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Application of glutaraldehyde cross-linked chitosan for inhibiting growth of burkholderia cepacia complex

A Burkholderia cepacia and glutaraldehyde cross-linking technology, applied in applications, chitin polysaccharide coatings, plant growth regulators, etc., to achieve good antibacterial persistence, simple preparation process, and prevent post-harvest diseases

Inactive Publication Date: 2012-10-17
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chitosan is a linear polymer, which is soluble in acidic medium and degrades, which limits the wide application of chitosan.

Method used

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  • Application of glutaraldehyde cross-linked chitosan for inhibiting growth of burkholderia cepacia complex
  • Application of glutaraldehyde cross-linked chitosan for inhibiting growth of burkholderia cepacia complex
  • Application of glutaraldehyde cross-linked chitosan for inhibiting growth of burkholderia cepacia complex

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 prepares glutaraldehyde cross-linked chitosan (CLCG)

[0031] Weigh 2.00g of chitosan powder with a degree of deacetylation of 85%, dissolve it in 50mL of 5% acetic acid solution, and stir it on a magnetic stirrer until it becomes a uniform mucus, then add 15mL of the freshly prepared 25% glutaraldehyde solution, and continue the magnetic Stir at room temperature on a stirrer for 24 h. After 24 hours to form a hydrogel, pour off excess glutaraldehyde solution carefully, and then gently and repeatedly rinse with sterile distilled water several times to remove uncrosslinked glutaraldehyde. Pour off the excess water, filter it dry with filter paper, and put it in a -70°C refrigerator to pre-cool. Dry the glutaraldehyde hydrogel pre-cooled at -70°C in a vacuum freeze dryer, and put it in a 4°C refrigerator for later use.

[0032] The infrared spectrometry of embodiment 2 glutaraldehyde cross-linked chitosan (CLCG)

[0033] Take 1-2mg of glutaraldehyde cross-...

Embodiment 3

[0036] The X-ray diffraction analysis of embodiment 3 glutaraldehyde cross-linked chitosan (CLCG)

[0037] The chitosan and the glutaraldehyde-crosslinked chitosan prepared in Example 1 were pulverized respectively, and tested with an X-ray diffractometer (XPert PRO Holland). Working voltage 40kV, working current 25mA, Ni filter CuK 0 Line, the measured diffraction angle 2θ=5~65°.

[0038] X-ray diffraction technology is a technology that uses X-ray diffraction and scattering effects in crystals and non-crystals to perform qualitative and quantitative analysis of phases, structure types and integrity analysis. Chitosan is a high polymer that is easy to crystallize, and it has two crystal forms, namely, crystal form I and crystal form II. image 3 It is the X-ray diffraction spectrum of chitosan (Chitosan) and glutaraldehyde cross-linked chitosan (CLCG). It can be seen from the figure that the X-ray diffraction spectrum of chitosan shows two crystalline peaks at 2θ=11° and 2...

Embodiment 4

[0039] The scanning electron microscope analysis of embodiment 4 glutaraldehyde cross-linked chitosan (CLCG)

[0040] Gently rinse the glutaraldehyde-crosslinked chitosan with sterile phosphate buffer three times, then transfer to a dry, clean centrifuge tube, immerse in 2.5% glutaraldehyde and store in the refrigerator at 4°C overnight to fix the sample. Rinse 3 times with 0.1M pH 7.0 phosphate buffer, 15 min each time. Then fix with 1% (w / v) osmic acid fixative for 1-2h. Take out the fixative and rinse with 0.1M pH 7.0 phosphate buffer 3 times, 15min each time. Then use 50%, 70%, 80%, 90%, 95%, 100% (v / v) ethanol solutions to carry out gradient dehydration to the sample, then dry with a stagnation point drier, and coat the surface of the sample with palladium metal. Finally, the electron microscope (JEOL JSM-6400) was used to scan and take pictures.

[0041] The scanning electron microscope results of glutaraldehyde cross-linked chitosan (CLCG) are as follows: Figure 4 ...

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Abstract

The invention discloses an application of glutaraldehyde cross-linked chitosan for inhibiting growth of a burkholderia cepacia complex. The glutaradehyde cross-linked chitosan is prepared by the following steps of: adding chitosan into acetic acid solution, and stirring to be uniform, so as to obtain chitosan solution; mixing the chitosan solution with glutaradehyde solution, and stirring and reacting for 12-36h, so as to obtain hydrogel; and washing and drying the hydrogel, so as to obtain the glutaraldehyde cross-linked chitosan. The glutaraldehyde cross-linked chitosan prepared by the invention has appropriate crosslinking degree, has good inhibiting effect on the burkholderia cepacia complex when being used for plant cultivation, and has good high temperature stability and anti-bacteria durability; and the glutaraldehyde cross-linked chitosan prepared by the invention is used for preparing a plant preservative film or preservative coating applied to fruit vegetable transportation or stock preservation, thus postharvest diseases can be effectively prevented, and water retention and fresh keeping effects can be realized.

Description

technical field [0001] The invention relates to the technical field of plant protection biological control, in particular to the application of glutaraldehyde-crosslinked chitosan in inhibiting the growth of Burkholderia cepacia complex. Background technique [0002] Chitosan is the product of chitin after deacetylation treatment. Chitin with a degree of deacetylation greater than 55% is usually called chitosan. Its scientific name is polyglucosamine, and its chemical name is (1,4)-2. -Amino-2-deoxy-β-D-glucose is widely found in the shells of shrimps, crabs and other animals and in the cell walls of algae and fungi. [0003] Chitosan is a natural substance with a wide antibacterial spectrum, which has different degrees of inhibitory effects on bacteria, yeasts and fungi (Li et al.2008, 2010a, b, 2011a, b; Fang et al.2010; Lou et al. 2011; Algam et al. 2011). Existing experiments have shown that the combined use of chitosan and other substances can effectively reduce the l...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P1/00A23B7/16C08J3/24C08L5/08C09D105/08
Inventor 李斌周青单长林邱慧谢关林
Owner ZHEJIANG UNIV
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