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Trichoderma viride strain and application thereof

A technology for Trichoderma viride and Botrytis cinerea, which is applied in the field of biological control preparations, can solve problems such as environmental pollution, decreased control efficiency, pesticide residues, etc., and achieves the effects of increasing crop yield, strong stress resistance, and efficient control.

Active Publication Date: 2012-11-21
BEIJING LEILI MARINE BIOINDUSTRY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical control and disease-resistant varieties are currently only effective against a small number of soil-borne diseases, and have little effect on most diseases, and the use of chemical pesticides will also cause pesticide residues and cause environmental pollution; at the same time, the long-term use of chemical pesticides makes pathogenic bacteria on them Drug resistance, leading to decline in control efficacy or even failure

Method used

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  • Trichoderma viride strain and application thereof
  • Trichoderma viride strain and application thereof
  • Trichoderma viride strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1. Isolation and purification of Trichoderma viride LLM-001 (CGMCC No.5044)

[0053] Trichoderma viride (Trichoderma viride) LLM-001 (CGMCC No.5044) of the present invention is obtained from the mushrooms in Wulian County by using the method of scribing on a plate to separate and obtain, and the separation method is as follows:

[0054] ⑴Isolation of Trichoderma strains: Take seriously damaged mushroom bags, first use 75% alcohol to treat them locally for 1 minute, and then treat them with ultraviolet light on the ultra-clean workbench for 20 minutes, cut the treated parts with a sterilized blade, and inoculate A small amount of Trichoderma spores were picked up with a needle, streaked on a PDA plate, and the growth of the colony was observed regularly. Then, the Trichoderma strain was purified by the streaking method on a plate, and transferred to a PDA test tube slant for storage for later use.

[0055] (2) Screening of Trichoderma strains highly effective against cu...

Embodiment 2

[0074] 1. Trichoderma viride fermentation process

[0075] PDB medium formula: 200g potato (peeled), 20g glucose, 1000mL distilled water.

[0076] A large amount of solid fermentation medium formula (mass percentage):

[0077] Solid material: corn cob 60%, bran 40%

[0078] Inorganic salt solution: 3.5% potassium dihydrogen phosphate, 0.04% magnesium sulfate, 4% ammonium sulfate, and the rest is water.

[0079] The solid-liquid ratio is 1:1.8 (mass ratio)

[0080] Mass solid fermentation process of Trichoderma viride LLM-001 (CGMCC No.5044):

[0081] ① Strain seed liquid culture: Pick a small amount of spores of Trichoderma viride (LLM-001) CGMCC No.5044 from the slant of the test tube, transfer them to PDB liquid medium, and culture on a shaking table at 28°C for 3~5d , this is the seed solution.

[0082] ②Cultivation of solid-producing strains: Inoculate 10% of the seed solution into solid medium (500mL Erlenmeyer flask), culture at a constant temperature of 28°C for 3-...

Embodiment 3

[0085] This example provides related experiments on the control and yield increase of Trichoderma viride LLM-001 (CGMCC No. 5044) (hereinafter referred to as Trichoderma viride LLM-001) raw powder on cucumber gray mold.

[0086] 1. Drugs to be tested

[0087] Trichoderma viride LLM-001 raw powder (prepared in Example 2) 40% imimethamine wettable powder (produced by Shandong Shouguang Shuangxing Pesticide Co., Ltd., commercially available).

[0088] 2. Test crops and control objects

[0089] The test crop is cucumber, and the variety is Changchun mici;

[0090] The control object is Botrytis cinerea.

[0091] 3. The situation of the test site

[0092] The test site is located in the cucumber greenhouse planting base in Shouguang, Shandong Province. The gray mold of cucumber in the greenhouse has been serious in recent years, and it is difficult to control. The experiment was conducted in a cucumber greenhouse of 600 square meters (0.9 mu). The soil quality is light loam, and...

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Abstract

The invention provides a Trichoderma viride strain and application thereof. TheTrichoderma viride provided by the invention has a preservation number of CGMCC No.5044, and characteristics of fast growth, large sporulation capacity, wide action spectrum, strong stress resistance, and rapid colonization in large quantity in rhizosphere of plants, and can be used for the prevention and control of plant soil-borne diseases. A biological control preparation prepared from the Trichoderma viride can not only efficiently control the soil-borne plant diseases but also effectively increase the yield of crops, and has promising application prospects.

Description

technical field [0001] The invention belongs to the field of biological control of plant diseases. Specifically, the present invention relates to a Trichoderma viride capable of efficiently preventing and controlling plant soil-borne diseases and its application, and a biological control preparation prepared by using the Trichoderma viride. Background technique [0002] Soil-borne diseases of plants are an important class of plant diseases, including root rot, blight, damping-off, fusarium wilt and so on. Fungi, bacteria, nematodes, and viruses are all soil-borne pathogens that cause soil-borne diseases. They usually infect the roots of plants, causing diseases of the roots of crops and even the whole plant, causing major economic losses. [0003] For the prevention and control of plant soil-borne diseases, conventional methods include traditional chemical control, breeding of disease-resistant varieties and biological control. Chemical control and disease-resistant variet...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 汤洁
Owner BEIJING LEILI MARINE BIOINDUSTRY INC
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