using Bacillus licheniformis powder to degrade deoxynivalenol. Background technique [0002] Deoxynivalenol (DON), belonging to the trichothecene family of compounds, is a toxic metabolite with similar chemical structure and biological activity produced by certain species of Fusarium, and its main producing bacteria are Fusarium graminearum. DON exists in cereal crops such as wheat, barley, and corn contaminated by Fusarium, and also exists in food products. Humans and animals can produce a wide range of toxic effects after eating grains contaminated by this toxin. In addition, it often contaminates crops together with other mycotoxins such as aflatoxin, and can interact with each other after entering the human body. In recent years, studies have shown that DON may be related to human esophageal cance...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/015A23K1/00A23L5/20
Inventor 马向东徐雪梅李慧芳李佩佩
Owner QINGDAO VLAND BIOTECH GRP
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