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Baby bifidobacterium and preparation thereof

A technology for Bifidobacterium infantis and preparations, which is applied to bacteria, medical raw materials derived from bacteria, medical preparations with inactive ingredients, etc. requirements, etc., to achieve the effect of improving the viable bacteria rate and its storage time, quality and production conditions, and enhancing the activity of seeds.

Active Publication Date: 2013-06-05
SHANGHAI SINE PHARMA LAB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The formula of the medium for cultivating bifidobacteria currently used is relatively complicated, the quality and production conditions are not easy to control, and there are many types of raw materials, which are not suitable for large-scale fermentation, and the fermentation volume is low, so it is difficult to meet the production requirements; there are many kinds of freeze-drying protective agents. , the proportions are different, the freeze-drying process is immature, the live bacteria rate of freeze-dried varieties is low, and the storage time is not long, which directly affects the quality of bifidobacteria preparations

Method used

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  • Baby bifidobacterium and preparation thereof
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  • Baby bifidobacterium and preparation thereof

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preparation example Construction

[0067] The present invention also provides a kind of preparation method of freeze-dried powder preparation of the present invention, described method comprises the following steps:

[0068] (a) Inoculate and cultivate the Bifidobacterium infantis bacterial classification of the present invention in a primary seed liquid culture medium to obtain a primary inoculation product;

[0069] (b) inoculating and culturing the primary inoculation product into the secondary seed liquid medium to obtain the secondary inoculation product;

[0070] (c) the secondary inoculation product is connected to the culture medium in the 50L fermenter to obtain the fermentation product;

[0071] (d) connecting the fermented product obtained in step (c) to the culture medium of the fermenter to obtain the fermented product;

[0072] (e) centrifuging the fermentation product obtained in step (d), mixing it with the protective agent, and freeze-drying to obtain a freeze-dried powder preparation.

[007...

Embodiment 1

[0082] Embodiment 1. prepare the bacterial classification of bifidobacterium infantis

[0083] A. Prepare PYG liquid medium (proportion 1). Use 50mL pure water to dissolve tryptone (MERCK KGAA, Germany), yeast extract (OXID, UK), L-cysteine ​​hydrochloride (Shanghai Kangjie Biotechnology Co., Ltd.), glucose (Shanxi Wang Biochemical Technology Co., Ltd.), calcium chloride (Sinopharm Chemical Reagent Co., Ltd.), magnesium sulfate (Sinopharm Chemical Reagent Co., Ltd.), dipotassium phosphate (Sinopharm Chemical Reagent Co., Ltd.), potassium dihydrogen phosphate (Sinopharm Chemical Reagent Co., Ltd.) to obtain a suspension, and adjust the pH of the medium with a little sodium hydroxide solution. Put the culture medium in the Erlenmeyer flask, seal the mouth of the flask and place it in a sterilizer for sterilization at 121°C for 15 minutes. Add fresh feces of healthy babies (taken from China Welfare Institute International Peace Maternal and Child Health Hospital) to the PYG liq...

Embodiment 2

[0091] Embodiment 2. prepare the primary seed liquid culture medium of Bifidobacterium infantis

[0092] Use 300 mL of pure water to dissolve tryptone (MERCK KGAA, Germany), beef extract (OXID, UK), yeast extract (OXID, UK), lactose (DMV-Fonterra Excipients, Germany), glucose (Shandong Xiwang Biochemical Technology Co., Ltd. Co., Ltd.), dipotassium hydrogen phosphate (Sinopharm Group Chemical Reagent Co., Ltd.), magnesium sulfate (Sinopharm Group Chemical Reagent Co., Ltd.), ferrous sulfate (Sinopharm Group Chemical Reagent Co., Ltd.), L-cysteine ​​hydrochloride ( Shanghai Kangjie Biotechnology Co., Ltd.) to obtain a suspension, and adjust the pH of the medium with a little sodium hydroxide solution. Put the culture medium in the Erlenmeyer flask, seal the mouth of the flask and place it in a sterilizer for sterilization at 121°C for 15 minutes.

[0093] Table 6. Ratio of primary seed liquid culture medium

[0094]

[0095]

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Abstract

The invention relates to baby bifidobacterium, a medicine combination, application of the baby bifidobacterium and the medicine combination and manufacturing method of the baby bifidobacterium and the medicine combination and provides a baby bifidobacterium bacterial strain IFA09 and a preservation number of the baby bifidobacterium bacterial strain is CCTCC NO:M2011310. The baby bifidobacterium has high activity and is capable of effectively treating gastrointestinal neurosis diseases. In addition, a vaccination liquid culture medium enables activity of a vaccination to be enhanced, a fermentation medium is stable in composition, quality and production conditions are easy to control, industrialized production at 500L can be achieved, high intensity culture of 1010cfu / mL can be achieved and production requirements can be completely satisfied. The invention provides a freezing and drying protecting agent and freezing and drying technology which can improve a viable bacteria rate and prolong storage time of the freezing and drying protecting agent and thus stability of the agent is improved.

Description

technical field [0001] The invention relates to a new strain of Bifidobacterium infantis, its preparation method and its use in treating gastrointestinal dysfunction. Background technique [0002] Bifidobacterium is an important physiological bacterium in human and animal intestines. It plays an important role in promoting the development of the human body, maintaining and improving immunity, and delaying the aging of the body. Nearly a hundred years of research has proved that bifidobacteria are the dominant flora in the human intestinal tract. During the growth process of the human body, due to diseases, aging and other reasons, the number of bifidobacteria in the body and the occupancy rate of the total bacteria gradually decrease. Therefore, some people regard the number of bifidobacteria in the body as one of the signs of health. Bifidobacteria account for 92% of the total number of bacteria in the intestinal tract of newborns, which decrease with age and disappear co...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02A61K35/74A61K9/19A61K47/46A61P1/00A61P1/12A61P35/00A61P37/02C12R1/01A61K35/745
Inventor 陈蓓张军东王莎莎汪忠慧于鸿晶沈颐涵
Owner SHANGHAI SINE PHARMA LAB
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