Preparation method of fish gelatin high-molecular material

A polymer material and gelatin technology, which is applied in the field of preparation of fish gelatin polymer materials, can solve the problems of not paying attention to preventing the denaturation and degradation of fish collagen, poor performance of fish gelatin products, cumbersome processing process, etc. The effect of denaturation, short extraction time and simple extraction process

Active Publication Date: 2013-06-12
JIMEI UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, although Southwest University has reported a method of producing high-quality fish skin gelatin through ultra-high pressure technology-assisted treatment combined with thermal extraction (see Chinese patent 200910251061.4), and Chengdu Haikang Biotechnology Co., Ltd. has provided an industrialized production of fish skin gelatin technology (see Chinese patent 201210186309.5), but there are still some problems in the application of these technologies, such as cumbersome pretreatment process and long production cycle; the extraction process does not pay attention to preventing the denaturation and degradation of fish collagen under the action of endogenous enzymes and heat, resulting in Prepared fish gelatin products with poor performance

Method used

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  • Preparation method of fish gelatin high-molecular material
  • Preparation method of fish gelatin high-molecular material

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Add 5 L of 0.1% NaOH solution containing 0.2% sodium dodecyl sulfate (SDS) and 0.5% sodium percarbonate to 1 kg of tilapia fish scales, soak for 24 hours at 4°C, rinse with tap water, and drain. Add 1L of pure water, use citric acid or sodium hydroxide to adjust the pH of the fish scale aqueous solution to a certain value, then rapidly raise the temperature to 60°C, heat and keep warm for extraction for 1 hour, filter with filter paper, freeze-dry after cooling and solidifying, and obtain tilapia fish scales gelatin. The gelatin components of tilapia fish scales were determined by SDS-PAGE ( figure 1 ), the gel formation of gelatin can be measured by BLOOM (Table 1).

[0026] Table 1 Effect of extraction pH on the gel freezing force of tilapia scale gelatin

[0027]

Embodiment 2

[0029] Add 5 L of 0.1% NaOH solution containing 0.2% SDS and 0.5% sodium percarbonate to 1 kg of tilapia fish scales, soak at 4°C for 24 hours, rinse with tap water and drain, then add 1 L of pure water to achieve a total weight of About 2kg, add 60mg of soybean extract (the specific activity of the inhibitor is 10000U / mg), adjust the pH of the solution to 9 with sodium hydroxide, then rapidly raise the temperature to 60°C, heat and keep it for extraction for a certain period of time, filter with filter paper, cool and solidify Then freeze-dry to obtain tilapia scale gelatin. At the same time, gelatin prepared without adding soybean extract was used as a control group. The results of SDS-PAGE determination of gelatin components are as follows figure 2 shown.

Embodiment 3

[0031] Add 10L of 0.4% NaOH solution containing 0.5% alkylphenol polyoxyethylene ether and 1.0% sodium hypochlorite to 1kg of cod skin, soak at 4°C for 12 hours, rinse with tap water, drain, and add 0.5L of pure When the total weight of water reaches about 2kg, add 100mg of soybean extract (the specific activity of the inhibitor is 10000U / mg) and 4g of trehalose, adjust the pH of the fish skin aqueous solution to 5 with adipic acid, and heat up to 70°C quickly after crushing. Heat and keep it warm for 1 hour, filter the obtained gelatin solution with a filter cloth, and after cooling and solidifying, cut into thin strips and blow dry at 15° C. to obtain cod skin gelatin. Table 2 shows the test results of the obtained gelatin gel properties.

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Abstract

The invention discloses a preparation method of a fish gelatin high-molecular material, relates to a fish gelatin high-molecular material, and provides a preparation method which is good in gelation performance for a fish gelatin high-molecular material. The preparation method comprises the following steps of: 1) raw material pretreatment: by taking fish skins and fish scales as raw materials, soaking the raw materials with sodium hydroxide solution containing a surfactant and an oxidant, rinsing cleanly, adding pure water, adjusting the pH value of the solution to 5-6 by polybasic carboxylic acid, and then stirring and crushing the raw materials into extraction raw materials for gelatins; 2) gelatin extraction: adding a protease inhibitor and a protein thermal denaturation protectant in the raw materials pretreated in the step 1), heating and extracting gelatin solution; and 3) drying: filtering the gelatin solution obtained in the step 2) to remove impurities such as melanin, cooling and condensing, drying at a low temperature and crushing, and then preparing the fish gelatin high-molecular material powder. The preparation method is simple in process, short in extraction time, and beneficial to popularization for fish gelatin industrialized production.

Description

technical field [0001] The present invention relates to a gelatin polymer material, in particular to a kind of fish that uses fish skin and fish scales as raw materials, adds protease inhibitors, protein denaturation protective agents, and controls extraction temperature and pH to prevent the degradation and thermal denaturation of fish collagen. A method for preparing a gelatin-like polymer material. Background technique [0002] Collagen is a fibrous protein and one of the most critical raw materials in the biotechnology industry. It can be widely used in medical materials, cosmetics, food industry and other fields. Gelatin is a natural polymer compound derived from thermal denaturation of collagen. It has good gel-forming properties and is often used as an additive in food production to increase the elasticity, viscosity and stability of the product. At present, more than 90% of the gelatin in the world comes from the skin and bones of pigs and cattle. However, due to th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09H3/00C09H3/02C09H9/00
Inventor 翁武银
Owner JIMEI UNIV
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