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Anti-androgenic agent, sebum secretion blocker, hair growth stimulant, and food or beverage

An anti-androgen and sebum secretion technology, applied in the field of sebum secretion inhibitors and hair growth agents, can solve the problems of no lactoferrin, no specific disclosure details, no anti-androgen effect, etc., to achieve high efficacy and safety excellent effect

Inactive Publication Date: 2012-09-12
LOTTE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no record about lactoferrin
Patent Document 6 is a document related to the anti-hair loss effect caused by the antioxidant activity of lactoferrin, and it is about reducing the adverse effects of oxidative stress generated on the scalp surface on hair follicles, and there is nothing about the anti-androgenic effect of lactoferrin records
Patent Document 7 is a document related to the improvement of frequent urination and prostatic hyperplasia derived from the anti-inflammatory activity of lactoferrin, and it is about calming inflammation that oppresses the urinary tract to facilitate urination, and there is no description about anti-androgenic effect
Non-Patent Document 1 mentions the effect of lactoferrin on sebum, but does not specifically disclose the details
[0011] As above, although the prior art discloses acne treatment effects of lactoferrin (oral administration), seborrhea (external application), anti-hair loss (external application), frequent urination improvement effect (oral administration), or sebum regulation acne treatment based on hydrolyzed milk protein However, from the point of view of the mechanism of action of lactoferrin, there is no literature mentioning the anti-androgenic effect of lactoferrin

Method used

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  • Anti-androgenic agent, sebum secretion blocker, hair growth stimulant, and food or beverage
  • Anti-androgenic agent, sebum secretion blocker, hair growth stimulant, and food or beverage
  • Anti-androgenic agent, sebum secretion blocker, hair growth stimulant, and food or beverage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Testosterone 5α-reductase inhibition test

[0039]

[0040] 1. Add 0.5 ml of 5 mM Tris-HCl buffer (pH7.2) to 0.1 ml of 3.0 mM testosterone solution (dissolved in propylene glycol).

[0041] 2. Add 0.1ml of 6.7mM NADPH solution (dissolved in 5mM Tris-HCl buffer (pH7.2)) and 0.05ml of sample solution (dissolved in 50% ethanol), preheat at 37°C, then add enzyme solution (S-9, oriental yeast (オリエンタル yeast)) 0.1ml, keep the temperature for 1 hour.

[0042] In this test, bovine-derived lactoferrin was prepared and used as lactoferrin, and Nokogiriyashi was used as a control.

[0043] 3. Then, add 3ml of dichloromethane to stop the reaction, add 0.5ml of internal standard solution (0.1mg / ml n-hexyl p-hydroxybenzoate), shake for 10 minutes, and centrifuge at 3000rmp for 10 minutes.

[0044] 4. Separate the dichloromethane layer and dry it under reduced pressure. 5 ml of methanol was added to the dried product to dissolve it to prepare a sample solution. The content of tes...

Embodiment 2

[0053] androgen receptor binding inhibition test

[0054]

[0055] 1. Use MEM medium containing 2% DCC-treated FCS to 1.0×10 4 The cell density of cells / well / 100μl seeded androgen-dependent mouse breast cancer cell SC-3 cells in 96-well microplates, at 37°C, 5% CO 2 Cultured under -95% air.

[0056] 2. After 24 hours, replace the culture medium with the addition of the test sample and 10 -8 M in DHT's HMB medium (Ham F12+MEM medium containing 0.1% BSA), cultured for 48 hours.

[0057] In this test, bovine-derived lactoferrin was prepared and used as lactoferrin, and spironolactone was used as a positive control.

[0058] 3. Next, the absorbance at 570 nm, which is the maximum absorption point of formazan blue (ブル-ボルマザン), was measured by the MTT reduction method to evaluate cell proliferation.

[0059] In order to correct the influence of attached cells, the absorbance at 650nm was also measured, and the difference between the two absorbances was used as the amount of for...

Embodiment 3

[0068] Sebocyte culture test

[0069]

[0070] 1. In this test, hamster sebocyte culture kit KB-1000 (manufactured by Kuraboo Co., Ltd.) was used for the test.

[0071] Sebocytes from normal golden hamster auricle were seeded in 24-well plates at a density of 5.0×104 cells / well. During the test, at 1×10 -6 The concentration of M was cultured in medium containing testosterone.

[0072] 2. Cultivate for several days until confluence, and then, exchange to the medium containing the test sample. The medium was exchanged with the sample medium every other day, and the culture was continued for about 2 weeks.

[0073] In this experiment, lactoferrin from bovine was prepared and used as lactoferrin, and spironolactone was used as a control.

[0074] 3. Next, the WST-8 solution was added to each well using a sebum synthesis assay kit SE-3001 (manufactured by Kuraboo Co., Ltd.), and incubated at a constant temperature of 37° C. for 30 minutes. The supernatant was measured at a w...

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PUM

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Abstract

Disclosed are: an anti-androgenic agent having a high anti-androgenic activity, has few adverse side effects, and has excellent safety performance; and a sebum secretion blocker and a hair growth stimulant, each of which comprises the anti-androgenic agent as an active ingredient. Specifically disclosed are: an anti-androgenic agent characterized by comprising lactoferrin; a sebum secretion blocker characterized by comprising the anti-androgenic agent as an active ingredient; a hair growth stimulant characterized by comprising the anti-androgenic agent as an active ingredient; and a food or beverage containing the anti-androgenic agent that comprises lactoferrin as an active ingredient.

Description

technical field [0001] The present invention relates to an antiandrogen, and a sebum secretion inhibitor and hair growth agent containing the antiandrogen as an active ingredient. Background technique [0002] Most steroid hormones are molecular types secreted by the producing organs, which bind to receptors to play their role. In the case of male hormones collectively called androgens, for example, testosterone is produced by the pituitary gland, adrenal gland, testis, etc., and enters the target organs. After being reduced to 5α-hydrotestosterone (DHT) by testosterone 5α-reductase in the cell, it binds to the receptor and acts as an androgen. [0003] Androgen is an important hormone, and if it acts excessively, it can induce various adverse symptoms such as male pattern baldness, hirsutism, seborrhea, acne, prostatic hypertrophy, prostate tumor, and precocious puberty in boys. Therefore, it is considered to improve these adverse symptoms by inhibiting the action of exces...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A23L1/305A61P17/08A61P17/14A23L2/38A23L2/66
CPCA23G1/44A23G1/46A23G3/36A23G3/44A23G3/46A23G4/14A23G4/16A23G9/32A23G9/38A23G9/40A23L2/66A23V2002/00C12Y301/21001A23L33/17A23L33/19A61K38/40A61P13/08A61P17/08A61P17/10A61P17/14A61P35/00A61P43/00A61P5/28A23V2250/54248A23V2250/032A23V2250/708A23V2200/302A61K38/482
Inventor 儿玉悠史樋口裕明成濑敦樱井孝治
Owner LOTTE CO LTD
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