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Method for loading gentamicin by using TiO2 nanotube coating in situ synthesized on Ti surface

A technology of in-situ synthesis of gentamicin, applied in the direction of surface reaction electrolytic coating, coating, electrolytic coating, etc., can solve the problems of limited loading and release characteristics, antibiotics cannot be blended, etc., and achieve the purpose of inhibiting the initial adhesion of bacteria Attachment, reduce the formation of biofilm, and promote the effect of osseointegration with the surrounding

Active Publication Date: 2014-12-10
SHANGHAI JIAOTONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Loading of antibiotics onto the porous hydroxyapatite (HA) coating of Ti implants [Liu Rongfang et al., Preparation of hydroxyapatite / TiO by hydrothermal composite electrodeposition 2 Coating method, Chinese patent, CN 03104099.3], although the HA coating loaded with antibiotics can significantly prevent infection, some problems still exist, such as: antibiotics cannot be blended into the calcium phosphate coating, and the drug-loaded calcium phosphate surface Physisorption limits loading and release characteristics, etc.
There are also scholars who covalently combine vancomycin with Ti, assuming to achieve long-acting antibacterial ability, but in vivo, the role of covalently binding antibiotics to implants is questionable [Antoci, V., C.S.Adams, et al. ( 2008). The inhibition of Staphylococcus epidermidis biofilm formation by vancomycin-modified titanium alloy and implications for the treatment of periprosthetic infection. Biomaterials 29(35): 4684-4690.]

Method used

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  • Method for loading gentamicin by using TiO2 nanotube coating in situ synthesized on Ti surface
  • Method for loading gentamicin by using TiO2 nanotube coating in situ synthesized on Ti surface
  • Method for loading gentamicin by using TiO2 nanotube coating in situ synthesized on Ti surface

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Embodiment 1

[0030] Such as Figure 1-2 As described, a TiO synthesized in situ on the Ti surface 2 The method of nanotube coating loading gentamicin, first prepare TiO on the pretreated titanium substrate by anodic oxidation method 2 nanotube coating, and then the prepared TiO 2 The nanotubes were immersed in alkaline solution, and then deposited on TiO by co-precipitation 2 The nanotube coating is loaded with gentamicin, and the specific steps are as follows:

[0031] 1) TiO 2 Preparation of nanotubes: Cut the pure titanium sheet (purity > 99.9%) into 1.5cm×1.5em size, 1mm thick substrate, polish with 400 mesh, 1000 mesh, 1500 mesh metallographic sandpaper successively, and then place Ultrasonic cleaning with ethanol and acetone, and drying for later use. Connect the ground and cleaned Ti sheet to the anode, connect the Pt sheet to the cathode, mix 20ml distilled water, 180ml ethylene glycol, 0.09M NH 4 F was mixed as electrolyte, and reacted at an oxidation voltage of 20V for 30mi...

Embodiment 2

[0036] 1) TiO 2 Preparation of nanotubes: Cut pure titanium sheet (purity>99.9%) into 1.5cm×1.5cm size, 1mm thick substrate, polish with 400 mesh, 1000 mesh, 1500 mesh metallographic sandpaper successively, then place Ultrasonic cleaning with ethanol and acetone, and drying for later use. Connect the ground and cleaned Ti sheet to the anode, connect the Pt sheet to the cathode, mix 20ml of distilled water, 180ml of ethylene glycol, and 0.05M NH4F as the electrolyte, and react at an oxidation voltage of 30V for 120min. Carried out in a fume hood, the electrolytic cell was placed in a magnetic stirrer for 10r / min magnetic stirring. After the reaction, the Ti sheet was taken out and washed with distilled water.

[0037] 2) Heat treatment of the sample: in a vacuum muffle furnace, the temperature was raised from room temperature to 550°C at a rate of 5°C / min, kept for 2 hours, and then the temperature was lowered with the furnace.

[0038] 3) Loading of gentamicin: immerse the ...

Embodiment 3

[0041] 1) TiO 2 Preparation of nanotubes: Cut pure titanium sheet (purity>99.9%) into 1.5cm×1.5cm size, 1mm thick substrate, polish with 400 mesh, 1000 mesh, 1500 mesh metallographic sandpaper successively, then place Ultrasonic cleaning with ethanol and acetone, and drying for later use. Connect the ground and cleaned Ti sheet to the anode, connect the Pt sheet to the cathode, mix 60ml of distilled water, 140ml of ethylene glycol, and 0.13M NH4F as the electrolyte, and react at an oxidation voltage of 60V for 30min. Carried out in a fume hood, the electrolytic cell was placed in a magnetic stirrer for 10r / min magnetic stirring. After the reaction, the Ti sheet was taken out and washed with distilled water.

[0042] 2) Heat treatment of the sample: in a vacuum muffle furnace, the temperature was raised from room temperature to 450°C at a rate of 2°C / min, kept for 4 hours, and then cooled with the furnace.

[0043] 3) Loading of gentamicin: the prepared sample was immersed i...

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Abstract

The invention relates to a method for loading gentamicin by using a TiO2 nanotube coating in situ synthesized on the Ti surface. The method comprises the following steps of: firstly, preparing a TiO2 nanotube coating on a preprocessed Ti substrate by using an anodic oxidation method; secondly, immersing the prepared TiO2 nanotube into an alkali solution; and thirdly, loading the gentamicin in the TiO2 nanotube coating by using a coprecipitation method. Compared with the prior art, the invention provides a preparation path of the TiO2 nanotube coating, which is simple in process and favorable in biocompatibility, and a mode for medicine loading / releasing by using the TiO2 nanotube, as well as a novel method for prevention and treatment of prosthesis-related infections after artificial joint replacement.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, in particular to a method for in-situ synthesis of TiO on the surface of Ti by anodic oxidation 2 Nanotube coating, and then use the co-precipitation technique on TiO 2 Method for loading nanotubes with gentamicin. Background technique [0002] Infection is one of the most serious complications after artificial joint replacement. Due to the long-term and difficult treatment, periprosthetic infection brings great pressure to patients, clinicians and medical institutions. The fundamental reason why infection after artificial joint replacement is difficult to cure is the formation of biofilm on the surface of the prosthesis and the lack of immunity at the prosthesis / tissue interface. The combination of biofilm formation and lack of immunity at the prosthesis / tissue interface predisposes the prosthesis to bacterial colonization and further infection. In the pathogenesis of artificial ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/30A61L27/54C25D11/26
Inventor 李华刘忠堂王珮刘河洲王立强
Owner SHANGHAI JIAOTONG UNIV
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