Yeast strain for generating surfactant and application thereof

A yeast strain and yeast technology, applied in the field of yeast and its application, can solve the problems of secondary pollution, unsatisfactory treatment effect, biological toxicity, etc., and achieve the effect of fast growth, not easy to mutate, and simple cultivation method

Inactive Publication Date: 2012-11-07
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently commonly used surfactants such as Tween 80 have certain toxicity to organisms in the environment, and also have certain persistence, which is easy to cause secondary pollution
At the same time, the domestic biological treatment of highly oily wastewater often

Method used

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  • Yeast strain for generating surfactant and application thereof
  • Yeast strain for generating surfactant and application thereof
  • Yeast strain for generating surfactant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Isolation and purification of embodiment 1 yeast strain

[0054] 1. Culture medium preparation

[0055] (1) Basic culture medium:

[0056] (NH 4 ) 2 SO 4 1g, K 2 HPO 4 0.8g, KH 2 PO 4 0.2g, MgSO 4 ·7H 2 O 0.2g, CaCl 2 2H 2 O 0.1g, FeSO 4 ·7H 2 O 5mg, 1mL filter-sterilized vitamin solution, 1,000mL purified water, pH 5-6, medium sterilized at 121°C for 20 minutes.

[0057] The composition of the vitamin solution is as follows: niacin 100mg, vitamin B1 100mg, biotin 5mg, p-aminobenzoic acid 50mg, vitamin B12 1mg, calcium pantothenate 50mg, vitamin B6 50mg, vitamin M 50mg, 3Na EDTA 200mg, sterilized purified water Dilute to 100mL.

[0058] (2) enrichment medium:

[0059] Add 0.2% phenanthrene solution to the basic medium, adjust the pH value to 5-6, and sterilize the medium at a constant temperature of 121° C. for 20 minutes; the composition of the phenanthrene solution is: 1 g phenanthrene, 1 L n-hexane.

[0060] (3) Separation and purification medium:...

Embodiment 2

[0084] The microbiological characteristic research of embodiment 2 yeast strains

[0085] 1. Colony morphology observation

[0086] Streak-inoculate the purified cultured yeast strains on YPD solid medium, culture at 27°C until colonies grow, and observe the colony morphology. The results are as follows:

[0087] The colony characteristics of the strain are: the diameter of the colony cultivated on the YPD solid medium for 2 days is 3-4mm, the colony is round, the surface is smooth, the edges are neat, prominent, and white.

[0088] 2. Observation of cell morphology

[0089] Inoculate the strain in YPD medium and cultivate it to the OD of the culture medium at 27°C and 150rpm 600 is 0.5, observe the cell morphology of the bacterial strain in the culture medium with a microscope, and the observation results are as follows: figure 1 As shown, the cells of yeast are spherical, and the diameter of mature cells is about 5um.

[0090] 3. 26S rDNA gene sequence determination

[...

Embodiment 3

[0095] Embodiment 3 The cultivation of Pichia guilliermondii AH2 of Pichia guilliermondii AH2

[0096] 1) Prepare the inoculation mother solution of yeast strain Pichia guilliermondii AH2

[0097] Take the fresh slant strain of yeast strain Pichia guilliermondii AH2, inoculate it in naphthalene medium, cultivate it at 27°C, 150rpm, and measure the OD of the culture medium 600 value until OD 600 When the value reaches 0.5, the inoculation mother solution of P. guilliermondii AH2 bacteria is obtained, wherein, one inoculation loop of P. guilliermondii AH2 bacteria is inoculated in every 100 ml of naphthalene medium.

[0098] 2) Cultivation in hexadecane medium

[0099] The inoculation mother liquor of bacterial strain P.guilliermondii AH2 CGMCC No.4813 is inoculated in hexadecane medium, wherein, the inoculum size is 1% (v / v), promptly the ratio of the volume of inoculation mother liquor and hexadecane medium is 1 : 100, then cultured at 27°C at 150rpm under dark conditions, ...

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Abstract

The invention discloses a strain Pichia guilliermondii AH2CGMCC No. 4813 for generating a surfactant. The yeast strain can grow with C10-C24 alkane and/or polycyclic aromatic hydrocarbon as a carbon source and can simultaneously generate the surfactant, and the yeast strain has a good degradation effect of hydrophobic organic matters including alkane and polycyclic aromatic hydrocarbons. The yeast strain has a good application prospect in petroleum produced wastewater processing.

Description

technical field [0001] The invention relates to a yeast in the field of environmental biology and its application, in particular to a yeast producing a surfactant and its application in oil production wastewater treatment, which belongs to the field of yeast strains. Background technique [0002] Oily wastewater mainly comes from petroleum, petrochemical, steel, coking, gas generation stations, mechanical processing and other industrial sectors. The oily substances contained in it include natural petroleum, petroleum products, tar and its fractions, and edible animal and vegetable oils and fats class etc. If the oily wastewater produced in the production process of these industrial sectors is not recycled, it will cause a great waste of resources; if it is directly discharged into rivers, lakes or bays without treatment, it will pollute the water body, and the hydrophobic organic matter in the oily wastewater will be in the environment. An oil film is formed on the surface ...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P1/02A62D3/02C02F3/34C12R1/84C02F101/30A62D101/20
Inventor 杨敏邓艳芹张昱黑山姆吕文洲
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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