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Solid phase extraction method for extracting total astaxanthin from haematococcus pluvialis

A technology for Haematococcus pluvialis and Haematococcus pluvialis powder is applied in the field of solid-phase extraction of astaxanthin and solid-phase extraction of astaxanthin, which can solve the problem of large initial investment in equipment, high production technical requirements and finished products. problems such as low purity, to achieve the effect of low production cost, low cost and easy recovery

Inactive Publication Date: 2012-09-12
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of supercritical extraction technology to produce astaxanthin has the disadvantages of large initial investment in equipment, high production technology requirements and low purity of finished products, and there are still great difficulties in large-scale industrial production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Rapid separation of total astaxanthin from Haematococcus pluvialis

[0035] Follow the steps below:

[0036] 1. Solid phase extraction: In a 200 mL beaker, mix 10 g of Haematococcus pluvialis powder and 2 g of silica gel evenly, add 5 mL of methanol, 13 mL of petroleum ether, and 5 mL of dichloromethane, stir and extract for 1 h, Then the mixture is loaded into a chromatographic column with a diameter of 30 mm;

[0037] 2. Rinse: drain the chromatography column, rinse with petroleum ether and dichloromethane in sequence, and drain the effluent after it is colorless;

[0038] 3. Elution: elute with methanol, collect the red part;

[0039] 4. Concentration: Distill the eluate under reduced pressure to obtain a dark red oily liquid—astaxanthin oil.

Embodiment 2

[0040] Example 2 Preparation of high-purity astaxanthin

[0041] Follow the steps below:

[0042] 1. Solid phase extraction: In a 200 mL beaker, mix 10 g of Haematococcus pluvialis powder and 10 g of silica gel evenly, add 1 mL of methanol, 20 mL of petroleum ether, and 3 mL of dichloromethane, stir and extract for 0.5 hours, and then The mixture is loaded into a chromatography column with a diameter of 30 mm;

[0043] 2. Rinse: drain the chromatography column, rinse with petroleum ether and dichloromethane in sequence, and drain the effluent after it is colorless;

[0044] 3. Elution: elute with methanol, collect the red effluent;

[0045] 4. Concentration: Distill the red effluent under reduced pressure to obtain a dark red oily liquid—astaxanthin oil;

[0046] 5. Saponification: Mix astaxanthin oil with 40g / L potassium hydroxide-methanol solution at a volume ratio of 10:1, saponify at 15°C under nitrogen protection for about 2 hours in the dark,

[0047] 6. Centri...

Embodiment 3

[0048] Example 3 Preparation of astaxanthin crystals

[0049] Follow the steps below:

[0050] 1. Solid phase extraction: In a 200 mL beaker, mix 10 g of Haematococcus pluvialis powder and 5 g of silica gel evenly, add 3 mL of methanol, 10 mL of petroleum ether, and 1 mL of dichloromethane, stir and extract for 0.1 h, and then The mixture is loaded into a chromatography column with a diameter of 30 mm;

[0051] 2. Rinse: drain the chromatography column, rinse with petroleum ether and dichloromethane in sequence, and drain the effluent after it is colorless;

[0052] 3. Elution: elute with methanol, collect the red effluent;

[0053] 4. Concentration: Distill the red effluent under reduced pressure to obtain a dark red oily liquid—astaxanthin oil;

[0054] 5. Saponification: Mix astaxanthin oil with 40g / L potassium hydroxide-methanol solution at a volume ratio of 10:1, saponify at 15°C under nitrogen protection for about 1 hour in the dark;

[0055] 6. Centrifugation: A...

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Abstract

The invention provides a solid phase extraction method for extracting total astaxanthin from haematococcus pluvialis, comprising the following steps of: (1) solid extraction: uniformly mixing haematococcus pluvialis with silica gel at the weight ratio of 1:1-5:1, adding 1-5mL of methanol, 10-20mL of petroleum ether and 1-5mL of methylene chloride into 10g of haematococcus pluvialis, stirring and extracting for 0.1-1h, and putting the extracted mixture into a chromatography column; (2) elution: carrying out elution by the use of methanol and collecting a red eluant part; and (3) condensation: carrying out underpressure distillation on the red eluant part to obtain a dark red astaxanthin oily liquid. The solid extraction method provided by the invention has advantages of simple operation step, short time, high recovery rate, low consumption of the organic solvent, little environmental pollution and low production cost, and is easy for industrial scale-up.

Description

technical field [0001] The invention relates to a solid-phase extraction method for astaxanthin, in particular to a solid-phase extraction method for extracting astaxanthin from Haematococcus pluvialis. The invention belongs to the technical field of solid phase extraction technology of separation and enrichment chemistry. Background technique [0002] Astanxantin is a carotenoid with an o-hydroxy ketone structure, its chemical name is 3,3-dihydroxy-4,4-diketone-β, β′-carotene, and its molecular formula is C 40 h 52 o 4 , with a relative molecular weight of 596.86, pure astaxanthin is a dark reddish-brown crystal with a melting point of 224°C, insoluble in water but soluble in most organic solvents. [0003] Astaxanthin has super antioxidant and free radical scavenging ability, far more than β-carotene (about 10 times), vitamin E (about 550 times), known as "super vitamin E" and " Healthy Soft Gold". Clinical and animal experiments have shown that astaxanthin has many i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24C09B61/00
Inventor 王建刚秦宝富曹斌云廖光红郑立飞
Owner NORTHWEST A & F UNIV
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