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C-glycosylflavones composition, preparation method and application thereof

A flavonoid carbon glycoside composition and a technology for the composition are applied in the field of preparation of a flavonoid carbon glycoside composition, and can solve the problems of good process controllability, low transfer rate of total flavonoids, difficulty in obtaining stable composition and the like, and achieve environmental pollution-free, The effect of easy industrial production and improved utilization

Inactive Publication Date: 2012-09-19
苏州世林医药技术发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the existing macroporous resin preparation technology of total flavonoids of Trollica chinensis, the common feature is that a single concentration of ethanol-water solvent is used to elute the target extract, so the transfer rate of total flavonoids is relatively low
On the other hand, the existing technology is not sure about the composition of the total flavonoids in the product, so it is difficult to obtain a product with stable composition and good process controllability in actual production.

Method used

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  • C-glycosylflavones composition, preparation method and application thereof
  • C-glycosylflavones composition, preparation method and application thereof
  • C-glycosylflavones composition, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Investigation of the preparation process of macroporous resin

[0020] Preparation of Trollius extract: take 10kg of Trollius medicinal material, extract twice with 15 times of water, 1 hour each time, combine the extracts, concentrate under reduced pressure to 15L, add 15L ethanol to precipitate overnight, filter, concentrate under reduced pressure, Add appropriate amount of water to make 10L (equivalent to 1.0g crude drug / mL) for later use. The total flavonoid content in the extract was measured to be 33.8%, the OGA content was 5.78%, and the orientin content was 7.34%.

[0021] Static adsorption and desorption experiments for screening resins: Take 6 kinds of macroporous resins D101, AB-8, HPD100, HPD450, HPD600 and HPD826, take 1.0g each according to the dry weight of the resin, soak in 95% ethanol overnight, wash 3 times, vacuum After drying, put it into a stoppered Erlenmeyer flask, add 20mL of nasturtium extract (0.5g crude drug / mL), and shake it in a...

Embodiment 2

[0029] Embodiment 2: Preparation of Trollus flavonoids carbon glycosides composition

[0030] Get the extract in Example 1, dilute it to 0.6g crude drug / mL; add 2.5L sample solution in the glass column that has processed HPD450 resin, water, 30% ethanol, 70%, 95% ethanol respectively 3BV was subjected to gradient elution, concentrated under reduced pressure, and dried to obtain 22.0 g of composition TC-A powder from the eluted portion of 30% ethanol, and 31.0 g of composition TC-B powder from the eluted portion of 70% ethanol.

[0031] Determination of total flavonoid content in the composition by colorimetry: Accurately weigh about 5.0 mg of rutin reference substance dried to constant weight, and dilute to 25.0 mL with methanol. Pipette 0, 0.3, 0.6, 0.9, 1.2, 1.5, 1.8, 2.1 mL of the above solution respectively, put them in a 10 mL volumetric flask, add water to make up to 5 mL, add 0.3 mL of 5% sodium nitrite, and let stand for 6 minutes; add 10 % aluminum nitrate 0.3mL, let...

experiment example 1

[0035] Experimental Example 1: Investigation of Antioxidative Effects of Trollius Flavonoids and Carbon Glycosides Composition

[0036] Diphenylpicric hydrazide (DPPH) method: Take 5 mL of prepared 0.04% DPPH ethanol solution and add 1 mL of sample solutions of various concentrations, react at room temperature in the dark for 30 minutes, and measure the absorbance A at 517 nm with a spectrophotometer i , vitamin C as a control. Calculate the scavenging rate of each sample to DPPH free radicals according to the following formula.

[0037] DPPH free radical scavenging rate (%)=[1-(A i -A j ) / A 0 ]×100%

[0038] Calculate the half clearance (IC) from the clearance curve 50 ).

[0039] Result: IC of each sample on scavenging DPPH free radical 50 Values ​​are: Vc (23.9) > orientin (30.2); vitexin (35.7); OGA (36.9) > TC-A (45.6) > 2″-O-(2′″-methylbutyl)throxine Vitexin (95.2) > Composition TC-B (121.1) > 2″-O-(3′″,4′″-dimethoxybenzoyl)vitexin (182.7); 2″-O-( 3'", 4'"-dimet...

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Abstract

The invention discloses a C-glycosylflavones composition. The composition is extracted from plants of genus Trollius, and mainly contains orientin, vitexin, or vitexin esters and orientin esters. The invention also discloses a preparation method of the C-glycosylflavones composition. The preparation method is characterized by comprising extracting Trollius Chinensis Bge., performing macroporous resin column chromatography, and performing selective gradient elution with ethanol-water mixed solvent. The macroporous resin is preferably weak polarity macroporous resin. The C-glycosylflavones composition has pharmacological effects in resisting oxidation and inflammation, and inhibiting bacterial infection; and can be applied as ingredient of drugs and functional foods.

Description

technical field [0001] The invention relates to a flavonoid carbon glycoside composition, especially extracted from nasturtium. The present invention also relates to the preparation method and application of the flavone carbon glycoside composition. Background technique [0002] Trollius (Trollius) of the Ranunculaceae family is a perennial herb. In China, the flowers are used as medicine, which has the functions of clearing away heat, anti-virus, anti-bacteria and anti-inflammation. It is also often used as a functional tea drink with health care in the folk. The functional ingredients are flavonoid carbon glycosides mainly derived from vitexin and orientin. [0003] At present, the preparation process of total flavonoids from Trollius chinensis is mainly refined by macroporous resin, combined with other auxiliary treatment techniques to further improve the purity of total flavonoids. [AB-8 macroporous adsorption resin separates the total flavonoids of Trollius chinensis....

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P39/06A61P29/00A01N43/16A01P1/00A23L1/30
Inventor 刘江云蔡金娜苑红燕刘艳丽崔京浩杨世林
Owner 苏州世林医药技术发展有限公司
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