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Application of bacteroides fragilis in preparation of composition for treating constipation

A Bacteroides fragilis, a technology for treating constipation, used in applications, food preparation, drug combinations, etc.

Inactive Publication Date: 2013-05-29
广东知光生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of the bacterial strain adopted in the present invention has not yet been reported, so its application has very broad prospects for development

Method used

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  • Application of bacteroides fragilis in preparation of composition for treating constipation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Isolation of Bacteroides fragilis from infant feces

[0028] Weigh 0.5 g of stool according to the "normal flora test method", put it in a test tube filled with 4.5 ml of normal saline, and serially dilute it to 10 -1 ~10 -6 , from high concentration to low concentration on the Bd medium plate, placed in an anaerobic gas box and anaerobic gas bag for anaerobic culture. Store at 37°C, open the can for inspection after 2-7 days. Select a single colony for pure culture, staining microscopy, culture characteristics, and physiological and biochemical testing.

[0029] (2) Enrichment culture of Bacteroides fragilis and preparation of bacterial solution

[0030] Select the purified single colony and inoculate it into the improved tryptone broth for enrichment. The obtained bacterial solution is centrifuged and precipitated at a speed of 3000r / min for 15 minutes. The supernatant and precipitate are removed and washed with normal saline. McFarland turbidimetric tub...

Embodiment 2

[0032] Bacteroides fragilis virulence test

[0033] (1) Acute toxicity test in mice

[0034] Sixty C57BR / C mice (half male and half female) weighing 22±2 g were divided into high-dose group, low-dose group and control group, 20 in each group. In the high-dose group, 10 12 cfi / ml bacterial solution 0.5ml, the low dose group was fed 10 times a day 9 cfi / ml bacterial solution 0.5ml, the control group was fed with 0.5ml normal saline every day, observed for 14 days.

[0035] Experimental results: The mice grew normally, no death was observed, and the LD50 was undetectable.

[0036] (2) Rat toxicity test

[0037] Take 30 SD rats (half male and half female) of 150±20 g, and divide them into 3 groups: high and low dose group and control group. In the high-dose group, 10 12 cfi / ml bacterial solution 2ml, the low dose group was fed 10 times a day 9 cfi / ml bacterial solution 2ml, the control group was fed with 2ml normal saline every day. Continuous observation for 14 days, ...

Embodiment 3

[0042] The therapeutic effect of Bacteroides fragilis on constipation

[0043] Before the modeling, the animals were fasted and given water, and then given 25mg / kg of compound diphenoxylate (prepared with physiological sodium chloride solution) for intragastric administration, each 0.5ml Bid, and observed the time of the first defecation and the defecation within 12 hours. count and defecation weight.

[0044] Constipated mice were treated with 10 8 cfi / ml, 10 9 cfi / ml, 10 10 The cfi / ml bacterial solution was administered into the stomach, 0.5ml Bid each time, and the blank control group was given an equal volume of normal saline, Bid. The time of the first defecation, the number of defecated pellets and defecation weight within 12 h were observed (10 mice in each group). The result is as follows:

[0045]

[0046] *P<0.05 compared with blank control group.

[0047] The results of the experiment showed that compared with the mice in the normal saline gavage group, ...

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Abstract

The invention relates to an application of bacteroides fragilis in the preparation of a composition for treating constipation. The bacteroides fragilis is used for preparing a carrier, and also can be used for preparing the composition of a food or a drug containing the microorganism. An experiment proves that the bacteroides fragilis has a good treatment effect on constipation; the bacteroides fragilis not only can supply physiological bacteria for an intestinal tract, corrects flora disturbance of the intestinal tract during constipation, and promotes digestive absorption of the food; various organic acids are also formed in the process of metabolization of the intestinal tract; and an acidic environment is built in the intestinal tract. Therefore, the pH value of the intestinal tract can be reduced; normal peristalsis of the intestinal tract is facilitated; meanwhile, spoilage organisms are inhibited; the adverse effect of harmful metabolites on the intestinal tract generated by the spoilage organisms of the intestinal track is reduced; enteroplegia is prevented; and the prepared drug is good in effect of treating the constipation, free of side effect, safe and reliable.

Description

technical field [0001] The invention relates to the use of Bacteroides fragilis, in particular to using Bacteroides fragilis to prepare a carrier and adding it to food or medicine to treat constipation, and belongs to the technical field of microbial application of Bacteroides fragilis. Background technique [0002] Constipation is the most common digestive system disease clinically, and its incidence is different in different regions and reports by scholars. Generally speaking, the west is higher than the east, women are higher than men, the elderly are higher than young people, and children are sick Also ubiquitous. According to the survey, the incidence of constipation in Western populations is about 6% to 23%. my country has a vast territory, and the survey results of the incidence vary from place to place: 6.07% of urban and rural residents aged 18-70 in Beijing suffer from constipation. The male-to-female ratio is 1:4.59; the prevalence rate of constipation among the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61P1/10A23C9/152A23C9/13A23C19/09A23G9/36A23L1/30A61K35/741
Inventor 智发朝白杨刘洋洋
Owner 广东知光生物科技有限公司
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