Method for preparing silicon dioxide mesoporous spheres with adjustable pore sizes and particle sizes

A technology of silica and mesoporous spheres, applied in the direction of silica, silica, etc., to achieve good biocompatibility, wide application prospects, good drug loading and release performance

Inactive Publication Date: 2012-05-02
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the particle size of this material is 300-400 nm, the agglomeration phenomenon is serious, and the pore size is only 2.5 nm, which makes it impossible to store macromolecular drugs.
In 2008, Li Yongsheng and others disclosed a hollow mesoporous sphere with a particle size of 100 nm. The mesoporous sphere not only has a high ibuprofen storage capacity, but also the thickness of the mesoporous layer and the particle size can be fine-tuned. However, its The mesopore diameter is also very small, only 2.2nm, therefore, it also cannot solve the problem of macromolecular drug storage
At present, there are few reports on the research on silica mesoporous spheres with adjustable pore size and controllable particle size.

Method used

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  • Method for preparing silicon dioxide mesoporous spheres with adjustable pore sizes and particle sizes
  • Method for preparing silicon dioxide mesoporous spheres with adjustable pore sizes and particle sizes
  • Method for preparing silicon dioxide mesoporous spheres with adjustable pore sizes and particle sizes

Examples

Experimental program
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Effect test

Embodiment 1

[0044] See attached figure 1 . A method for preparing silica mesoporous spheres with adjustable pore size, the steps comprising:

[0045] (1) First mix deionized water, ammonia water and absolute ethanol according to the molar ratio of 1:0.15:0.7;

[0046] (2) long-chain organosilane (C 18 TMS) was mixed with tetraethyl orthosilicate (TEOS) at a molar ratio of 1:4.5;

[0047] (3) Add the mixture of ethyl orthosilicate and long-chain organosilane in step (2) to the mixed solution in step (1) dropwise according to the molar ratio of water to silicon: 1: 0.0041, and stir at room temperature for 4 hours;

[0048] (4) centrifuging the product obtained in step (3), and washing the obtained white product 3 times with ethanol;

[0049] (5) Dry the white product after washing with ethanol in step (4) at 80°C for 12 hours ；

[0050] (6) Finally, calcining the white product at a high temperature of 550° C. for 6 hours to obtain the aforementioned silica mesoporous spheres with adjust...

Embodiment 2

[0053] Implementation step is basically the same as embodiment 1, the difference is:

[0054] (1) Reduce the amount of ethanol so that the molar ratio of deionized water to absolute ethanol is 1:3.9;

[0055] (2) Mix long-chain organosilane and ethyl orthosilicate at a molar ratio of 1:5.0;

[0056] (5) The white product washed with ethanol in step (4) was dried at 100° C. for 8 hours.

[0057] For the transmission electron microscope photo of the silica mesoporous spheres prepared in Example 2, see image 3 .

[0058] The comparison results of the specific surface area, pore volume and pore diameter of the silica mesoporous spheres prepared in Examples 1 and 2 are shown in Table 1.

[0059] Table 1. Comparison results of specific surface area, pore volume and pore diameter of silica mesoporous spheres

[0060] .

Embodiment 3

[0062] Implementation content is basically the same as embodiment 2, the difference is:

[0063] Reduce the amount of silicon source added to make the molar ratio of water to silicon 1:0.00125.

[0064] The transmission electron micrograph of the silica mesoporous sphere prepared in embodiment 3 is shown in Figure 4 .

[0065] For comparison of the nitrogen adsorption-desorption results before and after loading doxorubicin on the silica mesoporous spheres prepared in Example 3, see Figure 7 ;

[0066] The sustained release curves of the silica mesoporous spheres loaded with doxorubicin prepared in Example 3 under different acidity conditions are shown in Figure 8 .

[0067] The effect of silica mesoporous spheres prepared in Example 3 on the survival rate of mouse fibroblast L929 is shown in Figure 9 .

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Abstract

The invention relates to the technical field of nano-biological materials, in particular to a method for preparing silicon dioxide mesoporous spheres with adjustable pore sizes and particle sizes. In the invention, a sol-gel method is utilized. The method comprises the following steps of: 1, mixing deionized water, ammonia water and absolute ethanol according to a ratio; 2, mixing long-chain organosilane and ethyl orthosilicate according to a ratio; 3, dripping the mixture obtained in the step 2 into the mixture obtained in the step 1, and stirring; 4, performing centrifugal separation, and washing by using ethanol; 5, drying; and 6, burning, and thus obtaining the silicon dioxide mesoporous spheres. The invention has the advantages that: the pore sizes of the silicon dioxide mesoporous spheres in the range of between 2.0 and 4.6nm can be adjusted by adjusting the ratio of water to the absolute ethanol in the step 1; the particle sizes of the silicon dioxide mesoporous spheres in the range of between 80 and 300nm can be adjusted by changing the using amount of a silicon source in the step 2; and the silicon dioxide mesoporous spheres prepared by the method have a narrower distribution range of the pore sizes and the particle sizes, higher biocompatibility and medicine loading and releasing performance, and a wider application prospect in the field of biomedicine.

Description

technical field [0001] The invention relates to the technical field of nanobiological materials, and relates to a preparation method of silica mesoporous spheres with adjustable pore diameters, and an application of the silica mesoporous spheres in loading and releasing anticancer drugs. [0002] current technology [0003] Mesoporous materials have the characteristics of uniform pores, large pore volume and specific surface area, silanol bonds, and easily chemically modified pore surfaces, making them promising drug carrier materials for storing drug molecules and controlling drug molecule release. Due to the characteristics of non-toxic, biocompatibility, thermochemical stability and less susceptible to the immune system, the research on silica mesoporous materials as drug delivery carriers has developed rapidly in recent years. . [0004] However, a large number of biomedical experiments have shown that the particle size of drug carrier materials for intravenous injection...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B33/18
Inventor 赵文茹张洪题施剑林
Owner EAST CHINA UNIV OF SCI & TECH
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