Fast drug sensitivity detection kit for aquatic product vibrio

Abstract

The invention relates to a fast drug sensitivity detection kit for vibrio, which can be applied to an aquiculture site. According to the invention, the detection kit comprises a detection plate, a thallus collection tool, bacterium enrichment liquid, a drug sensitivity detection culture medium, a turbidimetry tube, an inoculation burette and a colorimetric card, wherein the detection plate comprises inspection holes enveloping drugs and color indicators for drug sensitivity detection, and the color indicators are Alamar Blue. According to the invention, fish peptone, Ca2+ and Mg2+ are added based on the MH culture medium and the concentration of NaCl is adjusted, and the culture medium is used as the bacterium enrichment liquid of vibrio, which can effectively promote the growth of the vibrio and enables the bacterium enrichment liquid to reach the required concentration in a short time, so as to shorten the time of drug sensitivity detection. At the same time, a blue dye, namely the Alamar Blue, which is safe and nontoxic to the cells is used; the Alamar Blue has no toxic effect on cells, and can be enveloped in the micropores of the drug sensitivity detection plate directly without influencing the growth of the bacteria, so that the detection operation is simpler and more convenient and quick; and the proliferation dynamic of the bacteria can be continuously monitored.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnosis of aquatic pathogenic microorganisms, and in particular relates to a rapid drug sensitivity detection kit for aquatic Vibrio. Background technique [0002] With the rapid development of aquaculture in recent years, the problem of aquaculture animal diseases has become increasingly prominent, among which bacterial diseases have caused huge losses to the fishery economy worldwide. Vibrio is a large group of Gram-negative bacteria with short, polar flagella, motile, non-spore-shaped, short rods bent into arcs, belonging to the family Vibrio and the genus Vibrio. Vibrio is widely distributed in nature, mostly in water bodies. A large number of studies have shown that a variety of Vibrio can cause epidemics and explosive deaths of commercial animals such as fish, shellfish, and crustaceans in seawater, mainly including Vibrio parahaemolyticus, Vibrio brilliant, Vibrio anguillarum, and Vibri...

AI Technical Summary

Problems solved by technology

[0003] At present, in the field of aquatic bacterial disease prevention and control, the drug susceptibility testing methods mainly use the K-B disk method and broth dilution method, of which the K-B disk method generally must be carried out under the conditions of a professional microbiological laboratory, and requires personnel with professional knowledge to operate Completed, there are problems such as cumbersome operation, heavy workload, long cycle, etc., and the minimum inhibitory concentration (MIC) value of the drug cannot be obtained

Although the conventional broth dilution method can determine the MIC value of the drug, it sometimes requires the assistance of professional equipment, the experiment cycle is long, and the interpretation of the results is not intuitive enough.

Therefore, the above two methods are not suitable for popularization and application in aquaculture production.

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