Method for preparing natural red jujube skin pigment and application of natural red jujube skin pigment
A technology for red jujube peel and pigment is applied in the preparation of natural pigment and its application field, which can solve the problems of less application and achieve the effects of simple production process, good water solubility, and low requirements for production equipment.
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Embodiment 1
[0022] Weigh 500g jujube, remove jujube meat mechanically after washing with clear water, then dry and pulverize the obtained jujube skin to obtain jujube skin powder; weigh 5g jujube skin, add 125mL of 2.0g / L sodium carbonate aqueous solution, Reflux extraction at ℃ for 2 hours, the number of extractions is 2 times, combine all the filtrates, and filter to obtain the red jujube skin pigment stock solution; concentrate, dry, and crush the red jujube skin pigment stock solution to obtain powdered natural red jujube skin pigment, the extraction rate is 18.03% , the color is reddish brown.
[0023] Prepare 0.50mg / mL red jujube skin pigment solution, set aside, sterilize the prepared solid nutrient base at high temperature, pour it into a watch glass while it is hot, and cool it, then apply 3 parts each of Staphylococcus aureus and Escherichia coli, and paste on Small filter paper, drop 5 μ L red jujube peel pigment solution on the small filter paper, cultivate in the incubator fo...
Embodiment 2
[0025] Weigh 500g jujube, remove jujube meat mechanically after washing with clear water, then dry and pulverize the obtained jujube skin to obtain jujube skin powder; weigh 5g jujube skin, add 25mL of 0.8g / L sodium carbonate aqueous solution, and Reflux extraction at ℃ for 1 hour, the number of extractions is 4 times, all the filtrates are combined, and the red date skin pigment stock solution is obtained by filtration; the red date skin pigment stock solution is concentrated, dried, and crushed to obtain powdered natural red date skin pigment, and the extraction rate is 17.84% , the color is reddish brown.
[0026] Prepare 1.00mg / mL red jujube skin pigment solution, set aside, sterilize the prepared solid nutrient base at high temperature, pour it into a watch glass to cool while it is hot, then apply 3 parts each of Listeria and Proteus respectively, and paste on Small filter paper, drop 5 μ L red jujube peel pigment solution on the small filter paper, cultivate in the incu...
example 3
[0028] Weigh 500g jujube, remove jujube meat mechanically after washing with clear water, then dry and pulverize the obtained jujube skin to obtain jujube skin powder; weigh 5g jujube skin, add 75mL of 1.2g / L sodium carbonate aqueous solution, Reflux extraction at ℃ for 1.5h, the number of extractions was 3 times, all the filtrates were combined, and the red jujube skin pigment stock solution was obtained by filtration; the red jujube skin pigment stock solution was concentrated, dried, and crushed to obtain powdered natural red jujube skin pigment, with an extraction rate of 17.37 %, the color is maroon.
[0029] Prepare 0.75mg / mL red jujube skin pigment solution, set aside, sterilize the prepared solid nutrient base at high temperature, pour it into a watch glass while it is hot, and cool it, then apply 3 parts each of Lactobacillus and Shigella respectively, and paste on small filter paper Add 5 μL red jujube skin pigment solution dropwise on a small filter paper sheet, cul...
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