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Fermentation-separation coupling biological hydrogen-producing method for improving hydrogen-producing fermentation efficiency

A biohydrogen production and efficiency technology, applied in separation methods, fermentation, and dispersed particle separation, can solve problems such as hydrogen production rate decline, metabolic stagnation, and harmful system microorganisms, so as to improve substrate utilization and hydrogen production capacity , the effect of flexible operation mode

Inactive Publication Date: 2012-06-13
SHENYANG JIANZHU UNIVERSITY
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Problems solved by technology

[0002] In the process of biological hydrogen production by fermentation, the optimum pH for the growth and hydrogen production of hydrogen-producing bacteria is 4.5-5.5. When hydrogen is produced, a large amount of organic acids are generated, which makes the pH of the fermentation system drop rapidly. Strong inhibition of hydrogen metabolism, resulting in decreased hydrogen production rate, metabolic stagnation, and incomplete substrate utilization
The current method generally controls the pH of the fermentation system by adding alkali. While consuming a large amount of alkali, the labor intensity and production cost are also correspondingly increased; shortening the hydraulic retention time in process control can make the fermentation products discharged in time and alleviate the inhibition of some products, but At the same time, it will cause a large loss of bacteria or sludge and the short reaction time will lead to insufficient substrate utilization.
[0003] At present, the online detection device can control the pH in real time, but the automatic control system has a delayed response, which easily leads to excessive addition of acid and alkali, which endangers the microorganisms in the system
The immobilization process can reduce sludge loss, but the use of carriers occupies the effective reaction volume and reduces the hydrogen production per unit volume

Method used

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  • Fermentation-separation coupling biological hydrogen-producing method for improving hydrogen-producing fermentation efficiency

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] 1. Prepare liquid medium for biohydrogen production and place it in container 1 for use. Liquid medium (g / L): glucose 10; tryptone 4; beef extract 2; yeast juice 1; NaC1 4; K 2 HPO 4 1.5; MgC1 6H 2 O 0.1; FeSO 4 ·7H 2 O 0.08; L-cysteine ​​0.5; vitamin solution 10 mL; trace element solution 10 mL; resazurin (0.2%) 0.5 mL. Vitamin solution (g / L): cobalamin 0.01; ascorbic acid 0.025; riboflavin 0.025; citric acid 0.02; pyridoxal 0.05; folic acid 0.01; p-aminobenzoic acid 0.01; Trace element solution (g / L): MnSO 4 ·7H 2 O 0.01; ZnSO4 7H2O 0.05; H 3 BO 3 0.01; N(CH 2 COOH) 3 4.5; CaC1 2 2H 2 O 0.01; Na 2 MoO 4 0.01; CoC1 2 ·6H 2 O 0.2; AlK(SO 4 ) 2 12H 2 O 0.01.

[0012] 2. Open the three-way ventilation valve 10, fill nitrogen gas from the sampling port 4 and sampling port 16 for 5~10 minutes, close the valve c of the ventilation valve 10, and close the sampling port 16. The liquid medium in container 1 was fed into anaerobic fermentation reactor 3 ...

Embodiment 2

[0017] 1. 2.3.4. Same as embodiment 1.

[0018] 5. liquid level controller 21 starts water inlet pump 2, pumps high-concentration liquid medium into reactor 3 from medium storage tank 20, and high-concentration liquid medium substance concentration is 10 times of liquid medium in embodiment 1, Restore the liquid level of the reactor to the initial value, that is, the volume of the reaction liquid reaches 1.2L. Under the action of the mixer 5, the high-concentration medium and the separated fermentation liquid are completely mixed for hydrogen production fermentation, and the generated gas passes through the gas outlet 6 and the gas flow rate After counting 7, it was collected and separated to obtain hydrogen.

[0019] 6. Repeat step 3.4.5.6.

[0020] In the fermentation-separation coupled biological hydrogen production method in the present invention, the specific hydrogen production rate is 2.54mol H under the feeding operation mode. 2 / mol glucose, compared with the tradit...

Embodiment 3

[0022] 1. Prepare simulated organic wastewater (similar in nature to sugar factory wastewater) and place it in container 1 for use. The preparation process is as follows, add 10~15mL of waste molasses to 1L of tap water, the chemical oxygen demand concentration of the organic wastewater is 8g / L, add 5~10 grains of chemical fertilizers, so that the chemical oxygen demand: N:P=500~1000 :5:1.

[0023] 2. Open the three-way ventilation valve 10, fill nitrogen gas from the sampling port 4 and sampling port 16 for 5~20 minutes, close the valve c of the ventilation valve 10, and close the sampling port 16. The simulated organic wastewater in container 1 was fed into anaerobic fermentation reactor 3 (stirred tank reactor) through feed pump 2, with a total volume of 3.8L and a reaction zone volume of 2.5L. The temperature of the reactor was controlled at 35±1°C. Add anaerobic fermentation hydrogen-producing bacteria to the reactor 3 to make its initial concentration reach 2g / L, close ...

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Abstract

The invention relates to a fermentation-separation coupling biological hydrogen-producing method for improving hydrogen-producing fermentation efficiency. By the biological hydrogen-producing method, the problem of low efficiency and the like in the conventional process for producing hydrogen biologically by a fermentation method is solved. The fermentation-separation coupling biological hydrogen-producing method comprises the following steps of: introducing organic wastewater into a reactor into which anaerobic hydrogen-producing bacteria are added to perform anaerobic hydrogen-producing fermentation, collecting gaseous products, and separating to obtain the hydrogen; pumping biological hydrogen-producing fermentation liquor in a dilution chamber of a bipolar membrane electrodialyzer circularly; pumping a diluted acetic acid solution in a concentration chamber of the bipolar membrane electrodialyzer circularly; pumping an electrode solution in an anode chamber and a cathode chamber, performing bipolar membrane electrodialysis, and separating organic acid from the biological hydrogen-producing fermentation liquor; and returning the separated fermentation liquor to the reactor for fermenting. By the fermentation-separation coupling biological hydrogen-producing method, the yield of hydrogen can be improved by 1.3 to 1.5 times, the pH value can be controlled to 4.8 to 5.5 without addition of alkali, the utilization rate of substrates is improved by over 50 percent to reach 100 percent, the biomass is improved by 80 percent, the specific hydrogen-producing rate can reach 2.54 molH2 / mol glucose, and by the biological hydrogen-producing method, hydrogen production can be promoted greatly.

Description

Technical field [0001] The present invention involves organic wastewater or sewage biological treatment technology, and specially involves the use of organic wastewater for fermentation method biological hydrogen-to improve the fermentation of hydrogen-producing and fermentation. Background technique [0002] In the process of fermentation method biological hydrogen, the growth of hydrogen -producing bacteria and the most suitable pH of hydrogen production is 4.5 ~ 5.5, which generates a large amount of organic acid when generating hydrogen to increase the pH of the fermentation system. At the same timeHydrogen metabolism produces strong suppression, leading to a decrease in production rate, stagnation of metabolism, and incomplete substrate use.The current method generally controls the pH of the fermentation system by investing alkali, while consuming a large amount of alkali, the labor intensity and production costs are also improved accordingly; shortening the hydraulic reside...

Claims

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Application Information

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IPC IPC(8): C12P3/00C02F1/469
Inventor 唐婧
Owner SHENYANG JIANZHU UNIVERSITY
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