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Enterococcus faecalis and applications and feed additive and leavening agent thereof

A technology for feed additives and Enterococcus faecalis, which is applied in applications, animal feed, animal feed, etc., can solve the problems of high temperature tolerance, difficult bacterial activity, and poor strain characteristics, so as to achieve strong stress resistance and reduce breeding costs. Cost, the effect of improving animal immunity

Active Publication Date: 2014-09-17
BEIJING DABEINONG TECH GRP CO LTD +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the following problems exist in probiotic strains: 1. Most probiotics are lactic acid bacteria that do not produce spores, and most of them are anaerobic bacteria, which are easily affected by external environmental factors such as temperature, oxygen, moisture, and acid-base. Usually, it is difficult to maintain the activity of bacteria; 2. It is difficult for most probiotics to tolerate the high temperature during feed pelleting during feed processing; 3. The characteristics of the bacteria themselves are not good, and their ability to produce enzymes and inhibit bacteria is poor

Method used

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  • Enterococcus faecalis and applications and feed additive and leavening agent thereof
  • Enterococcus faecalis and applications and feed additive and leavening agent thereof
  • Enterococcus faecalis and applications and feed additive and leavening agent thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Screening of Enterococcus faecalis CGMCC No.5092.

[0025] 1. Primary screening

[0026] Take 5g of samples of pig manure, cow manure and chicken manure, etc., soak them in 20ml of sterile 0.85% normal saline, oscillate to disperse the samples, make the samples fully contact with the normal saline, let them stand in a constant temperature water bath at 70°C for 3 minutes, take The supernatant was centrifuged at 5000 rpm for 10 minutes, and the supernatant was discarded. Add the centrifuged bacterial cell pellet to 10ml of artificial gastric juice, let it stand at 37°C for 2 hours, then centrifuge to collect the bacterial cell, add the bacterial cell pellet to 10ml of artificial bile salt, let it stand at 37°C for 4 hours, collect the bacterial cell, dilute and apply Spread on LB medium. After culturing in a 37°C incubator for 12-24 hours, the colonies were purified and isolated. The obtained bacteria were preserved and further re-screened.

[0027] The pre...

Embodiment 2

[0033] Molecular Identification of Example 2 Enterococcus faecalis

[0034] Use the bacterial universal primers to carry out PCR identification on the screened bacterial strains: extract the template DNA according to the operating instructions of the bacterial DNA extraction kit. Use the upstream primer 5'-AGAGTT TGA TCC TGG CTC AG-3' of the 16S rRNA conservative sequence, see SEQ ID No.2 and the downstream primer 5'-GGT TACCTT GTT ACG ACT T-3, see SEQ ID No.3 for amplification Bacterial 16s rRNA gene fragment. After sequencing, it is found that the bacterium belongs to Enterococcus faecalis, and the sequencing result of the amplified fragment is shown in SEQ ID No.1.

Embodiment 3

[0035] Example 3 Verification of Stress Resistance of Enterococcus faecalis

[0036] (1) High temperature resistance

[0037] Collect Enterococcus faecalis cultured for 12 hours by centrifugation, put them in normal saline for resuspension, take samples and spread them, and count the number of live bacteria; then heat the normal saline in a water bath at 70°C for ten minutes, cool it down rapidly, and then Plate counting was carried out, and the number of residual viable bacteria was 85.3%.

[0038] (2) Tolerance of artificial gastric juice

[0039] Take 0.5ml of Enterococcus faecalis cultured for 12h, dissolve it in 4.5ml of normal saline, and use the plate count method to count the number of viable bacteria. Then take the quantitative above-mentioned bacterial liquid and add it to 4.5ml of artificial bile salt medium, let it stand at 37°C for 2h, count the number of remaining viable bacteria by plate counting method, and the number of remaining viable bacteria is 92.2%.

...

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Abstract

The invention belongs to the biologically technical field and relates to Enterococcus faecalis and applications and a feed additive and a leavening agent thereof. The Enterococcus faecalis has a good stress resistance function and a good function of benefiting life, can be dried at high temperature under the protection of a protective agent, is also capable of tolerating pelleting temperature of 90 DEG C, has a stronger inhibitory action on escherichia coli k88 and k99 and Staphylococcus aureus, has very high capacity of producing acids and cellulase and is capable of reducing pH values and decomposing certain celluloses. The biological feed additive prepared by using the Enterococcus faecalis is capable of replacing part of antibiotics in livestock breeding and aquaculture, thereby, the use of the antibiotics is reduced, the immunity of animals is improved, the conversion rate of the feed is increased, and the culture benefits are increased. The Enterococcus faecalis also can be used for leavening bean meals, cottonseed meals, green grass and the like, and is capable of effectively preventing feeds from mildewing and increasing the use ratio of nutrients in the feeds.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a strain of Enterococcus faecalis and its application, feed additive and starter. Background technique [0002] In my country's aquaculture industry, the excessive use of antibiotics has caused drug residues and a large number of drug-resistant bacteria. There are hidden dangers, so it is imminent to find safe, residue-free alternatives to antibiotics. Biological feed additives containing probiotics are an ideal substitute for some antibiotics, and probiotic strains are an important basis for developing biological feed additives with stable quality, significant effects, and high cost performance. The key issues in the research and development of probiotics are the isolation and screening of excellent strains, and a good probiotic treatment process. At present, the following problems exist in probiotic strains: 1. Most probiotics are lactic acid bacteria that do not produce spores, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K1/16C12R1/46
Inventor 杜建涛付维来刘婷王安如王丽英王松
Owner BEIJING DABEINONG TECH GRP CO LTD
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