Porotic bone scaffold and preparation method thereof

A bone scaffold and porosity technology, applied in the field of biomedical tissue engineering, can solve the problems of unfavorable cell migration, difficulty in meeting the requirements of pore size, porosity, pore wall specific surface area, and low controllability of porous bone scaffolds

Active Publication Date: 2012-11-21
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
View PDF3 Cites 52 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods all have different defects and limitations
For example, the particle precipitation method has shortcomings such as irregular, too small, and uncontrollable pore communication channels, which are not conducive to cell migration; the porogen method has porogen residues that cause cytotoxicity; the scaffold prepared by the high-pressure gas foaming method is too small. Or the

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Porotic bone scaffold and preparation method thereof
  • Porotic bone scaffold and preparation method thereof
  • Porotic bone scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0046] see figure 2 , the preparation method of the porous bone scaffold of an embodiment, comprises the steps:

[0047] Step S110: dissolving the bioactive additive in the first solvent to obtain a first solution.

[0048] The bioactive additive is at least one selected from chitosan, collagen, bone morphogenic protein, icariin and epimedium.

[0049] The first solvent is selected from one of 1,4-dioxane, acetonitrile, cyclohexane, acetone, ethylene glycol, cyclohexanone and dichloromethane, preferably 1,4-dioxane. The amount of the first solvent is such that the biologically active additive is sufficiently dissolved.

[0050] Step S120: dissolving the degradable metal, the degradable inorganic substance and the degradable polymer in a second solvent to obtain a second solution.

[0051] The degradable metal is selected from one of magnesium (Mg), iron (Fe), aluminum (Al), zinc (Zn), strontium (Sr) and manganese (Mn) or magnesium (Mg), iron (Fe), aluminum An alloy formed...

Embodiment 1

[0088] 1. At room temperature, dissolve 1 part of collagen in 1,4-dioxane to obtain the first solution; weigh 25 parts of metal magnesium powder with a particle size of 300 mesh, 25 parts TCP with a particle size of 300 mesh and 100 parts of PLGA were dissolved in 1,4-dioxane to obtain a second solution. The first solution and the second solution were mixed and stirred overnight to obtain a homogeneous mixed solution.

[0089] 2. Use the MEDCAD module in the modular structure software Mimics to statistically analyze the cross-sectional image of the leg tibial defect bone, measure the size of the defect bone, and create a CAD model based on the analysis and measurement results. The CAD model is length×width×height It is a cuboid porous bone scaffold of 3×3×4cm. Export the data containing the CAD model as an STL format file, and then use the layering software that comes with the low-temperature rapid prototyping instrument to perform layering processing to determine the thickness...

Embodiment 2

[0096] 1. At room temperature, dissolve 1 part of bone morphogenic protein in acetonitrile to obtain the first solution; weigh 100 parts of metal iron powder with a particle size of 500 mesh and 100 parts of metal iron powder with a particle size of 500 mesh at a mass ratio of 1:1:5. The objective TCP and 500 parts of PCL were dissolved in acetonitrile to obtain a second solution, the first solution and the second solution were mixed, and stirred overnight to obtain a homogeneous mixed solution.

[0097] 2. Use the MEDCAD module in the modular structure software Mimics to statistically analyze the sectional image of the defective hand ulnar bone, measure the size of the defective bone, and create a CAD model based on the analysis and measurement results. The CAD model is 2 cm in diameter and 4 cm in height Export the data containing the CAD model as an STL format file, and then use the layering software that comes with low-temperature rapid prototyping for layering processing. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Apertureaaaaaaaaaa
Apertureaaaaaaaaaa
Login to view more

Abstract

The invention provides a porotic bone scaffold, which is formed by biological activity additives, degradable metal, degradable inorganic substances and degradable polymers. The porotic bone scaffold is in a shape of a cuboid, a cylinder, a cube or a cylinder with a cross section being diamond-shaped; a plurality of holes is formed in the porotic bone scaffold; the connection rate of the holes is greater than 97 percent; a plurality of micropores is formed in hole walls of the holes; a pore diameter of each hole is 100-800 mum; a pore diameter of each micropore is 5-100 mum, and a hole depth of each micropore is 1-50 mum; and the porosity of the porotic bone scaffold is 60-95 percent. Due to a porotic structure, the adhesion of bone tissue cells and the transmission of nutrients are favored, the cells can be effectively fixed so as to be differentiated and proliferated, a favorable growing environment is provided for cell growth, and the repair of defected bone tissue is favored to be realized. The invention also provides a preparation method of the porotic bone scaffold.

Description

technical field [0001] The invention relates to the technical field of biomedical tissue engineering, in particular to a porous bone support and a preparation method thereof. Background technique [0002] With the development of society, bone defects, especially long-segment bone defects, caused by population aging, trauma, bone tumors, etc., have become a thorny problem in orthopedics clinical practice. Due to the lack of suitable treatment methods, the number of disabled people is increasing year by year, which has a serious impact on social stability and people's quality of life. Traditional biological treatment methods for bone defect treatment include autologous bone graft, cancellous bone allograft, fibula and iliac crest with vascular grafts, and other bone graft methods, etc., but there are donor shortages, rejection reactions, complications at the donor site, and surgical costs. Defects such as long cycle time. Bone tissue engineering is to plant human cells into ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61F2/02
Inventor 赖毓霄张明陈淑奎秦岭
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap